SpermMotility

Data on sperm motility (% motile sperm ) for all male

ReadMe Zajitschek etal BMCEvolBiol 2017

Information in data filters applie

OffspringActivity

Raw data on offspring activity from video recording

MalePCs

Data from PCAs to combine behavioural parameter

DominanceStatus

Dominance status for each male over both experimental rounds

Additional file 1: Supplemental Material. of Paternal personality and social status influence offspring activity in zebrafish

Methods: 1. Personality assays. 2. Sperm measurements. 3. In vitro fertilisation. Figure S1: The number of minutes the fish were tracked for was not related to the activity measures. (A) There was no relationship between number of frames fish were tracked and their mean median speed (Spearman Correlation: r = −0.05, p = 0.59). (B) There was also no relationship between the mean median speed of fish in a trial, and the time when the tracking begun (Spearman Correlation: r = 0.08, p = 0.41). Table S1: The loadings of the first three principal components for each of the different behavioural trials and their explanatory power. PC1pre for all trials can be interpreted along the bold-shy continuum (indicated by loadings in bold). *“Time to start” refers to the time an individual took to cross a threshold line for the first time (Dive, Novel Object, Open Field) or exit the shelter for the first time (Shelter). “# in centre” / “time in centre” represent the number of times / amount of time a focal male spent within the area of a specified threshold. “# freezing bouts” and Time immobile refer to anxious behaviour, where sudden erratic movements and remaining entirely still may alternate. Table S2: Sperm longevity (in seconds since activation), ejaculate volume (μl) and motility (% of motile sperm cells), after the first round of dominance trials and after the second (taking the social status of the first round into account, MSS). Interaction terms were not significant and were hence not included. Degrees of Freedom are based on Satterthwaite approximations for type III ANOVA. Table S3: Effects of male behaviour and male social status on sperm velocity measures VCL (curvilinear velocity); VSL (straight-line velocity); VAP (average path velocity) after round 2. “Time” indicates the decline in velocity measured in 10 s steps since activation, “Time2” investigates the curvature in the decline. Significant explanatory variables are highlighted by an asterisk (*). (DOCX 101 kb