Heteroleptic Metallosupramolecular Complexes of Bodipy Dyes as Energy Transfer Cassettes

Cataloged from PDF version of article.Bodipy dyes with integral phenanthroline and terpyridine units heteroleptically assemble in the presence of zinc(II) ions to form energy transfer cassettes. These discrete complexes exhibit an approach to modularly designed efficient energy transfer and light harvesting systems through metal ion coordination

Identification of Turkish isolate of the entomopathogenic fungi, Purpureocillium lilacinum (syn: Paecilomyces lilacinus) and its effect on potato pests, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) and Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinumwas found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml-1at 25°C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15°C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved

Identification of Turkish Isolate of the Entomopathogenic Fungi, Purpureocillium lilacinum (syn: Paecilomyces lilacinus) and its Effect on Potato Pests, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) and Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

WOS: 000358899300019Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phihorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinum was found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml(-1) at 25 degrees C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15 degrees C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM.Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK 111O784]A part of this study (preparation of P. lilacinum) was supported by the Scientific and Technical Research Council of Turkey (TUBITAK 111O784). Thanks are due to Drs. F.D. Erdogus and M. Alkan (Dept. of Entomology, Plant Protection Central Research Institute, Ankara-Turkey) for their assisstance throughout this study

Nematicidal Activity of Beauveria bassiana (Bals.-Criv.) Vuill. against Root-Knot Nematodes on Tomato Grown under Natural Conditions

WOS: 000400123900016Plant parasitic nematodes such as root-knot nematodes (RKNs) are a major threat to vegetables, fruits and ornamental plants which cause yield loss. One of the best alternative methods of nematode control is using the entomopathogenic fungi (EPF). The aim of this study was to evaluate the effects of two Turkish isolates of entomopathogenic fungi, Beauveria bassiana (Bals.-Criv.) Vuill. (F-56 and F-63), against naturally infected mixed populations of Meloidogyne incognita and Meloidogyne javanica on tomatoes under natural conditions. The experiment contained 3 conidial suspensions (10(6), 10(7) and 10(8) cfu ml(-1)) with 4 replicates of each treatment. A total of 32 plants were arranged in a randomized block. EPF were applied 4 times (15 days before planting, planting, 15, and 30 days after planting). Nematicide [Paecilomyces lilacinus strain 251 (BioAct (R))] was used as a positive control. At the end of the growing season, the efficiency of the applications was determined according to the root gall index (I) and yields of tomatoes (II). The fungi concentration of 10(8) cfu ml(-1) was significantly greater than in the others. This concentration from both isolates [F-56 (4.59 +/- 0.54) and F-63 (2.77 +/- 0.59)] controlled M. incognita and M. javanica and increased the yield of tomatoes. The results demonstrated the suitability of using B. bassiana isolate F-56 and F-63 for reducing RKNs on tomatoes under greenhouse conditions

Molecular characterization of phytoplasma diseases of pepper in Turkey

During autumn, an extensive survey was conducted in pepper (Capsicum annum L.) in intensive cultivation areas of four provinces in southeastern Turkey (Adana, Kahramanmaraş, Mersin and Şanlıurfa) in order to identify the causal agent (s) of phytoplasma-like symptoms (chlorosis, little-leaf, short internodes and stunting). DNA amplification by PCR and RFLP analysis using EcoRI restriction enzyme confirmed the presence of phytoplasmas in Şanlıurfa and Mersin, and consequently their possible association with the symptoms. Sequencing and phylogenetic analysis revealed that the isolate from Şanlıurfa had 99% sequence identity with “Candidatus Phytoplasma trifolii” (16SrVI) and is a member of the clover proliferation group (16SrVI-A). Additionally, the isolate from Mersin had 96% sequence identity with “Candidatus Phytoplasma asteris” (16SrI). Importantly, gene sequence of the Mersin isolate shared <97.5% similarity to previously discovered “Ca. Phytoplasma” species. Consequently, the phytoplasma detected from Mersin could represent a new “Ca. Phytoplasma” species and to our knowledge, this is the first report of asteris-like phytoplasmas infecting pepper in Turkey. © 2019 Blackwell Verlag Gmb