Reporter: 4xMRE from the metallothionein B (MtnB) promoter [] fused to firefly luciferase; reference: tubulin promoter fused to renilla luciferase []. Dpy-30L1 and Dpy-30L2 expression constructs were under the control of the actin promoter. 72 hours after transfection, cells were treated with the indicated concentrations of heavy metals for 24 hours. B) Expression level of green fluorescent protein in transgenic larvae that carry an MtnA-YFP reporter construct. Transgenic flies were allowed to lay eggs on normal food or food supplemented with different heavy metals.<p><b>Copyright information:</b></p><p>Taken from "Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in "</p><p>http://www.biomedcentral.com/1471-213X/8/68</p><p>BMC Developmental Biology 2008;8():68-68.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2478662.</p><p></p
