4 research outputs found
Biophysical characterization, nanoscale composition and cell uptake studies of pH-sensitive drug delivery systems
Nanocarrier-based chemotherapy is one of the few nanotechnology-based medical therapies that reached the clinics. This happened already in 1995, when the commercial anti cancer drug delivery system DOXILÂź was introduced in the market [1]. Albeit these early developments, still today nanotechnology-based drug delivery systems are far from reaching optimal selectivity and controlled release ability.
In our study we use different liposomal formulations designed for pH-sensitive drug release and study their biophysical characteristics, when used for trafficking paclitaxel (PTX) and doxorubicin (DOX), both widely used chemotherapeutic anti-cancer drugs.
Our work describes a combined spectroscopy and imaging approach to evaluate the biophysical properties of liposomal formulations. We study the nanoscale composition of the nanocarriers using molecular rulers in a fluorescence quenching assay, and analyze the cell uptake characteristics based on the autofluorescence of DOX using confocal microscopy.info:eu-repo/semantics/publishedVersio
Smart drug delivery systems for cancer therapy
Ideal nanocarrier-based therapy, contains first a controlled mechanism for drug delivery, to minimize side effects in healthy tissues, and second has the ability to provide a controlled drug release to extend the therapeutic duration of the therapeutic treatment. Nanocarrier based chemotherapy is one of the few nanotechnology-based medical therapies that reached the clinics, already in 1995, when the commercial anti cancer drug delivery system DOXILÂź was introduced in the market, but available systems are far from optimal selectivity and controlled release. Our work describes a combined spectroscopy and imaging study to evaluate the two aspects of a smart drug delivery system. In our study we used these DODAX:MO (1:2) formulations with a diameter of approx. 100 nm to study the biophysical characteristics when used for trafficking paclitaxel (PTX) and doxorubicin (DOX), both widely used chemotherapeutic anti-cancer drugs. Taking advantage of the of DOX intrinsic fluorescence also the cell uptake is studied for DODAB:MO (1:2) liposomal formulations.info:eu-repo/semantics/publishedVersio