Additional file 1 of Breast cancer screening and early diagnosis in China: a systematic review and meta-analysis on 10.72 million women

Additional file 1: Table S1. The summary of detailed search keywords. Table S2. PRISMA checklist. Table S3. The pooled breast cancer detection rates in different subgroups of organized screening programs (China, 2010-2023). Table S4. The pooled early-stage (0–II) breast cancer detection rates in different subgroups of organized screening programs (China, 2010-2023). Table S5. The pooled proportion of early-stage (0–II) breast cancer in different subgroups of organized screening programs (China, 2010-2023). Figure S1. Forest plot of pooled breast cancer detection rate (China, 2010-2023) (A) opportunistic screening; (B) population screening. Figure S2. Forest plot of pooled early-stage (0–II) cancer detection rate (China, 2010-2023) (A) opportunistic screening; (B) population screening. Figure S3. Forest plot of pooled the proportion of early-stage (0–II) cancer (China, 2010-2023) (A) opportunistic screening; (B) organized screening

DataSheet_1_Analysis of the key ligand receptor CADM1_CADM1 in the regulation of thyroid cancer based on scRNA-seq and bulk RNA-seq data.zip

IntroductionAdvanced papillary thyroid cancer (PTC) has a poor prognosis, 60~70% of which become radio iodine refractory (RAI-R), but the molecular markers that assess PTC progress to advanced PTC remain unclear. Meanwhile, current targeted therapies are badly effective due to drug resistance and adverse side effects. Ligand-receptor pairs (L/R pairs) play an important role in the interactions between tumor cells and other cells in the tumor microenvironment (TME). Nowadays, therapies targeting ligand-receptor pairs in the TME are advancing rapidly in the treatment of advanced cancers. However, therapies targeting L/R pairs applied to advanced PTC remains challenging because of limited knowledge about L/R pairs in PTC.MethodsWe screened the critical L/R pair: CADM1-CADM1 using 65311 single-cell RNA sequencing (scRNA-seq) samples from 7 patients in different stage of PTC and bulk RNA-seq datasets containing data from 487 tumor samples and 58 para-carcinoma samples. Moreover, the expression levels of CADM1-CADM1 was assessed by quantitative real time polymerase chain reaction (qRT-PCR) and the function was analyzed using Transwell immigration assay.ResultsWe found that CADM1_CADM1 could be regarded as a biomarker representing a good prognosis of PTC. In addition, the high expression of CADM1_CADM1 can strongly increase the sensitivity of many targeted drugs, which can alleviate drug resistance. And the results of qRT-PCR showed us that the expression of CADM1_CADM1 in PTC was down-regulated and overexpression of CADM1 could suppresses tumor cell invasion migration.ConclusionOur study identified that CADM1_CADM1 played an essential role in the progression of PTC for the first time and our findings provide a new potential prognostic and therapeutic ligand-receptor pair for advanced PTC.</p

Construction of Uranyl Organic Hybrids by Phosphonate and in Situ Generated Carboxyphosphonate Ligands

The hydrothermal reaction of uranyl ions with (5-methyl-1,3-phenylene)­diphosphonic acid (H₄MPDP) in the presence of additives such as nitric acid, N-bearing species, and heterometal ions yielded five new uranyl organic hybrids: (H₃O)­[(UO₂)₅(H₂O)₄(H₃DPB)₂(H₂DPB)­(HDPB)]·2H₂O (<b>1</b>), (Hphen)­(phen)­[(UO₂)₃(H₂DPB)­(HDPB)] (<b>2</b>), (H₂dipy)­[(UO₂)₃(MPDP)₂] (<b>3</b>), Zn­(bipy)­(UO₂)­(MPDP) (<b>4</b>), and Co­(bipy)­(UO₂)­(MPDP)·H₂O (<b>5</b>) (H₅DPB = 3,5-diphosphonobenzoic acid; phen = 1,10-phenanthroline; dipy = 4,4′-bipyridine; bipy = 2,2′-bipyridine). Single-crystal X-ray diffraction (XRD) demonstrates that <b>1</b> and <b>2</b> are 3D frameworks constructed of uranyl centers and carboxyphosphonate DPB ligands; the latter were formed via the in situ oxidation of H₄MPDP. In the homometallic uranyl diphosphonate <b>3</b>, less common UO₆ square bipyramids connected by MPDP ligands were incorporated to form the 2D assembly. A further introduction of heterometal ions produced two heterobimetallic uranyl phosphonates <b>4</b> and <b>5</b>. Both of them show layered structures, formed by UO₆ square bipyramids linked by MPDP ligands with heterometal-centered polyhedra decorated on the sides of the layers. It is found that the pH and heterometal ions have significant effects on the structures of the complexes. In addition to the syntheses and XRD characterization, the spectroscopic properties of these uranyl complexes were also addressed. To complement the experimental results, density functional theory calculations were carried out on several model complexes that feature a homo- or heterobimetallic molecular skeleton. Geometrical/electronic structures, IR spectra, and electronic absorptions were discussed

Construction of Uranyl Organic Hybrids by Phosphonate and in Situ Generated Carboxyphosphonate Ligands

The hydrothermal reaction of uranyl ions with (5-methyl-1,3-phenylene)­diphosphonic acid (H₄MPDP) in the presence of additives such as nitric acid, N-bearing species, and heterometal ions yielded five new uranyl organic hybrids: (H₃O)­[(UO₂)₅(H₂O)₄(H₃DPB)₂(H₂DPB)­(HDPB)]·2H₂O (<b>1</b>), (Hphen)­(phen)­[(UO₂)₃(H₂DPB)­(HDPB)] (<b>2</b>), (H₂dipy)­[(UO₂)₃(MPDP)₂] (<b>3</b>), Zn­(bipy)­(UO₂)­(MPDP) (<b>4</b>), and Co­(bipy)­(UO₂)­(MPDP)·H₂O (<b>5</b>) (H₅DPB = 3,5-diphosphonobenzoic acid; phen = 1,10-phenanthroline; dipy = 4,4′-bipyridine; bipy = 2,2′-bipyridine). Single-crystal X-ray diffraction (XRD) demonstrates that <b>1</b> and <b>2</b> are 3D frameworks constructed of uranyl centers and carboxyphosphonate DPB ligands; the latter were formed via the in situ oxidation of H₄MPDP. In the homometallic uranyl diphosphonate <b>3</b>, less common UO₆ square bipyramids connected by MPDP ligands were incorporated to form the 2D assembly. A further introduction of heterometal ions produced two heterobimetallic uranyl phosphonates <b>4</b> and <b>5</b>. Both of them show layered structures, formed by UO₆ square bipyramids linked by MPDP ligands with heterometal-centered polyhedra decorated on the sides of the layers. It is found that the pH and heterometal ions have significant effects on the structures of the complexes. In addition to the syntheses and XRD characterization, the spectroscopic properties of these uranyl complexes were also addressed. To complement the experimental results, density functional theory calculations were carried out on several model complexes that feature a homo- or heterobimetallic molecular skeleton. Geometrical/electronic structures, IR spectra, and electronic absorptions were discussed

Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats

<div><p>Transplantation of testicular cells and tissues has been studied for the investigation of immunology of the testis, which is an immunologically privileged organ. However, reports of transplant of the testis at organ level have been extremely limited because of technical difficulties of the orthotopic testis transplantation (OTT) in experimental animals. In the present study, we developed a new and simple model of the heterotopic testis transplantation (HTT), which is donor testis transplantation into the cervical region of recipients, in a syngeneic model in rats [donor Lewis (LEW) graft to LEW recipient]. The duration of HTT was significantly shorter and success rate higher than that of OTT. To histologically evaluate HTT, the local immune responses were compared among the syngeneic model, an acute rejection allogeneic model [donor Augustus Copenhagen Irish (ACI) graft to LEW recipient] and a chronic rejection allogeneic model (donor F344 graft to LEW recipient) at postoperative day 3. We found that allogeneic ACI grafts resulted in mild and not severe orchitic lesions, whereas immune responses of allogeneic F344 grafts seemed intact and were not significantly different from those of syngeneic LEW grafts. These results suggest that our new operative procedure will be useful in future for the investigation of the testicular immunology.</p></div

mRNA levels in the grafted testes detected by real time RT-PCR.

<p>In the ACI group, mRNA expression levels of inflammatory mediators, such as IFN-γ, IL-1β, and IL-10, and apoptosis-related genes, such as caspase 3 and caspase 8, were significantly higher than in the LEW group (n = 3 for each group). *P < 0.05 vs. LEW group. **P < 0.01 vs. LEW group.</p