PORK QUALITY OF PIETREN AND HAMPSHIRE CROSSBREEDS

Proizvođači svinjetine proteklih godina imaju značajne probleme u plasmanu svinjetine, između ostalog i s obzirom na učestalu pojavu blijedog, mekanog i vodnjikavog mesa (BMV) i zbog toga što se sve više traže, i više plaćaju, tovljenici bolje kakvoće mišićnoga tkiva za tzv. specijalnu namjenu (pršut, kulen, specijalne kobasice). Cilj ovog istraživanja je uvođenjem novoga genotipa (HPxPI) po očevoj strani, poboljšati kakvoću mišićnoga tkiva novostvorenih križanaca. Istraživanje je provedeno na pet obiteljskih gospodarstava s područja Koprivničko križevačke županije. U klaonicama je ispitana kakvoća mišićnoga tkiva (n=200) križanaca, s majčine strane ŠLxVJ, očeva HPxPI, žive mase 100 kg, mjerenjem pH1, pH2 vrijednosti i boje (L*,a*,b*) u m. gracilis. U ukupnom uzorku (n=200) meso poželjne kakvoće prema pH1 vrijednosti imalo je 87% polovica, odnosno 84% polovica prema pH2 vrijednosti. Pojava BMV mesa zabilježena je prema pH1 u 10% polovica, odnosno 14% polovica prema pH2 u ukupnom uzorku. Prosječne utvrđene vrijednosti boje mesa u ukupnom uzorku bile su L*42,92, a*19,37, b*5,08. Najbolju vrijednost boje imale su polovice petog gospodarstva (L*42,56, a*19,43, b*4,74). Najbolja kakvoća polovica, prema (S)EURO standardu, bila je na prvom gospodarstvu sa 22,50% polovica S klase i 52,50% polovica E klase.In the last few years producers of pork have had great problems with distribution of pork, related primarily to frequent appearance of pale, soft, exudative meat (PSE). The reason is that the market prefers and pays more money for high quality meat, especially for special sausages and ham. The aim of this research is to introduce new father\u27s genotype (HPxPI) to improve meat quality of newly formed crossbreeds. The research included five family farms in Koprivniko-križevaka county. Meat quality pH1, pH2 value and colour (L*,a*,b*) were tested in the slaughterhouse on n=200 crossbreeds (live weight 100kg) from mother\u27s side SLxLW, father\u27s side HPxPI in m. gracilis. In all samples 87% of carcasses had desirable quality according to pH1 value while according pH2 value only 84% carcasses were of desirable quality. Of all samples, only 10% of carcasses according to pH1 value and 14% according to pH2 value had pale, soft, exudative meat (PSE). Average value in all samples was L*42,92, a*19,37, b*5,08 and the best results were found in the fifth family farm (L*42,56, a*19,43, b*4,74). The best carcass quality, according to (S)EURO standard, was in the first family farm with 22,50% of carcasses of S class and 52,50% of carcasses of E class

Incidence of antibiotics and other antibacterial substances in milk collected for the market

Obavljeno je istraživanje učestalosti pojave antibiotika i drugih anti-bakterijskih tvari u mlijeku isporučenom za tržište. Ispitivanja su obavljena u Središnjem laboratoriju za kontrolu mlijeka Hrvatskog stočarskog centra tijekom sedmomjesečnoga razdoblja na ukupno 107.840 uzoraka, odnosno na 15.405 uzoraka u prosjeku mjesečno koji su uzimani na sabirnim mjestima iz rashladnih uređaja. Cilj je bio ukazati na učestalost pojave antibiotika i drugih antibakterijskih tvari u mlijeku, te na neophodnost primjene sveukupnoga menadžmenta kakvoće u kontekstu sprječavanja pojave antibiotika u svježem mlijeku s farmi s obzirom na važnost koju zauzimaju mlijeko i mliječni proizvodi u prehrani ljudi. Za njihovo određivanje u mlijeku korišten je standardni difuzni test (Delvotest MCS, proizvođača DSM Food Specialties, The Netherlands), osjetljiv na antibakterijske tvari kao što su antibiotici, sulfa- ostaci i ostale tvari npr. dezinficijensi, deterdženti itd. Uzorci u kojima je registrirana prisutnost rezidua upućeni su na brzi test koji za manje od sedam minuta otkriva širok raspon beta laktamskih ostataka (Delvo-X-PRESS® βL-II test, istoga proizvođača). Na temelju dobivenih i obrađenih rezultata utvrđeno je da postoje značajne razlike (P<0,05 i P<0,01) između ukupno registriranih rezidua, antibiotika i drugih antibakterijskih tvari u mlijeku. Dobiveni rezultati pokazuju relativno jaku pozitivnu linearnu korelacijsku vezu između prosječnoga broja uzoraka po mjesecima pozitivnih na antibiotike, u odnosu na broj registriranih uzoraka s ukupnim reziduima (r=0,77). Na osnovi rezultata provedenoga istraživanja ne može se izvesti zaključak o većoj ili manjoj prisutnosti antibiotika u kravljemu mlijeku ovisno o godišnjem dobu; jesen, zima, rano proljeće, a zadaću sveukupnoga menadžmenta kakvoće treba i nadalje temeljiti na sprječavanju pojave antibiotika u svježem mlijeku dovezenog s farmi.Incidence of antibiotics and other antibacterial substances in milk collected for the market was studied. Seven months long investigations were conducted at the Central Laboratory for Milk Control of the Croatian Livestock Centre and involved 107.840 samples, or an average of 15.405 samples per month. Samples were taken from the lactofreeze at milk collection centres. The research goal was to determine the incidence of antibiotics and other antibacterial substances in milk, and point to the need of applying integral quality management for prevention of the presence of antibiotics in fresh farm milk in the light of the importance of milk and milk products in human nutrition. The said substances were detected in milk using the standard diffusion test (Delvotest MCS, DSM Food Specialties, The Netherlands), sensitive to antibacterial substances such as antibiotics, sulpha-residues and other substances, e.g. disinfectants, detergents, etc. Samples in which the presence of residues was determined were submitted to a quick test which in less than seven minutes detects a wide range of beta-lactam residues (Delvo-X-PRESS® βL-II test of the same manufacturer). Based on the obtained and analyzed results, significant differences at both levels of significance (P<0.01; P<0.05) were determined between the total detected residues, antibiotics and other antibacterial substances in milk. The results point to a relatively strong linear correlation between the average number of samples per month positive to antibiotics and the number of registered samples with total residues (r=0.77). Research results do not allow the conclusion on higher or lower levels of antibiotics in cow milk in dependence on the season; autumn, winter, early spring. Integral quality management should continue to be based on prevention of the presence of antibiotics in fresh milk delivered from farms

SEMI-CONCENTRATE RATIONS IN HEREFORD BULLOCKS FATTENING

Provedeno je istraživanje utjecaja polukoncentratnih obroka na prirast, utrošak energije i proteina, te na cijenu dnevnoga obroka u tovu muške junadi hereford pasmine. Dvogodišnje istraživanje je provedeno na dvije skupine junadi u govedarskom praktikumu Visokoga gospodarskoga učilišta u Križevcima. Junad je potjecala iz vlastite proizvodnje, u kojoj krave služe za proizvodnju teladi koja je uz kravu do starosti 6-7 mjeseci kada se odbija i stavlja u tov. U pokusnom tovu junadi korištena su četiri polukoncentratna obroka uz maksimalno korištenje voluminozne krme u formi kukuruzne silaže kompletne kukuruzne biljke. Tov junadi počeo je s prosječnom ulaznom tjelesnom masom od 239 kg u prvoj i 247 kg u drugoj skupini (p>0,05), a završio kod prve skupine s 490 kg a kod druge skupine s 510 kg (p0,05), a iznosile su za prvu skupinu 72,15 MJ metaboličke energije i 901,70 g iskoristivog sirovog proteina i za drugu skupinu 67,75 MJ metaboličke energije i 847,50 g iskoristivog sirovog proteina. Ostvarene cijene koštanja dnevnih obroka izdiferencirale su se po kontrolnim razdobljima i rasle su s povećanjem težine junadi, kod prve skupine od 4,88 do 7,38 kn, odnosno kod druge skupine od 5,61 do 8,57 kuna po dnevnom obroku. Ostvarene prosječne razlike u cijeni koštanja dnevnih obroka između dvije skupine junadi su značajne (p0.05) and ended with 490 kg in the first group and 510 kg (p0.05) and was amounted in the first group 72.15 MJ ME and 901.70 g UCP and in the second group 67.75 MJ ME and 847.50 g UCP. Cost of daily ration varied per control periods and grew with the increasing weight of bullocks. In the first group cost per daily ration was from 4.88 to 7.38 kn and in the second group from 5.61 to 8.57 kn. Realized differences of average cost of daily ration between the two groups of bullocks were significant (p<0.05). That may be due to the replacement of part of the protein with nonprotein nitrogen and better quality of corn silage in the rations for the first group of bullocks (390 g DM, 51 g UCP, 4, 26 MJ ME / kg) compared to the second group (ST 343 g, 44 g of UCP, 3.70 MJ ME / kg), which was 69% in the structure of the dry matter of the ration

SECOND FODDER INTERCROP IN BEEF AND MILK PRODUCTION, SUNFLOWER (Helianthus annuss) AND HYBRID SORGHUM (Sorghum sp.)

Provedeno je istraživanje povećanja proizvodnje krme na postojećim obradivim površinama Visokoga gospodarskoga učilišta u Križevcima, uvođenjem u plodored suncokreta (Helianthus annuss) i hibridnoga sirka (Sorghum sp., „Grazer“), kao postrnih međuusjeva. Postrna sjetva svake kulture izvršena je na četiri parcele, pojedinačne veličine oko 1000 m2. Pretkultura je bila pšenica, a predsjetvena gnojidba obavljena je gnojevkom s oko 85 m3/1 ha. Tla sa svih pokusnih parcela mogu se uvrstiti u kisela, dosta humozna tla (humus 3,88%; pH u 1M KCl 4,81), prosječno vrlo slabo opskrbljena fiziološki aktivnim fosforom (P2O5 4,39) i slabo opskrbljena kalijem (K2O 6,57 mg/100 g). Unatoč relativno nepovoljnim agroklimatskim uvjetima tijekom vegetacije (mjesečni prosjek 49 mm padavina) suncokret je dao zadovoljavajući prosječni prinos zelene mase od 410 dt/1 ha, i suhe tvari 75 dt/1 ha, s prosječnom hranidbenom vrijednošću u 1 kg suhe tvari; probavljivi protein 65 gr., NEL 4,96 MJ i NEM 4,87 MJ. Kod sirka je utvrđen prosječni prinos zelene mase od 260 dt/1 ha i suhe tvari 67 dt/1 ha, s prosječnom hranidbenom vrijednošću, probavljivog proteina 41 gr., NEL 4,79 MJ i NEM 4,60 MJ/1 kg suhe tvari. Prosječni sadržaj probavljivog proteina u suhoj tvari, na svim pokusnim parcelama, značajno je veći kod suncokreta (65 gr) u odnosu na hibridni sirak (41 gr), P0,01. Iako su kod suncokreta utvrđene više vrijednosti u ostvarenom prosječnom sadržaju NEL-a i NEM-a po 1 kg suhe tvari u odnosu na hibridni sirak, te razlike nisu značajne (P>0,05; P>0,01). Prosječno, na svim parcelama, po 1 ha ostvareno je kod suncokreta 38.400 MJ NEL-a i 37.703 MJ NEM-a, dok su kod sirka ostvareni nešto manji prinosi, i to 32.285 MJ NEL-a i 31.004 MJ NEM-a. Probavljivoga proteina ostvareno je prosječno po 1 ha, kod suncokreta 486 kg, a kod hibridnoga sirka značajno manje, 276 kg. Prema utvrđenim rezultatima provedenoga istraživanja, uzgojem suncokreta kao postrnoga usjeva i ostvarene neto energije po 1 ha izražene u NEL-u i NEM-u, postoji mogućnost proizvodnje oko 10.971 kg mlijeka (cca 3,5 MJ NEL/1 kg) i 754 kg prirasta (cca 50 MJ NEM/1 kg), dok se uzgojem sirka može proizvesti oko 9.224 kg mlijeka i 620 kg prirasta.Increase of fodder production on cultivated acreages of the College of agriculture in Križevci, by introducing sunflower (Helianthus annuss) and hybrid sorghum (Sorghum sp., „Grazer“) as the second intercrop into the crop rotation has been studied. The second sowing of each intercrop was performed on four plots, each around 1000 m2. The preceding crop was common wheat and liquid manure was used as a preceding fertilizer with around 85 m3/1ha. The soil on all investigated plots was acid humus (humus 3.88%; pH in 1 M KCl 4.81), averagely very weak poor in physiologically active phosphorus (P2O5 4,39) and in potassium (K2O 6,57 mg/100g). Despite relatively unfavourable agroclimatic conditions during the vegetation (average monthly precipitation 49mm) average yield of sunflower green mass was satisfactory and amounted to 410 dt, dry matter 75 dt/1ha with average nutrient value in 1kg of dry matter; digestible protein 65 gr., NEL 4.96 MJ and NEM 4.87 MJ. Determined average yield of hybrid sorghum green mass was 260 dt and dry matter 67 dt/1ha with average nutrient value, digestible protein 41 gr, NEL 4.79 MJ and NEM 4.60 MJ/1kg of dry matter. Average digestible protein content in dry matter of all investigated plots was significantly higher in sunflower (65 gr) than in hybrid sorghum (41 gr), P0.01. Although higher values of average NEL and NEM content in 1kg of dry matter were determined in sunflower than in hybrid sorghum, the differences were not significant (P>0.05; P>0.01). Average sunflower yields of 38.400 MJ NEL and 37.703 MJ NEM per 1 ha were achieved on all plots, while determined hybrid sorghum yields were lower, 32.285 MJ NEL and 31.004 MJ NEM. Average yield of digestible protein per 1 ha was 486 kg for sunflower and hybrid sorghum significantly less than 276 kg. Based on determined results of sunflower grown as intercrop the yield of net energy per 1 ha presented in NEL and NEM, allows an average production of 10.971 kg of milk (around 3.5 MJ NEL/1kg) and 754 kg of beef (around 50 MJ NEM /1kg) and growing hybrid sorghum as an intercrop allows an average production of 9.224 kg of milk and 620 kg of beef

QUANTITATIVE INDICATORS OF MILK QUALITY AND NUTRITIONAL STATUS OF SIMMENTAL AND HOLSTEIN COWS IN THE KALNIK PIEDMONT REGION

Provedeno je istraživanje u svrhu utvrđivanja pokazatelja kakvoće mlijeka i hranidbenog statusa simentalskih i holstein krava na četiri gospodarstva potkalničkoga kraja. Dva gospodarstva drže simentalske krave (ASM, B-SM), a dva crne holstein krave (A-HF, B-HF). Kod odabranih deset krava svakoga gospodarstva istraživanja su provedena na bazi II. i III. laktacije. Kontrole su se vršile jednom mjesečno dostavom uzoraka u Središnji laboratorij za kontrolu mlijeka HSC gdje je vršena uobičajena analiza mlijeka putem koje se došlo do potrebnih pokazatelja kakvoće mlijeka i ureje. Sve krave koje su bile predmetom istraživanja držane su u približno istim ekološkim uvjetima i kod njih je primjenjivana približno ista tehnologija proizvodnje mlijeka. Ostvarene razlike u dnevnoj proizvodnji mlijeka kod simentalskih i holstein krava unutar svakoga gospodarstva, na bazi istraživane II. i III. laktacije, nisu značajne (P>0,05), dok su između svih gospodarstava, neovisno o pasmini, razlike značajne (P<0,05). Pomoću koeficijenta regresije i jednadžbi regresije na gospodarstvu AHF (y1) utvrđena je pozitivna slaba veza (r1 = 0,133), dok je na drugom gospodarstvu B-HF (y2) utvrđena također pozitivna, ali relativno jaka veza (r2= 0,654) između postignute prosječne dnevne mliječnosti i sadržaja ureje u mlijeku (mg/1dl) holstein krava na oba gospodarstva. Na gospodarstvima sa simentalskim kravama A-SM (y1) i B-SM (y2) utvrđene su pozitivne osrednje jake veze između postignute prosječne dnevne mliječnosti i sadržaja ureje u mlijeku, kod A-SM r1 = 0,491, a kod B-SM r2 = 0,334. Samo kod četiri grla simentalske pasmine na gospodarstvu A-SM, od svih 40 krava koje su bile predmetom istraživanja, registriran je sadržaj ureje u mlijeku ispod 15 mg/dl, dok se sva ostala grla nalaze u granicama tzv. normalnog sadržaja ureje u mlijeku, što ujedno znači da su obroci krava na razini ostvarene mliječnosti, relativno dobro uravnoteženi glede sadržaja energetske i proteinske komponente, uzevši u obzir utvrđen povoljan sadržaj proteina (3,19-3,38%) i mliječne masti u mlijeku (3,92-4,49%). Utvrđeni rezultati parcijalnih mliječnosti (dijelova laktacije) pokazuju kod svih krava očekivane vrijednosti standardne devijacije i to kod simentalskih krava od 365 do 804 kilograma a kod holstein pasmine od 688 do 1183 kilograma, uz uobičajenu varijabilnost koja se kretala u rasponu od 8,71 do 17,74% kod svih grla.Aimed at determining the indicators of milk quality and nutritional status of Simmental and Holstein cows investigations were conducted at four husbandries in the Kalnik piedmont region. Two husbandries own Simmental cows (A-SM, B-SM) and other two own Holstein cows (A-HF, B-HF). Investigations were conducted on the basis of 2nd and 3rd lactation on ten selected cows of each husbandry. Control checks were performed at the Central laboratory for milk control of the Croatian Livestock Centre by delivering samples once per month where usual analyses of milk were performed that provided needed indicators of milk quality and milk urea. With all studied cows similar milk production technology was used and all cows were held in similar environmental conditions. On the basis of the investigated 2nd and 3rd lactation realized differences in milk production per day were not significant (P>0.05) within each husbandry of Simmental and Holstein cows, but between all husbandries differences were significant (P<0.05), not dependent on breed. The results of correlation coefficient and regression analysis for A-HF (y1) husbandry show positive and weak correlation (r1 = 0.133) while on the husbandry B-HF (y2) results show positive but relatively strong correlation (r2 = 0.654) between achieved mean milking capacity per day and the concentration of milk urea (mg/1dl) at both husbandries of Holstein cows. Positive and moderately strong correlations were determined at simmental cow husbandries A-SM (y1) and B-SM (y2) between achieved mean milking capacity per day and the concentration of milk urea, at A-SM r1 = 0.491 and B-SM r2 = 0.334. The results of milk urea concentration below 15 mg/dl were determined at A-SM husbandry on four Simmental cows of 40 studied cows and all other cows were in the range of normal concentration of milk urea which means that ratios were on the level of realized milking capacity relatively well balanced in energy and protein component regardless of determined good concentration of protein (3.19-3.38%) and milk fat (3.92-4.49%). Investigation results of partial milking capacity (parts of lactation) of all cows show expected values of standard deviation from 365 to 804 kg with Simmental cows and from 688 to 1183 kg with Holstein cows with common variability at all heads ranging 8.71 to 17.74%

Određivanje točke ledišta u uzorcima kravljeg mlijeka konzerviranog azidiolom

The study involved determination of the freezing point of cow milk by a reference (thermistor cryoscopy) and an instrumental (infrared spectrometry) method. The aim of the study was to evaluate the possibility of milk freezing point determination in milk samples preserved with azidiol by using a reference and an instrumental method of analysis. Five hundred cow milk samples were analysed during three research periods. Samples were taken at milk collection points in north-western Croatia. Samples preserved with azidiol (0.3 mL azidiol/40 mL; 0.011 g sodium azide/40 mL) and without preservatives (control samples) were analysed. The freezing point of milk was determined in duplicate. Average freezing point results of azidiol preserved samples were lower compared to control samples. A statistically significant difference between the means of the results obtained for azidiol preserved and control samples was determined (P<0.05; P<0.01) in all research periods. The results revealed a significant influence of the preservative azidiol on milk freezing point determination regardless of the method of analysis applied, which could lead to wrong interpretation of the results.Provedeno je istraživanje određivanja točke ledišta kravljeg mlijeka referentnom (termistor krioskopskom) i instrumentalnom metodom (infracrvena spektrometrija). Cilj istraživanja bio je utvrditi mogućnost određivanja točke ledišta kravljeg mlijeka u uzorcima konzerviranim azidiolom referentnom i instrumentalnom metodom. Tijekom triju istraživanih razdoblja ukupno je analizirano 500 uzoraka kravljeg mlijeka. Uzimani su na sabiralištima sjeverozapadne Hrvatske. Ispitani su nakon konzerviranja azidiolom (0,3 mL azidiola/40 mL; 0,011 g natrij azida/40 mL) i bez konzervansa (kontrolni). Točka ledišta određivana je u duplikatu. Utvrđeno je da su prosječni rezultati analiza točke ledišta uzoraka kravljeg mlijeka konzerviranog azidiolom niži u usporedbi s kontrolnim. Utvrđena je statistički značajna razlika (P<0,05; P<0,01) između prosječnih vrijednosti analiza uzoraka konzerviranih azidiolom i kontrolnih uzoraka u svim razdobljima istraživanja. Na temelju rezultata istraživanja zaključuje se da konzervans azidiol značajno utječe na određivanje točke ledišta kravljeg mlijeka neovisno o metodi ispitivanja, što može dovesti do krivog tumačenja rezultata analiza

INFLUENCE OF FERTILIZATION ON YIELD, CHEMICAL COMPOSITION AND NUTRITIVE VALUE OF MAIZE COB AND GRAIN

Radi utvrđivanja optimalne gnojidbe i njezinog utjecaja na kemijski sastav i hranidbenu vrijednost klipa i zrna kukuruza (OSSK 444), vršena su ispitivanja tijekom tri godine (2004.-2006.) na površinama ekonomije Visokog gospodarskog učilišta u Križevcima. Pokus je postavljen po slučajnom blok rasporedu u četiri ponavljanja. Veličina parcele svakoga tretmana je bila 70 m2 (7x10 m), a tretmani su bili: 1 - bez gnojidbe; 2 - N80P100K0; 3 - N80P0K100; 4 - N0P100K100; 5 - N80P100K100; 6 -N120P100K100; 7 - N120P200K200; 8 - N160P200K200; 9 - N200P200K200; 10 -N240P200K200. Analizom tla utvrđena je blago kisela reakcija (pHKCl = 5,93), nizak sadržaj humusa (1,99%), te osrednja opskrbljenost fosforom i kalijem (135,00 i 157,00 mg/kg). Analiza klipa i zrna kukuruza obavljena je metodom po Weendeu, a na osnovi utvrđenih sirovih organskih hranjivih tvari i pepela u suhoj tvari, izračunata je metabolička energija (ME) i neto energija za laktaciju (NEL). Rezultati istraživanja su pokazali da postoji značajan utjecaj gnojidbe na sadržaj sirovoga proteina u klipu (91,99 - 108,35 g/kg) i zrnu (109,48 - 128,72 g/kg), dok je utjecaj gnojidbe na sadržaj sirove vlaknine utvrđen samo u klipu kod pojedinih gnojidbenih tretmana (P0,05).In order to establish the most appropriate treatment, an analysis of the influence of various fertilization treatments on chemical compound of maize cob and grain (OSSK 444) was made on the college farm in Križevci in a three-year period (2004-2006). The experimental trial was organized in four repetitions and in a randomized block scheme. The size of the plot for each treatment was 70 square meters (7x10 m), and the treatments were as follows: 1 – no fertilization; 2 - N80P100K0; 3 - N80P0K100; 4 - N0P100K100; 5 - N80P100K100; 6 -N120P100K100; 7 - N120P200K200; 8 - N160P200K200; 9 - N200P200K200; 10 -N240P200K200. The soil analysis established a mild acid reaction (pH in KCL = 5.93), low humus content (1.99%) and medium supply level of phosphorus and potassium (135 and 157 mg/kg). The analyses of the maize grain and cob were made according to Weende. The metabolic energy (ME) and net energy for lactation (NEL) were calculated on the basis of the determined crude organic nutrients and crude ash in maize grain and cob dry matter. The results of this research lead to the conclusion that the fertilization influences the crude protein content in maize cob (91.99 – 108.35 g/kg) and grain (109.48 and 128.72 g/kg), while only certain fertilization treatments influence the crude fibre content in maize cob (P<0,05). However, fertilization does not significantly influence the average crude oil content, crude NET and the nutritive value of maize cob and grain (ME and NEL)

Određivanje točke ledišta u uzorcima kravljeg mlijeka konzerviranog azidiolom

The study involved determination of the freezing point of cow milk by a reference (thermistor cryoscopy) and an instrumental (infrared spectrometry) method. The aim of the study was to evaluate the possibility of milk freezing point determination in milk samples preserved with azidiol by using a reference and an instrumental method of analysis. Five hundred cow milk samples were analysed during three research periods. Samples were taken at milk collection points in north-western Croatia. Samples preserved with azidiol (0.3 mL azidiol/40 mL; 0.011 g sodium azide/40 mL) and without preservatives (control samples) were analysed. The freezing point of milk was determined in duplicate. Average freezing point results of azidiol preserved samples were lower compared to control samples. A statistically significant difference between the means of the results obtained for azidiol preserved and control samples was determined (P<0.05; P<0.01) in all research periods. The results revealed a significant influence of the preservative azidiol on milk freezing point determination regardless of the method of analysis applied, which could lead to wrong interpretation of the results.Provedeno je istraživanje određivanja točke ledišta kravljeg mlijeka referentnom (termistor krioskopskom) i instrumentalnom metodom (infracrvena spektrometrija). Cilj istraživanja bio je utvrditi mogućnost određivanja točke ledišta kravljeg mlijeka u uzorcima konzerviranim azidiolom referentnom i instrumentalnom metodom. Tijekom triju istraživanih razdoblja ukupno je analizirano 500 uzoraka kravljeg mlijeka. Uzimani su na sabiralištima sjeverozapadne Hrvatske. Ispitani su nakon konzerviranja azidiolom (0,3 mL azidiola/40 mL; 0,011 g natrij azida/40 mL) i bez konzervansa (kontrolni). Točka ledišta određivana je u duplikatu. Utvrđeno je da su prosječni rezultati analiza točke ledišta uzoraka kravljeg mlijeka konzerviranog azidiolom niži u usporedbi s kontrolnim. Utvrđena je statistički značajna razlika (P<0,05; P<0,01) između prosječnih vrijednosti analiza uzoraka konzerviranih azidiolom i kontrolnih uzoraka u svim razdobljima istraživanja. Na temelju rezultata istraživanja zaključuje se da konzervans azidiol značajno utječe na određivanje točke ledišta kravljeg mlijeka neovisno o metodi ispitivanja, što može dovesti do krivog tumačenja rezultata analiza