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Water Permeability and Elastic Properties of an Archaea Inspired Lipid Synthesized by Click Chemistry
Water Permeability
and Elastic Properties of an Archaea
Inspired Lipid Synthesized by Click Chemistr
Single-Step Homogeneous Immunoassays Utilizing Epitope-Tagged Gold Nanoparticles: On the Mechanism, Feasibility, and Limitations
A single-step gold nanoparticle (AuNP)-based
immunoassay is demonstrated
in which the nanoparticle surface is tagged with short viral peptide
epitopes. Antiviral antibodies with monoclonal specificity trigger
nanoparticle aggregation yielding a colorimetric response that enables
detection of antibodies in the low-nanomolar range within a few minutes. <i>In silico</i> insights into the interactions at the epitope–gold
interface demonstrate that the conformational landscape exhibited
by the epitopes is strongly influenced by the amino acid sequence
and location of particular residues within the peptides. The conformation,
orientation, and linker chemistry of the peptides affect the immune
complex formation in nonintuitive ways that are, nevertheless, explained
by a unique sterically kinetically driven aggregation mechanism. The
rapid and specific performance of the AuNP immunoassay may have generic
potential in point of care diagnostics and other laboratory routines