Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected at 1 hr after LPS injection. Liver, serum and brain IL-1protein was measured by ELISA. (A) A single ethanol dose increased LPS-induced brain IL-1production. (B) A single dose of ethanol increased LPS-induced IL-1protein in serum. (C) A single dose of ethanol did not show potentiation to LPS-stimulated increase in IL-1protein in liver. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls.P < 0.05, P < 0.01, compared with LPS-treated group.<p><b>Copyright information:</b></p><p>Taken from "Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment"</p><p>http://www.jneuroinflammation.com/content/5/1/10</p><p>Journal of Neuroinflammation 2008;5():10-10.</p><p>Published online 18 Mar 2008</p><p>PMCID:PMC2373291.</p><p></p
