Disquiet Depictions

This written document is the accompanying thesis for my Master of Fine Arts exhibition - Disquiet Depictions. Presented within the exhibition was a collection of paintings, ranging from acrylic to mixed media. As someone living with both anxiety and panic disorder the symptoms that I have experienced, as well as the coping mechanisms that I have practiced, all influenced the paintings displayed within this exhibition. Imagery included self-portraiture across a variety of emotional expressions felt during moments of sporadic fear and happiness, vibrant geometric patterns serving therapeutic purposes, and subtle medication symbolism. Collectively, these paintings provide a visual journey into the realm of mental health by creating a chronicle of the internal and external disturbances experienced by an individual, only to be alleviated by the act of artmaking. Through observing these works the audience can begin to consider how academic art and therapeutic art co-function in order to convey a personal message across all environments that harbor creative expression, therefore extinguishing the stigma that may come from the public display of distress; if compassion or rejection is projected at the individual on a basis of normality, and if their coping mechanism valid

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field