Strengths and Silences: The Experiences of Lesbian, Gay, Bisexual and Transgender Students in Rural and Small Town Schools

For more than 20 years, GLSEN has worked to make schools safer for all students; it has sought specifically to reduce the bullying and harassment targeted at students' sexual orientation, gender identity, and gender expression. For lesbian, gay, bisexual, and transgender (LGBT) students across the country, violence and harassment experienced in school affect their ability to learn. Although schools in urban areas are typically regarded as more violent or dangerous than schools in other areas, findings from our National School Climate Surveys consistently show that it is most often rural schools that may pose the greatest threats for LGBT students. It may be that community characteristics, such as religious and cultural traditions, income, and educational levels, influence individual beliefs and attitudes toward LGBT people in these areas. It may also be that a lack of positive LGBT-related school resources negatively affects LGBT students' school engagement and academic performance, particularly if they also experience bullying and harassment. Although research on the educational experiences of LGBT youth has grown considerably over the past 25 years, less is known about rural students specifically. This research report examines the experiences of LGBT students in small town and rural areas on matters related to biased language in schools, school safety, harassment and victimization, educational outcomes, school engagement, and LGBT-related resources and support. It also examines the prevalence and utility of LGBT-related resources in rural schools. Finally, this report concludes by advocating for more intentional policies, measures, and programs that protect LGBT students

The 2011 National School Climate Survey: The Experiences of Lesbian, Gay, Bisexual and Transgender Youth in Our Nation's Schools

In 1999, GLSEN identified the need for national data on the experiences of lesbian, gay, bisexual, and transgender (LGBT) students and launched the first National School Climate Survey (NSCS). At the time, the school experiences of LGBT youth were under-documented and nearly absent from national studies of adolescents. For more than a decade, the biennial NSCS has documented the unique challenges LGBT students face and identified interventions that can improve school climate. The survey explores the prevalence of anti-LGBT language and victimization, the effect that these experiences have on LGBT students' achievement and well-being, and the utility of interventions in lessening the negative effects of a hostile school climate and promoting a positive educational experience. The survey also examines demographic and community-level differences in LGBT students' experiences.The NSCS remains one of the few studies to examine the school experiences of LGBT students nationally, and its results have been vital to GLSEN's understanding of the issues that LGBT students face, thereby informing our ongoing work to ensure safe and affirming schools for all.In our 2011 survey, we examine the experiences of LGBT students with regard to indicators of negative school climate:hearing biased remarks, including homophobic remarks, in school;feeling unsafe in school because of personal characteristics, such as sexual orientation, gender expression, or race/ethnicity;missing classes or days of school because of safety reasons; andexperiencing harassment and assault in school.We also examine:the possible negative effects of a hostile school climate on LGBT students' academic achievement, educational aspirations, and psychological well-being;whether or not students report experiences of victimization to school officials or to family members and how these adults address the problem; andhow the school experiences of LGBT students differ by personal and community characteristics.In addition, we demonstrate the degree to which LGBT students have access to supportive resources in school, and we explore the possible benefits of these resources, including:Gay-Straight Alliances (GSAs) or similar clubs;anti-bullying/harassment school policies and laws;supportive school staff; andcurricula that are inclusive of LGBT-related topics.Given that GLSEN has more than a decade of data, we examine changes over the time on indicators of negative school climate and levels of access to LGBT-related resources in schools

THE NATURAL HISTORY AND CAPTIVE HUSBANDRY OF THE SALT CREEK TIGER BEETLE, \u3ci\u3eCicindela (=Ellipsoptera) nevadica lincolniana\u3c/i\u3e (COLEOPTERA: CARABIDAE).

Tiger beetles have long been admired for their mix of beauty, speed, and ferocious hunting abilities (Pearson 2011). The tiger beetles are some of the fastest insects on the planet with the Australian species, Cicindela hudsoni being clocked at 2.5meters per second (Merrit 1999). The tiger beetles run so fast that they are temporarily blinded when they are engaged in the high speed pursuit of their prey (Friedlander 2014). However, despite their speed, and the joy they bring to many enthusiasts, tiger beetles are unable to outrun the destruction and degradation of their habitats by human activities, and an estimated 15 percent of the 255 described species and subspecies of North American tiger beetles are now threatened with extinction (Pearson 2011). Several species of tiger beetle are very niche specialized and will only inhabit certain areas where the conditions are just right for their survival (Pearson 2006). The saline wetlands of Lancaster and Saunders counties located in eastern Nebraska are home to one such beetle. An endemic subspecies of the Nevada Tiger Beetle, Cicindela (=Ellipsoptera) nevadica, calls these saline wetlands home. This beetle is aptly named the Salt Creek Tiger beetle, Cicindela (=Ellipsoptera) nevadica lincolniana, due to its presence only along Little Salt Creek and associated tributaries (Spomer, et al. 2007). The olive-colored, 5mm long beetles are dependent on the saline wetlands, and due to the rapid destruction of their habitat by farmers and developers, are now threatened with extinction (Spomer, et al.2007). The population numbers for the C. n. lincolniana range from 150-1000 adults a year, making this species one of the most endangered insects in North America (Higley & Spomer 2001). The following paper gives a brief overview of the natural history of the C. n. lincolniana and outlines the efforts of the Omaha’s Henry Doorly Zoo and Aquarium in its attempt to captive rear this species, and save Nebraska’s native tigers and the wetlands they call home

State Dependence of Stimulus-Induced Variability Tuning in Macaque MT

Behavioral states marked by varying levels of arousal and attention modulate some properties of cortical responses (e.g. average firing rates or pairwise correlations), yet it is not fully understood what drives these response changes and how they might affect downstream stimulus decoding. Here we show that changes in state modulate the tuning of response variance-to-mean ratios (Fano factors) in a fashion that is neither predicted by a Poisson spiking model nor changes in the mean firing rate, with a substantial effect on stimulus discriminability. We recorded motion-sensitive neurons in middle temporal cortex (MT) in two states: alert fixation and light, opioid anesthesia. Anesthesia tended to lower average spike counts, without decreasing trial-to-trial variability compared to the alert state. Under anesthesia, within-trial fluctuations in excitability were correlated over longer time scales compared to the alert state, creating supra-Poisson Fano factors. In contrast, alert-state MT neurons have higher mean firing rates and largely sub-Poisson variability that is stimulus-dependent and cannot be explained by firing rate differences alone. The absence of such stimulus-induced variability tuning in the anesthetized state suggests different sources of variability between states. A simple model explains state-dependent shifts in the distribution of observed Fano factors via a suppression in the variance of gain fluctuations in the alert state. A population model with stimulus-induced variability tuning and behaviorally constrained information-limiting correlations explores the potential enhancement in stimulus discriminability by the cortical population in the alert state.Comment: 36 pages, 18 figure

Out Online: The Experiences of Lesbian, Gay, Bisexual and Transgender Youth on the Internet

This report examines the online experiences of LGBT students in 6-12th grade. LGBT youth experience nearly three times as much bullying and harassment online as non-LGBT youth, but also find greater peer support, access to health information and opportunities to be civically engaged

Rapid analytical methods for the microalgal and cyanobacterial biorefinery: application on strains of industrial importance.

To realise the potential of microalgae in the biorefinery context, exploitation of multiple products is necessary for profitability and for bioproduct valorisation. Appropriate analytical tools are required for growth optimisation, culture monitoring and quality control purposes, with safe, low-tech and low-cost solutions favourable. Rapid, high-throughput and user-friendly methodologies were devised for a) determination phycobiliproteins, chlorophylls, carotenoids, proteins, carbohydrates and lipids and b) qualitative and quantitative carotenoid profiling using UPLC-PDA-MSE. The complimentary methods were applied on 11 commercially important microalgal strains including Prasinophytes, Haptophytes and cyanobacteria, highlighting the suitability of some strains for coproduct exploitation and the methods utility for research and industrial biotechnology applications. The UPLC method allowed separation of 41 different carotenoid compounds in < 15 minutes. Simple techniques are described for further quantification and comparison of pigment profiles, allowing for easy strain selection and optimisation for pigment production, with suitability for biotechnological or biomedical applications

HIV Status Does Not Affect the Outcome of Autologous Stem Cell Transplantation (ASCT) for Non-Hodgkin Lymphoma (NHL)

Randomized trials comparing autologous stem cell transplant (ASCT) to conventional chemotherapy have demonstrated superior survival among HIV-negative ASCT patients with relapsed non-Hodgkin lymphoma (NHL). Recent trials explored the feasibility of ASCT in the HIV setting. Although these studies have shown that ASCT in HIV-positive NHL patients (HIVpos-NHL) is well tolerated, the impact of HIV infection on long-term transplant outcome is not well characterized. Ongoing comparison of long-term survival following ASCT in HIVpos-NHL patients and HIVneg-NHL patients will allow investigators to explore whether there should be inclusion of HIVpos-NHL patients in ASCT trials. To study long-term outcome we conducted a single-institution matched case-controlled study in HIVpos-NHL patients (cases) and HIVneg-NHL patients (controls). Twenty-nine patients with HIVpos-NHL were matched with HIVneg-NHL controls on sex, time to ASCT, year of transplant, histology, age, disease status, number prior regimens, and conditioning regimen. Nonrelapse mortality (NRM) was similar: 11% (95% confidence interval [CI]: 4%-28%) in HIVpos-NHL patients and 4% (95% CI: 1%-25%) in HIVneg-NHL controls (P = .18). Two-year disease-free survival (DFS) for the HIVpos-NHL patients was 76% (95% CI: 62%-85%) and 56% (95% CI: 45%-66%) for the HIVneg-NHL controls (P = .33). Overall survival was also similar; the 2-year point estimates were 75% (95% CI: 61%-85%) and 75% (95% CI: 60%-85%), respectively (P = .93), despite inclusion of more poor risk HIVpos-NHL patients. These results provide further evidence that HIV status does not affect the long-term outcome of ASCT for NHL, and therefore HIV status alone should no longer exclude these patients from transplant clinical trials

Indolinyl-Thiazole Based Inhibitors of Scavenger Receptor-BI (SR-BI)-Mediated Lipid Transport

A potent class of indolinyl-thiazole based inhibitors of cellular lipid uptake mediated by scavenger receptor, class B, type I (SR-BI) was identified via a high-throughput screen of the National Institutes of Health Molecular Libraries Small Molecule Repository (NIH MLSMR) in an assay measuring the uptake of the fluorescent lipid DiI from HDL particles. This class of compounds is represented by ML278 (17–11), a potent (average IC50 = 6 nM) and reversible inhibitor of lipid uptake via SR-BI. ML278 is a plasma-stable, noncytotoxic probe that exhibits moderate metabolic stability, thus displaying improved properties for in vitro and in vivo studies. Strikingly, ML278 and previously described inhibitors of lipid transport share the property of increasing the binding of HDL to SR-BI, rather than blocking it, suggesting there may be similarities in their mechanisms of action

Gene Expression Profiling of Bronchoalveolar Lavage Cells Preceding a Clinical Diagnosis of Chronic Lung Allograft Dysfunction.

BackgroundChronic Lung Allograft Dysfunction (CLAD) is the main limitation to long-term survival after lung transplantation. Although CLAD is usually not responsive to treatment, earlier identification may improve treatment prospects.MethodsIn a nested case control study, 1-year post transplant surveillance bronchoalveolar lavage (BAL) fluid samples were obtained from incipient CLAD (n = 9) and CLAD free (n = 8) lung transplant recipients. Incipient CLAD cases were diagnosed with CLAD within 2 years, while controls were free from CLAD for at least 4 years following bronchoscopy. Transcription profiles in the BAL cell pellets were assayed with the HG-U133 Plus 2.0 microarray (Affymetrix). Differential gene expression analysis, based on an absolute fold change (incipient CLAD vs no CLAD) &gt;2.0 and an unadjusted p-value ≤0.05, generated a candidate list containing 55 differentially expressed probe sets (51 up-regulated, 4 down-regulated).ResultsThe cell pellets in incipient CLAD cases were skewed toward immune response pathways, dominated by genes related to recruitment, retention, activation and proliferation of cytotoxic lymphocytes (CD8+ T-cells and natural killer cells). Both hierarchical clustering and a supervised machine learning tool were able to correctly categorize most samples (82.3% and 94.1% respectively) into incipient CLAD and CLAD-free categories.ConclusionsThese findings suggest that a pathobiology, similar to AR, precedes a clinical diagnosis of CLAD. A larger prospective investigation of the BAL cell pellet transcriptome as a biomarker for CLAD risk stratification is warranted