Effects of 10 daily doses of ethanol exposure on LPS-induced liver, serum and brain IL-1production

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected 1 hr after LPS injection. LPS significantly increased IL-1protein in liver, serum and brain after 10 days of ethanol administration compared with LPS alone treatment. The results are the means ± SEM of two experiments performed with 6 mice each group. * P < 0.05, ** P < 0.01, compared with the saline control mice. P < 0.05, P < 0.01, compared with LPS-treated mice.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p

Effects of a single dose of ethanol exposure on LPS-induced liver, serum and brain TNFα production

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected at 1 hr post LPS treatment. Analysis of TNFα and MCP-1 protein was conducted by ELISA. (A) LPS and ETOH-LPS rapidly increased liver, serum and brain TNFα protein. (B) MCP-1 protein in liver, serum and brain was increased after LPS treatment alone or combined LPS and ethanol treatments. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p

Ten daily doses of ethanol administration potentiated LPS-induced brain TNFα, MCP-1 and IL-1production that remained elevated at 1 week

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for 10 days and were injected intraperitoneally (i.p.) with LPS at indicated doses 24 hrs after ethanol treatment. Brains were collected at 1 week after LPS injection. The levels of TNFα, MCP-1 and IL-1protein were measured by ELISA. (A) In the10-dose ethanol pre-treated group, brain TNFprotein significantly increased in a LPS dose-dependent manner. (B) Exposure to 10 daily doses of ethanol resulted in a significant increase in brain MCP-1 in a LPS dose-dependent manner. (C) Brain IL-1protein synthesis was also enhanced by ethanol pre-treatment. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls.P < 0.05, P < 0.01, compared with the corresponding LPS-treated group.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p

A single dose of ethanol treatment enhanced LPS-induced IL-1protein in brain and serum, but not in liver

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected at 1 hr after LPS injection. Liver, serum and brain IL-1protein was measured by ELISA. (A) A single ethanol dose increased LPS-induced brain IL-1production. (B) A single dose of ethanol increased LPS-induced IL-1protein in serum. (C) A single dose of ethanol did not show potentiation to LPS-stimulated increase in IL-1protein in liver. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls.P < 0.05, P < 0.01, compared with LPS-treated group.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p

Effects of 10 daily doses of ethanol on IL-10 in brain and liver at 1 week after LPS treatment

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for 10 days and were injected intraperitoneally (i.p.) with LPS at the indicated doses 24 hrs after ethanol treatment. Brains and livers were collected at 1 week after LPS injection. The level of IL-10 protein was measured by ELISA. (A) Ten doses of ethanol decreased brain IL-10 and enhanced LPS-induced decrease in IL-10 in a LPS dose-dependent manner at 1 week after LPS treatment. (B) There was a significant increase in liver IL-10 protein in 10 daily doses of ethanol, LPS (0.3 and 3 mg/kg) and ETOH-LPS groups. P < 0.05, ** P < 0.01, compared with the saline controls.P < 0.05, P < 0.01, compared with the corresponding LPS-treated group.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p

Effects of 10 daily doses of ethanol exposure on LPS-induced liver, serum and brain TNFα and MCP-1 production

Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected 1 hr post LPS treatment. TNFα and MCP-1 protein was determined by ELISA. (A) In 10-day ethanol pre-treated group, LPS significantly increased liver, serum and brain TNFα protein compared with LPS alone group. (B) Exposure to 10 daily doses of ethanol resulted in a significant increase in MCP-1 protein in liver, serum and brain. The results are the means ± SEM of two experiments performed with 6 mice each group. * P < 0.05, ** P < 0.01, compared with the saline controls. P < 0.05, P < 0.01, compared with LPS-treated group.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p