Developing a Sustainable Freight Transportation Framework with the Consideration of Improving Safety and Minimizing Carbon Emissions

Despite the difficulties of the American economy in recent years the transportation sector continues to expand. Freight transportation alone has been projected to increase enormously even if the economy as a whole only manages a very moderate growth. Not only does freight transportation use a large percentage of resources but it contributes significantly to America’s share of carbon emissions and affects the safety of the transportation system and all its users. These problems are only expected to increase as the volume of freight transportation is already reaching the limit of the American transportation infrastructure’s capacity and demand continues to increase. The primary objective of this research was to compile a list of technologies and practices that should be included in the sustainable freight transportation frameworks of government agencies and commercial fleets to reduce their carbon footprint and increase their safety by providing recommendations on promising legislation, research, technologies, and practices. Data was gathered through a literature review of available materials and a survey of the state Departments of Transportation. The success of this research project provides the needed knowledge for the development of a sustainable freight transportation framework

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Developing a Sustainable Freight Transportation Framework with the Consideration of Improving Safety and Minimizing Carbon Emissions

Despite the difficulties of the American economy in recent years the transportation sector continues to expand. Freight transportation alone has been projected to increase enormously even if the economy as a whole only manages a very moderate growth. Not only does freight transportation use a large percentage of resources but it contributes significantly to America’s share of carbon emissions and affects the safety of the transportation system and all its users. These problems are only expected to increase as the volume of freight transportation is already reaching the limit of the American transportation infrastructure’s capacity and demand continues to increase. The primary objective of this research was to compile a list of technologies and practices that should be included in the sustainable freight transportation frameworks of government agencies and commercial fleets to reduce their carbon footprint and increase their safety by providing recommendations on promising legislation, research, technologies, and practices. Data was gathered through a literature review of available materials and a survey of the state Departments of Transportation. The success of this research project provides the needed knowledge for the development of a sustainable freight transportation framework

Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly

For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae , that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type–related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution