CD4⁺PD-1^hi T cells express significantly high levels of CXCR5 and IL-21 mRNA <i>ex vivo</i>.

<p>(a) Representative dot plots showing the gating strategy and sort purity of CD4⁺PD-1^hi and CD4⁺PD-1^– T cells. b) Fold change in expression of CXCR5 and IL-21 mRNA levels in CD4⁺PD-1^hi T cells relative to CD4⁺PD-1^– T cells. mRNA expression was determined <i>ex vivo</i> in sorted cells by relative qRT-PCR.</p

CD4⁺PD-1^hi T cells significantly correlates with CD20⁺Bcl6⁺Ki-67⁺IgG⁺ B cells in LN.

<p>(a) Representative dot plots showing the gating strategy used for analyzing Bcl6 and Ki-67 expression on IgG⁺ B cells in the lymph node (LN) and b) relative proportions of IgG⁺ B cells in LN expressing Bcl6 and Ki-67. Correlation between LN CD4⁺PD-1^hi T cells and c) CD20⁺Bcl6⁺Ki-67⁺IgG⁺ B cells and d) CD20⁺Bcl6^–Ki-67⁺IgG⁺ B cells in LN.</p

Lymph nodes are enriched for CD4⁺PD-1^hi T cells.

<p>(a) Representative dot plot b) Mean fluorescence intensity (MFI) of PD-1 expression on lymph node CD4⁺ and CD4^– T cells subsets. c) Relative proportions of CD4⁺PD-1^hi T cells and CD4^– PD-1^hi T cells in LN and d) relative proportions of CD3 gated CD4⁺PD-1^hi T cells in LN and peripheral blood mononuclear cells (PBMC).</p

CD4⁺PD-1^hi T cells are predominantly CCR7^lo, Bcl6⁺, IOCS⁺ and express a central memory phenotype.

<p>Representative dot plots showing the expression of a) Bcl6 and ICOS on CD3⁺CD4⁺ T cells and b) CD28 and CD95 expression on lymph node (LN) PD-1^hi and PD-1^–CD4 T cell subsets. c) Relative proportions of Bcl6⁺, ICOS+, CD28⁺CD95⁺ CD4⁺PD-1^hi T cells in LN. d) Representative histograms and e) Mean fluorescence intensity (MFI) of CCR7 expression on LN PD-1^–, PD-1^int and PD-1^hiCD4 T cell subsets.</p