Studies on the Chronic Consumption of Thermo Oxidized Palm Oil on the Histomorphology of the Cerebellum in Mice

The effect of thermoxidized palm oil (TPO) diet on the histomorphology of cerebrum was studied using mice.  Thirty (30) albino mice weighing between 18-24g and aged 2-4 weeks were grouped into two groups; fifteen (15) animals each group and fed, TPO diet and normal rat chow (control diet) for 14 weeks. Body weight change was significantly lower in the TPO group compared to control (p< 0.01). Histologic micrograph showed hyperchromasia of the granule cell layer of the cerebellum in TPO. From the results, thermoxidised palm oil diet may have adverse effects and degenerative changes in the cerebellum. Keywords: Chronic consumption, Thermo-oxidized palm oil, Cerebellum, Histomorphology

Nicotine Consumption and its Effect on Fecal Composition and Moisture Loss in Albino Rats.

This research assessed nicotine consumption and its effect on fecal composition and moisture loss in albino rats. Thirty albino rats weighing between 80-190g were assigned into 2 groups of (15 each). Group A were fed normal rat chow, group B were fed nicotine diet comprising 5ml of concentrated nicotine dissolved in 200ml of water and mixed with 100g of animal feed. Both groups were fed for 28 days and had free access to drinking water. The results revealed a significant (P<0.001) decrease in water intake in the tobacco fed group when compared to the control with no significant (P>0.05) difference in food intake among the groups. Fecal weight of the tobacco group was not significantly different from the control. Keywords: Nicotine, Fecal, Composition, Moisture loss, Albino rat

Electrolytic Evaluation of Nicotine Treated Rats

This work assessed the effects of electrolyte in rats treated with nicotine diet. A total of thirty (30) rats weighing 80-190g were randomly assigned into two groups of fifteen (15) each. Group A was fed with normal rat chow and acted as the control group while group B received 0.5ml of concentrated nicotine dissolved in 200ml of distilled water mixed with 100g of animal feed. They were fed for 28 days and had free access to drinking water. The results revealed that Sodium (Na+), Potassium (K+) and Chloride (Cl-) were significantly higher (P<0.001) when compared with the control group, while the bio-carbonate (HCO3-) was lower. This result is suggestive that nicotine consumption is capable of changing the ionic composition of the body. Key words: Wistar rat, Nicotine, Electrolyti

Some Histological Changes in the Intestines of Alloxan Induced Diabetic Mellitus Albino Rats

Changes in intestinal histology of the Albino rats with alloxan induced diabetes mellitus was investigated using fifteen (15) albino rats. The rats were divided into 3 groups A, B, C. Group A served as the control group, group B served as the experimental low dose treated with 100mg/bw of Alloxan, while group C served as the experimental high dose was treated with 200mg/bw of Alloxan. The small intestines were removed and passed through routine tissue processing. The results in the rat of the control group A showed the mucosa, sub-mucosa, muscle and serious layer, luminar surfaces of the villi were lined by simple columnar cells. The brunner glands were also seen in the submucosa. While in rat of group B (low dose); the brunner glands appear to have decreased in size and number of goblet cells appeared more columnar. The histology of the layer was almost as similar to that of the control group. Group C (high dose); whereas in rats in group C was degeneration of the brunner glands characterized by pale staining cytoplasm; the nuclei of the columnar cells of the villi appear pykonotic; there was also loss of villi; the sub-mucosa coat appears to have undergone fibrotic changes; and, the various layers appear indistinct. Our results suggest that high dose of alloxan causes degeneration of the various layers of the duodenum. Keywords: Alloxan, Diabetes mellitus, Wistar rats, Histological change

The Electrolytic Effect of Sida Acuta Leaf Extract on the Kidney Electrolyte of Adult Wistar Rats

This study was carried out to assess the effect of ethanolic leaf extract of Sida acuta on the kidney electrolytes of adult Wistar rats. Forty five rats weighing between 140-180g were assigned to three groups (A, B and C) with fifteen animals each. Group A served as the control while groups B and C served as the experimental groups and received 100mg/kgbw and 200mg/kgbw of the extract respectively for fourteen days. All the animals were sacrificed after  fourteen days. Blood was collected by cardiac puncture for biochemical analysis of serum electrolytes. Serum chemistry revealed significantly raised sodium and decreased potassium levels in animals treated with 100mg/kgbw, but chloride, creatinine and potassium not significantly affected (P>0.05). Animals treated with 200mg/kgbw of the extract had significantly raised sodium levels, but reduced potassium, chloride and creatinine relative to control (P<0.05). From the result of this experiment, it is concluded that the administration of ethanolic extract of Sida acuta leaves could have adverse effects on the kidney electrolytes of Adult Wistar rats at the doses and duration used in the course of this experimentation Keywords – Biochemical, Electrolyte, Kidney, Serum, Sida acut

Ovarian profile of Wistar rats treated with Theobroa cacao extract.

The study was aimed at determining the effect of aqueous extract of Theobroma cacao on the histology of the ovary of female albino wistar rat. Twenty-four (24) adult wistar female rats weighting about 100- 160g were used for this research work and were divided into three (3) groups of eight (8) animals each. Group A; control, Group B; low dose and Group C; high dose with eight (8) animals in each group. Control group received vital feed; the low dose group was administered 240mgkg body weight of Theobroma cacao extract and the high dose group was administered 500mgkg body weight of the test substance. Extract was given daily by oral gavage method for twenty-one (21) days. Twenty-four hours after the last administration, all animals in each group were sacrificed under chloroform anesthesia. The ovaries were harvested, weighed and fixed in 10% buffered formalin for histological studies. Results showed that following administration of extract of Theoboma cacao at these doses, an insignificant decrease in organ weight was observed. Histological observation showed few follicles as well as loss of the substance of granulose cell this could possibly suggest decrease in production of sex steroids in the ovary.Keywords: Theobroma Cacao, Ovary, Histolog

Accessment of the Kidney in Ginger Treated Wistar Rats

Ginger, botanically known as Zingerber offinale is an underground stem or rhizome which is known to have originated from Asia and have been reported to have a number of medicinal properties which is used in the treatment of many ailments such as arthritis, painful menstrual periods, nausea etc. It is also used as a common specie in food and bakery industries. The effect of ethanolic extract of ginger on the kidney of male wistar rats was studied due to the availability and medicinal uses of ginger. Twenty five (25) adult Wistar rats weighing 125-200g were divided into five groups. Group A animals served as the control and were served with distilled water , B served as vehicle control groups and received olive oil.  The animals in groups C, D and E served as experimental groups, and received 100mg/kg, 250mg/kg and 500mg/kg body weight of the ethanolic extract of Zingiber officinale respectively. The animals received the extract for 14 days and were sacrificed 24 hours after the last administration and the kidney was routinely processed histologically. The study shows enlargement of Bowman’s space, disintegration of glomerular cells and dilation of the tubular lumen with shrinkage of tubular luminal cells when compared to the control group, which progressively increased as the duration of intake of Zinger officinlae increased. Therefore, Zingerber officinale should be used with caution because it may have deleterious effects on the liver cells at high doses. Keywords: Assessment, Ginger, Treatment, Kidney, Wistar ra

Extract of Sesamum Indicum Seeds on the Glycogen Profile of the Liver of Adult Wistar Rat

The aim of this work was to evaluate the effect of seeds extract of Sesamum indicum on the histology liver of adult Wistar rats. Thirty Wistar rats weighing between 150-180g were divided into three groups of ten each; control group A received distilled water, experimental B and C received 200mg/kg and 400mg/kg of the seed extracts respectively. The extracts were administered for two weeks, at the end of which the animals were sacrificed; livers were removed and processed for Haematoxylin and eosin (H&E) paraffin sectioning and staining method while the serum was used for liver enzyme assay. From the results obtained, the administration of ethanolic seed extract of Sesamum indicum caused no obvious structural derangement in the organ There were no adverse effects on glycogen distribution. Keywords: Liver, Wistar rats, Sesamum indicum, glycoge

Extract of Ginger (Zingiber Officinale) on the Histology of the Spleen Using Adult Male Rats

Usage of botanical medicine is ancient and plant chemicals are still the backbone of our pharmacopoeia because more than 50% of drugs used in Western pharmacopoeia are isolated from herbs or derived from modification of chemicals first found in plants. Zingiber officinale is one of the most widely used herbs and food flavouring agent and commonly known as ginger. This study was done to evaluate the possible histological effect(s) of ethanolic extract of Zingiber offinale on the liver of adult male albino rats. Twenty five (25) adult wistar rats weighing between 125-200g were divided into five groups of five (5) rats each. Group C, D and E served as experimental groups while group A and B served as the control groups. Group C was administered a dose of 100mg/kg of the extract, group D was given oral dose of 250mg/kg of the extract while group E was given oral dose of 500mg/kg of Zingiber officinale extract. Administration of extract lasted for fourteen days at the end of which the animals were sacrificed using chloroform-inhalation method. The spleen was harvested as tissue samples from sacrificed animals for pathological examination using routine histological procedure and stained with the haematoxylin and eosin stains. Histological examinations of spleen showed that after treatment with low and medium doses (100, 250mg/kg) the ginger extract produced little damaging effects on the spleen histology, indicating moderate lymphoid hyperplasia in the splenic cells. But at higher dosage (500mg/kg), reactive lymphoid hyperplasia in the splenic cells was extensive. Therefore, Zingerber officinale should be used with caution because it may have harmful effects on the spleen cells at high doses. Keywords: Ginger, Histology, Ethanolic extract, Spleen, Wistar rat