Propionibacterium acnes CAMP Factor and Host Acid Sphingomyelinase Contribute to Bacterial Virulence: Potential Targets for Inflammatory Acne Treatment

) permits the bacteria to spread and become in contact with various skin and immune cells.-induced inflammation. CAMP factor may hijack host ASMase to amplify bacterial virulence to degrade and invade host cells. This work has identified both CAMP factor and ASMase as potential molecular targets for the development of drugs and vaccines against acne vulgaris

Application of industrial treatments to donor human milk: influence of pasteurization treatments, storage temperature, and time on human milk gangliosides

Donor milk treatment: key nutrients preserved after pasteurization Donor human milk, the best alternative to mother’s own milk, usually needs to be pasteurized before use out of safety concerns. Daniela Barile at University of California Davis, USA, and colleagues studied the effects of heat treatment and storage temperature and time on milk gangliosides, a class of sugar-derived compounds important for neural and brain development of newborns, among other bioactivities. They found that, while there were minor structural changes during mimicked industrial pasteurization processes, gangliosides remain stable for at least three months either in the refrigerator or at room temperature. These results may help standardize the processing protocols and storage conditions for donor milk, and the methods can be extended to other bioactive components

Mice lacking ganglioside GM3 synthase exhibit complete hearing loss due to selective degeneration of the organ of Corti

The ganglioside GM3 synthase (SAT-I), encoded by a single-copy gene, is a primary glycosyltransferase for the synthesis of complex gangliosides. In SAT-I null mice, hearing ability, assessed by brainstem auditory-evoked potentials (BAEP), was impaired at the onset of hearing and had been completely lost by 17 days after birth (P17), showing a deformity in hair cells in the organ of Corti. By 2 months of age, the organ of Corti had selectively and completely disappeared without effect on balance or motor function or in the histology of vestibule. Interestingly, spatiotemporal changes in localization of individual gangliosides, including GM3 and GT1b, were observed during the postnatal development and maturation of the normal inner ear. GM3 expressed in almost all regions of cochlea at P3, but at the onset of hearing it distinctly localized in stria vascularis, spiral ganglion, and the organ of Corti. In addition, SAT-I null mice maintain the function of stria vascularis, because normal potassium concentration and endocochlear potential of endolymph were observed even when they lost the BAEP completely. Thus, the defect of hearing ability of SAT-I null mice could be attributed to the functional disorganization of the organ of Corti, and the expression of gangliosides, especially GM3, during the early part of the functional maturation of the cochlea could be essential for the acquisition and maintenance of hearing function

CMIP is a negative regulator of T cell signaling

International audienceUpon interaction with cognate antigen, T cells integrate different extra and intracellular signals, involving basal and induced protein-protein interactions as well as binding of proteins to lipids, which can lead to either cell activation or inhibition. Here we show that selective T-cell expression of CMIP, a new adaptor protein, by targeted transgenesis drive