Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -8

Reporter: 4xMRE from the metallothionein B (MtnB) promoter [] fused to firefly luciferase; reference: tubulin promoter fused to renilla luciferase []. Dpy-30L1 and Dpy-30L2 expression constructs were under the control of the actin promoter. 72 hours after transfection, cells were treated with the indicated concentrations of heavy metals for 24 hours. B) Expression level of green fluorescent protein in transgenic larvae that carry an MtnA-YFP reporter construct. Transgenic flies were allowed to lay eggs on normal food or food supplemented with different heavy metals.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

The core domain of these short proteins is highly conserved among different species

Grey shaded: similar, black shaded: identical aa. Dpy-30 '' core'' indicates the consensus core sequence.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -10

Rotein extract was used for each bandshift reaction. Lanes 1 and 4, bandshift with [P]-labeled MRE consensus oligonucleotide (MRE-s). Lanes 2 and 5, same conditions but also including a 200-fold excess of unlabeled MRE-s competitor oligonucleotide (specific competitor). Lanes 3 and 6, same conditions but with a 200-fold excess of unlabeled Sp1 oligonucleotide (nonspecific competitor). Cells had been treated for 6 hours with medium containing 100 μM zinc sulfate.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -3

Protein extract was used for each bandshift reaction. Lanes 1 and 4, bandshift with [P]-labeled MRE consensus oligonucleotide (MRE-s). Lanes 2 and 5, same conditions but also including a 200-fold excess of unlabeled MRE-s competitor oligonucleotide (specific competitor). Lanes 3 and 6, same conditions but with a 200-fold excess of unlabeled Sp1 oligonucleotide (nonspecific competitor). Cells had been treated for 6 hours with medium containing 100 μM zinc sulfate.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -1

Reporter: 4xMRE from the metallothionein B (MtnB) promoter [] fused to firefly luciferase; reference: tubulin promoter fused to renilla luciferase []. Dpy-30L1 and Dpy-30L2 expression constructs were under the control of the actin promoter. 72 hours after transfection, cells were treated with the indicated concentrations of heavy metals for 24 hours. B) Expression level of green fluorescent protein in transgenic larvae that carry an MtnA-YFP reporter construct. Transgenic flies were allowed to lay eggs on normal food or food supplemented with different heavy metals.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -6

females were allowed to mate with wild type or mutant males, the females were then dissected and checked for a GFP signal in their reproductive system. 30 minutes after mating, 20% of wild type sperm had accumulated in female storage organs. However, mutant sperm remained in the uterus and failed to be transferred to seminal receptacle and spermathecae, the female sperm storage organs. Ov – ovaries; spt – spermatheca; src – seminal receptacle; ut – uterus; ovd – oviduct.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -2

To firefly luciferase; reference, tubulin promoter fused to renilla luciferase. Dpy-30L1 and the mouse ortholog were under the control of the actin promoter. 72 hours after transfection, the medium in half of the plates was supplemented with 40 μM cadmium chloride for 24 hours, while the others served as controls. A) Mouse MTF-1 was co-transfected in the indicated samples; B) MTF-1 was co-transfected in the indicated samples.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p

Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in -5

2A D variant (H2AvD). Arrow, H2AvD-GFP in degradation; arrowheads, H2AvD-GFP degraded. Incorporation of protamine B and Mst77F was analyzed in knockout males that carry either a transgene of protamine B fused to eGFP or of Mst77F fused of eGFP. During the "canoe" and "post-canoe" stages of spermatid development, ProtamineB-eGFP and Mst77F-eGFP incorporation in the spermatid nucleus appeared to be normal in knockout males. Any (diffuse) YFP signal from the promoter was not filtered out.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p