Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-7

Hanges. Phosphate-buffered saline-injected mice showed minimal signs of inflammation. Staining for macrophages was strongly positive. CD3-positive T cells were also present. Staining specificity was confirmed using, as primary antibody, nonimmune isotype-matched antibodies. Fibrin deposition was assessed by fibrin immunohistochemistry.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-4

*= 0.001, Wilcoxon rank sum test. WT, wildtype; KO, knockout.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-5

Reatment given 16 hours prior to soluble tissue factor injection. Platelet counts were performed 40 hours after injection of antibody. Footpad swelling was greatly reduced in mice treated with antiplatelet antibody. Antiplatelet antibody-treated mice, n = 10; control, rabbit normal serum-treated mice, n = 10. *< 0.05, **< 0.01, test.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-3

Ur different protease activated receptors (PARs). PAR-1-deficient mice. PAR-2-deficient mice. PAR-3-deficient mice. PAR-4-deficient mice. In each experiment, footpad swelling was assessed in the PAR-deficient mice and their littermates (+/+ or +/-) as controls.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-2

or untreated mice (n = 7), were injected with 1 μg soluble tissue factor. Results from all treated groups were significantly reduced (< 0.05 by test) compared with the control group. Wildtype mice were treated with ancrod (n = 7) or with phosphate-buffered saline (n = 7) and then injected with 1 μg soluble tissue factor. Results from ancrod-treated mice are significantly different from the control group, at all time points (< 0.05 by test).<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-0

) were detected using a polyclonal rabbit anti human TF antibody. Effect of sTF injected into the footpad: 1 μg sTF (in 10 μl phosphate-buffered saline) was injected into the intraplantar region of the right hindfootpad. The contralateral footpad was injected with the same volume of phosphate-buffered saline. Swelling was observed in the sTF-injected footpad after 2 hours and was sustained over 24 hours (photograph). Dose-dependent effect of sTF-induced inflammation: 0.2 to 5 μg sTF in 10 μl was administered into the hindfootpad. The contralateral footpad was injected with phosphate-buffered saline. Results expressed as the percentage increase in the right over left footpad thickness. *< 0.05, **< 0.01 and ***< 0.001, Wilcoxon rank sum test.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-1

Hanges. Phosphate-buffered saline-injected mice showed minimal signs of inflammation. Staining for macrophages was strongly positive. CD3-positive T cells were also present. Staining specificity was confirmed using, as primary antibody, nonimmune isotype-matched antibodies. Fibrin deposition was assessed by fibrin immunohistochemistry.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p

Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation-6

) were detected using a polyclonal rabbit anti human TF antibody. Effect of sTF injected into the footpad: 1 μg sTF (in 10 μl phosphate-buffered saline) was injected into the intraplantar region of the right hindfootpad. The contralateral footpad was injected with the same volume of phosphate-buffered saline. Swelling was observed in the sTF-injected footpad after 2 hours and was sustained over 24 hours (photograph). Dose-dependent effect of sTF-induced inflammation: 0.2 to 5 μg sTF in 10 μl was administered into the hindfootpad. The contralateral footpad was injected with phosphate-buffered saline. Results expressed as the percentage increase in the right over left footpad thickness. *< 0.05, **< 0.01 and ***< 0.001, Wilcoxon rank sum test.<p><b>Copyright information:</b></p><p>Taken from "Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation"</p><p>http://arthritis-research.com/content/10/2/R42</p><p>Arthritis Research & Therapy 2008;10(2):R42-R42.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2453761.</p><p></p