47 research outputs found
POTENSI KOMBINASI EKSTRAK AKAR SIDAGURI (Sida rhombifolia L.) DAN GETAH JARAK (Jatropha curcas L.) SEBAGAI BAHAN DEVITALISASI
Get PDFBackground: Arsenic paste was used to devitalize dental pulp in some problems before surgical, but it has been proven to have many hazardous effects. So, other alternative drugs are needed that are equally effective but safer. Jatropha (Jatropha curcas L.) can cause lysis blood vessels in the pulp, whereas the sidaguri (Sidarhombifolia L.) are effective in relieving inflammation. The purpose of this study was to determine the devitalization effect of jatropha and sidaguri on rabbit animal model.Method: Sidaguri roots were extracted using reflux method while jatropha sap was dried using lyophilization method. For easy application, the extract made into a paste (1; 1) and inserted into the cavity. After 7 days of exposure, the teeth were removed, calcified and followed to histopathological staining and COX-2 expression (IHC).Result: Histopathological examination showed that the paste was able to cause necrosis of pulp nerve. Higher dose of the drug increased the necrosis area, but the paste is not able to reduce COX-2 expression.Conclusion: It can be concluded that the paste has a potential to developed as devitalization agent although it is necessary to study to the reduce the inflammation process
THE INCREASE OF TOOTH ENAMEL SURFACE HARDNESS AFTER APPLICATION BLOOD COCKLE SHELLS (ANADARA GRANOSA) PASTE AS REMINERALIZATION AGENT
Get PDFObjective: To determine the increase of tooth enamel surface hardness after application hydroxyapatite paste that was synthesized from blood cockle shells (Anadara granosa) as a remineralization agent.
Methods: Laboratory experimental study using twenty-seven maxillary first premolar and randomly divided into 3 groups. All of the samples were immersed in the non-cola carbonated drink (2 min). Thereafter, samples in each group were treated (6 min) with application of blood cockle shells paste that has been synthesized (group 1), casein phosphopeptide-amorphous calcium phosphate paste (GC Tooth Mousse®) (group 2) as a positive control, and stored in saline solution (NaCl) (group 3) as a negative control. Vickers Hardness Number (VHN) measurement was performed at baseline, after immersing in non-cola carbonated drink and after completing of the respective treatment.
Results: Immersion in non-cola carbonated drink reduced the enamel surface hardness significantly. Significant re-hardening after treated occurred in group 1 and 2 also baseline hardness of both groups were achieved. But statistically no significant differences between group 1 and 2 in re-hardening enamel surface hardness (final hardness-hardness after immersion).
Conclusion: Application of blood cockle shells paste as a remineralization agent could increase tooth enamel surface hardness which is nearly the same effective as CPP-ACP paste
Viabilities of Odontoblast Cells Following Addition of Haruan Fish in Calcium Hydroxide
Get PDFBackground: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing and anti-inflammation. This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. Materials and Methods: Firstly, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by MTT assay on 24 h after the last treatment. While cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 µg/mL). Untreated cells were used as control. Result: Adding of HFE at range 25.0 till 100 µg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p<0.05). Conclusion: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent.
BACKGROUND: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing, and anti-inflammation.
AIM: This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2.
MATERIALS AND METHODS: First, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay on 24 h after the last treatment, while cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 μg/mL). Untreated cells were used as control.
RESULTS: Adding of HFE at range 25.0 until 100 μg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p < 0.05).
CONCLUSION: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent
Aktifitas antiinflamasi dan analogetik akar sidaguri (s.rhombifolia)
No full textLatar belakang: penyakit pulpa dan periapikal menurut profil data kesehatan indonesia tahun 2009,berada pada urutan ke-8 dari 1o besar pasien rawat jalan di rumah sakit.keadaan ini terus meningkat, dimana pada data tahun 2010 penyakit pulpa dan periaplikal naik ke-7 sebagai 10 besar pasien rawat jalan di rumah sakitxiv,42 hlm
