Microfluidic systems for the analysis of the viscoelastic fluid flow phenomena in porous media

In this study, two microfluidic devices are proposed as simplified 1-D microfluidic analogues of a porous medium. The objectives are twofold: firstly to assess the usefulness of the microchannels to mimic the porous medium in a controlled and simplified manner, and secondly to obtain a better insight about the flow characteristics of viscoelastic fluids flowing through a packed bed. For these purposes, flow visualizations and pressure drop measurements are conducted with Newtonian and viscoelastic fluids. The 1-D microfluidic analogues of porous medium consisted of microchannels with a sequence of contractions/ expansions disposed in symmetric and asymmetric arrangements. The real porous medium is in reality, a complex combination of the two arrangements of particles simulated with the microchannels, which can be considered as limiting ideal configurations. The results show that both configurations are able to mimic well the pressure drop variation with flow rate for Newtonian fluids. However, due to the intrinsic differences in the deformation rate profiles associated with each microgeometry, the symmetric configuration is more suitable for studying the flow of viscoelastic fluids at low De values, while the asymmetric configuration provides better results at high De values. In this way, both microgeometries seem to be complementary and could be interesting tools to obtain a better insight about the flow of viscoelastic fluids through a porous medium. Such model systems could be very interesting to use in polymer-flood processes for enhanced oil recovery, for instance, as a tool for selecting the most suitable viscoelastic fluid to be used in a specific formation. The selection of the fluid properties of a detergent for cleaning oil contaminated soil, sand, and in general, any porous material, is another possible application

Comparison of Short-Term Estrogenicity Tests for Identification of Hormone-Disrupting Chemicals

The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods

Evaluation of in vitro antiviral activity of a brown alga (Cystoseira myrica) from the Persian Gulf against herpes simplex virus type 1

The hot water extract of a brown marine alga, Cystoseira myrica, from the Persian Gulf was evaluated as an antiviral compound against KOS strain of HSV-1 in cell culture. The extract exhibited antiviralactivity against herpes simplex virus type 1 (HSV-1) not only during absorption of virus to the cells, but also on post attachment stages of virus replication. The water extract of C. myrica was sterilized byfiltration and autoclaving, respectively. The IC50 for filtered extract was 99 ìg/ml and the IC50 for autoclaved extract was 125 ìg/ml. Based on resulted selectivity index (SI) values of the extracts, whichwere 33.4 and 28.2 for filtered and autoclaved extracts, respectively, we found that the antiviral compound(s) in the water extract of C. myrica to be heat stable. Also, the SI values for inhibition of thepost attachment stages of HSV-1 replication were 23.1 and 21.7 for filtered and autoclaved extracts, respectively. The IC50 in this phase of study were 143 and 162 ìg/ml for filtered and autoclaved extracts,respectively. Therefore, C. myrica could be a good candidate as a natural source for anti-HSV-1 compound(s) isolation

Heat in the Streets

Heat stress from rising temperatures in the workplace is an urgent public health issue. The absence of canopy cover, excessive built-up areas with heat-reflective materials such as glass facades and concrete paving, the absence of shade provided by surrounding buildings, the width of streets, and traffic occupancy often aggravate heat stress in cities. This paper presents the outcomes of a research study to map, analyse, and visualise the lived experience of climate-exposed outdoor workers. The project sought to understand how experience data of heat-exposed urban workers can be communicated using digital tools and environmental sensors to derive evidence-based suggestions for developing heat-sensitive urban environments. Focusing on bicycle delivery couriers and outdoor council workers, the project draws on quantitative (temperature, humidity, and geo-location) and qualitative data (time worked and psychophysiological responses to heat) from outdoor urban workers. Minnow sensors, Strava (geo- location mapping), analogue intake and exit interviews, Google Street View, and online surveys were deployed for data acquisition, correlation, and prototyping of a real-time updating digital dashboard that served as a visual narrative of summer heat stress experienced by Sydney’s essential outdoor workers. The dashboard is instrumental in revealing heat stress hotspots and corresponding opportunities for urban interventions (e.g., heat refuges, shade, landscaping) to mitigate urban heat effects while simultaneously revealing the lived experience data of the participant outdoor workers. A citizen science initiative, the research is instrumental in communicating the impact of the spatial, social, and policy landscape on critical climate emergencies to a broader audience

Study on potential of some solid natural substances in production of Beauveria bassiana (Ascomycota, Cordycipitaceae) conidia

In this study, two fungus isolates, Beauveria bassiana EUT105 and B. bassiana EUT116, were evaluated for production of aerial conidia on wheat, wheat flour, wheat bran, rice flour, rice bran, rice paddy, millet and corn flour using two phasic liquid-solid system. Data analysis showed that there are significant differences among treatments (simple media) in both isolates. In B. bassiana EUT105, the maximum and minimum productions were achieved on wheat bran, 2.3 Ã 1010 conidia/g and on rice paddy, 1.5 Ã 109 conidia/g of medium, respectively. In B. bassiana EUT116, wheat bran with 6.1 Ã 109 conidia/g and millet with 8 Ã 108 conidia/g had the maximum and minimum productions, respectively. Effect of adding nutritional complementary compounds to the growth media was significant in increasing of conidial yield. Related results showed that adding permeate increased the production but molasses did not it in comparison with control in both isolates. Wheat bran plus permeate had the maximum production, 5 Ã 1010 and 4.6 Ã 1010 conidia/g in B. bassiana EUT105 and EUT116, respectively and minimum production was obtained with rice flour plus molasses, 1.5 Ã 108 and 6 Ã 108 conidia/g in B. bassiana EUT105 and EUT116, respectively

Report of a thelytokous population of Lysiphlebus fabarum (Hym.: Aphidiidae) from Iran

به منظور جمع‌آوری و شناسایی پارازیتوئیدهای شته‌ی سیاه باقلا، Scopoli Aphis fabae، از مزارع باقلای منطقه‌ی چورزق زنجان در اواسط خرداد‌ماه 1386 نمونه‌برداری به عمل آمد. در بین نمونه‌ها، جمعیت ماده‌‌زای زنبور پارازیتوئید Lysiphlebus fabarum (Marshall) از خانواده‌ی Aphidiidae شناسایی شد که برای اولین‌بار از ایران گزارش می‌‌شود. در زیر برخی ازویژگی‌‌های مهم تولید مثلی و چگونگی پراکنش جغرافیایی این جمعیت ارایه شده است