output_read2snps_cov4_10_Q30

output data generated by Read2snps tool. This file contain only SNPs sequences with SNPs with a depth sequencing between 4 and 10 for a minimum quality of 30. This file have been used to select and to design primers

assembling_Ixodes_ricinus_RRL

assembling_Ixodes_ricinus_RR

List of studies reporting the presence of <i>Wolbachia pipientis</i> associated with ticks.

<p>List of studies reporting the presence of <i>Wolbachia pipientis</i> associated with ticks.</p

<i>Ixodiphagus hookeri</i> (Hymenoptera : Chalcidoidea, Encyrtidae).

<p>A Female habitus. B Female ovipositing in an engorged nymphs of <i>Ixodes ricinus</i> (ovipositor indicated by the arrow). C Adults of <i>I. hookeri</i> around the dead body of an engorged nymph of <i>I. ricinus</i>. D Emergence hole from which parasitoids exit the dead body of the engorged nymph.</p

Parasitism rates of <i>Ixodes rcinus</i> due to <i>Ixodiphagus hookeri</i> and presence of <i>Wolbachia</i> in the wasps

<p>Parasitism rates of <i>Ixodes rcinus</i> due to <i>Ixodiphagus hookeri</i> and presence of <i>Wolbachia</i> in the wasps</p

Phylogenetic tree of <i>Wolbachia pipientis</i>.

<p>A Neighbor-Joining tree based on the the <i>Ftsz</i> sequence obtained with MEGA5 using the Maximum Composite Likelihood distance. Only bootstrap values greater than 80% are shown. Letters A to F (at the right of the name of the host from which the <i>Wolbachia</i> was sequenced and of its accession number) refer to the <i>Wolbachia</i> supergroups already described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030692#pone.0030692-Casiraghi1" target="_blank">[58]</a>.</p

(A) 2-DE map of OT obtained by performing IEF on a 4–7 linear pH range and SDS-PAGE on a constant 12,5% T in the second dimension, to separate proteins clustered in the single spot shown in Fig 4. (B) Immunoblotting of the gel slab indicated in Panel A.

<p>Arrow points to spot originated from separation and identified as FliD.</p

List of proteins identified under the immunoreactive spot.

<p>List of proteins identified under the immunoreactive spot.</p

Immunoblotting of proteins from SG and OT profiles generated as indicated in Fig 1.

<p>PVDF membranes were incubated with the rabbit polyclonal antibodies anti-FliD of <i>M</i>. <i>mitochondrii</i>, followed by anti-rabbit antibody. The protein spot(s) indicated by an arrow in Panel A (OT pool) was tentatively assigned to FliD. Panel B shows the SG profile in which the hypothetical FliD spot is undetectable.</p

High Master gel, showing qualitative differences between the SG and OT 2-DE master gel patterns (NL, pH 3–10 gradient range).

<p>Labelled in green: spots (n = 170 ± 25) common to both SG and OT. Labelled in red: spots (n = 81) exclusive of SG. Labelled in blue: spots (n = 57) detected solely in OT.</p