Bedeutung der Polysialyltransferasen ST8SiaII und ST8SiaIV in der postnatalen Gehirnentwicklung der Maus

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A systematic and methodical approach for the efficient purification of recombinant protein from silkworm larval hemolymph

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Dependence of GT3-FCHASE extension by purified B-PST on CMP-Neu5Ac concentration

<p><b>Copyright information:</b></p><p>Taken from "Biochemical characterization of a polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases"</p><p></p><p>Molecular Microbiology 2007;65(5):1258-1275.</p><p>Published online Jan 2007</p><p>PMCID:PMC2169525.</p><p>© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd</p> Purified B-polyST was incubated with GT3-FCHASE and CMP-Neu5Ac for 5 min as described in . The respective CMP-Neu5Ac concentrations are indicated. The reaction mixtures were then adjusted to 25% ethanol. The supernatants were applied to a DNA Pac PA-100 column and chromatographed with a gradient of NaNO according to and