The influence of mechanochemical modification on prevention of toxic ability of humic acids towards phenanthrene in aquatic environment

Целью работы является количественная оценка взаимодействия фенантрена с модифицированными гуминовыми кислотами в водной среде. Изменение структуры и свойств ГК после модификации было изучено методами ИК-, {1}Н ЯМР - спектроскопии и потенциометрического титрования. Доказано что применение тиомочевины в качестве модифицирующего агента значительно увеличивает связывающую способность гуминовых кислот по отношению к фенантрену

Research of efficiency of means of the physical recreation of preschool children

Results of pedagogical experiment on research of efficiency sports рекреативных actions in a day regimen of preschool educational institution are presented in article. Dynamics of indicators of a physical and psycho-emotional condition of the senior preschool children under influence a fitball gymnastics, finger-type and аrthritic gymnastics, a hop-bol, sports dosug is discussedВ статье представлены результаты педагогического эксперимента по исследованию эффективности физкультурно-рекреативных мероприятий в режиме дня дошкольного образовательного учреждения. Обсуждается динамика показателей физического и психо-эмоционального состояния старших дошкольников под воздействием фитбол-гимнастики, пальчиковой и суставной гимнастики, хоп-бола, спортивных досуго

The influence of mechanochemical modification on prevention of toxic ability of humic acids towards phenanthrene in aquatic environment

The aim of the research work is to quantify interaction between phenanthrene with modified humic acids in aquatic environment. The changes in the structure and properties of humic acids after modifications were studied with 1Н NMR spectroscopy and potentiometric titration methods. Our research demonstrates that the application of thiourea as a modified agent increases the binding capacity of humic acids towards phenanthrene

THE INFLUENCE OF MILK-CLOTTING ENZYMES ON THE FUNCTIONAL PROPERTIES OF PIZZA-CHEESES

The effect of the type and dose of milk-clotting enzymes (Chy-max® M based on recombinant camel chymosin, Fromase® TL based on Rhizomucor miehei protease) on the physicochemical, functional properties and shelf life of pizza-cheeses was studied. When using a low dose of milk-clotting enzymes (MCE) for milk coagulation (250–1100 IMCU per 100 kg of milk), cheeses were obtained with an increased moisture content (55–57%), excessive acidity (pH 4.8–4.9) and texture defects (incoherent, crumbly, with separation of free moisture). This is due to the formation of a weak curd, which releases moisture poorly during processing. The use of an increased dose of MCE makes it possible to obtain a denser curd, better releasing moisture. Cheese produced with a high dose of milk-clotting enzymes (2000–2800 IMCU per 100 kg of milk) had a lower moisture content (52–53%) and lower acidity (pH 5.0–5.1). The protein matrix is more hydrated in these cheeses, which ensures its better water holding capacity and a more homogeneous and cohesive texture. The use of an increased dose of MCE with a high total proteolytic activity (Fromase) gives undesirable consequences in the form of accelerated proteolysis of cheese mass proteins, rapid loss of functional properties of the cheese, and a decrease in the shelf life of cheese (less than 60 days). Cheese production using an increased dose of MCE with a low level of total proteolytic activity (Chy-max M) allows achieving a low level of proteolysis during cheese ripening and increasing its shelf life

(E)-2-(hydroxystyryl)-3-phenylquinazolin-4(3H)-ones: Synthesis, photochemical and luminescent properties

The new (E)-2-(hydroxyarilethenyl)-3-phenylquinazolin-4(3H)-ones with various substituents in phenyl fragment were synthesized. The effect of electron donor and acceptor substituents (±M) in quinazolinones on luminescence intensity and dual emission in 550-650-nm wavelength range was shown. The fact of the reversible photo/thermal E-Z isomerization for several substances was established. The (E)-2-(5-chloro-2-hydroxystyryl)-3-phenylquinazolin-4(3H)-one had shown the best combination of photochemical (E-Z isomerization) and photophysical properties. The (E)-2-(2-hydroxy-5-morpholinostyryl)-3-phenylquinazolin-4(3H)-one had revealed the best ESIPT-luminescence (Φrel = 5.3 %). © Arkat. All Rights Reserved.Russian Foundation for Basic Research, RFBR: 18-03-00112This work was supported by the Russian Foundation for Basic Research (grant 18-03-00112). Analytical studies were carried out using equipment of the Center for Joint Use SSpectroscopy and ?nalysis of Organic Compounds ? at the Postosvky Institute of Organic Synthesis of the Russian Academy of Sciences (Ural Branch)

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation

Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components. Current protocols induce myogenic differentiation in vitro through serum starvation, that is, an abrupt reduction in serum concentration. Here we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation, including IGF1R, TFRC and LPAR1. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Moreover, the serum-free differentiation media supported the fabrication of three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production.Bovine satellite cells undergoing myogenic differentiation in a chemically defined, serum-free medium are comparable to those undergoing serum starvation-demonstrating the suitability of this formulation for cultured meat production