HPLC analysis of harringtonine and homoharringtonine in the needles of <i>Cephalotaxus griffithii</i> alkaloid fraction and cytotoxic activity on chronic myelogenous leukaemia K562 cell

<div><p>Harringtonine (HT) and homoharringtonine (HHT) are <i>Cephalotaxus</i> alkaloids with considerable antileukaemic activity. The objectives of this research were to (1) determine the content of HT and HHT present in <i>Cephalotaxus griffithii</i> needles alkaloid fraction (CGAF) and (2) compare the antiproliferative activity of CGAF, with that of HT and HHT on chronic myelogenous leukaemia K562 cell. The concentration of HT and HHT was found to be 122.14 and 16.79 mg/g of CGAF, respectively. Treatment of K562 cells with CGAF, HT and HHT decreased the viable cells in a dose- and time-dependent manner. Interestingly, the maximum cell death was found in CGAF, with IC₅₀ value which was 3- to 4.6-fold lower than those of HT and HHT. Our results indicate that HT content in the needles of <i>C. griffithii</i> is higher than HHT, and alkaloids other than HT and HHT in CGAF are predominantly responsible for K562 cell death.</p></div

Essential oil of <i>Cephalotaxus griffithii</i> needle inhibits proliferation and migration of human cervical cancer cells: involvement of mitochondria-initiated and death receptor-mediated apoptosis pathways

<div><p>This study was conducted to determine the effect of <i>Cephalotaxus griffithii</i> needle essential oil (CGNO) on proliferation and migration of human cervical cancer (HCC) cells. CGNO treatment decreased the viability of all the tested HCC (HeLa, ME-180 and SiHa) cells. Morphological and DNA fragmentation analysis of CGNO-treated HeLa cells indicated the involvement of apoptosis in inducing HCC cell death. CGNO increased mitochondrial membrane depolarisation and upregulated the expression of caspase-9, caspase-8, caspase-3 and cleaved-PARP. The activity of caspase-8 and caspase-9 was also significantly increased. Wound healing and transwell migration assay demonstrated that CGNO significantly inhibited the migration of HeLa cells to close a scratched wound and also inhibited their migration through filter towards a chemotactic stimulus. Taken together, these results indicated that CGNO inhibited the proliferation and migration of HCC cells. Of note, CGNO induced HeLa cell death through mitochondria-initiated and death receptor-mediated apoptosis pathway.</p></div

Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3

The scientific data summarizes identified peptide spots from two dimensional gel electrophoresis. Identified spots are secretory products from the cellulolytic activity of Talaromyces verruculosus SGMNPf3. Peptide spots were identified by LC MS/MS and MALDI TOF-MS and detail characteristics of spots are provided

Figure 1-Phylogenetic and morpgological placement of Aspergillus terreus

Figure 1 Molecular phylogenetic analysis by Maximum likelihood method (ML) based on the K2 + G substitution model. AIC is 1953.78, BIC is 2311.02; the highest log likelihood is -953.45 and bootstrap values ≥ 50% from 1000 iterations are shown. Blue highlighted strain of A. terreus (GenBank® accession number KC305600) causes foliar necrosis of potato. The ML analysis was performed in MEGA 6 [34]. Morphological characteristics of globular accessory conidia indicated arrows stained with Rose Bengal, broom-like conidiophore and colony on PDA, scale bar = 20 µm and magnification = 1000X

File 1-Muscle-aligned cadmodulin genes of Aspergillus terreus

Multiple sequence alignment of partial sequences of Cadmodulin gene of Aspergillus terreus. The sequences were aligned using Muscle software in MEGA 6.01 using default settings