UJI HALAL BOVINE DEMINERALIZED BONE MATRIX

Background Demineralized Bone Matrix (DBM) is a biomaterial product derived from long bones of bovine, functioning as a graft in the process of new bone formation. DBM contains 93% collagen type I, 5% protein growth factor and 2% remaining osteocyte cells. Collagen acts as an extra cellular matrix that binds to osteoblasts. One of the proteins contained in DBM is Bone Morphogenetic Protein (BMP). This protein triggers DBM mineralization to form new bone. the majority of issues related to halal are related to the existence of swine-based biomaterials. Purpose Halal analysis methods must be developed to ensure the authentication of raw materials for products, finished products and product counterfeiting. Analysis and authentication of products is urgently needed along with the emergence of health problems and sensitivity among consumers. Requirements for animal-based products to be halal, animal types must be considered and proven that there is no contamination with illicit substances during the production process of DBM is widely used in bone defect therapy in humans, because it comes from mammals, it is necessary for halal tests to ensure that DBM does not originate from swines. Method The DBM test uses a biomolecular examination that is designed with two primers for the analysis of mitochondrial DNA of osteocyte cells. DNA isolation in DBM were amplified using PCR with specific primers of Cytochrome B gene markers in bovine mitochondria of 315 basepairs (bp) and Cytochrome B gene markers of swine mitochondria of 216 bp. Result DBM graft showed positive results on bovine cytochrome B genes and negative results on swine cytochrome B genes. Conclusion DBM graft produced by Dr. Soetomo Hospital Tissue Bank is a halal biomaterial and not contaminated by swine

Environmental Prevention Programs on Combating the COVID-19 Pandemic by Community Participation

COVID-19 is a new disease that has become a pandemic. This disease must be wary of because of relatively rapid transmission, has a mortality rate that cannot be ignored, and the absence of definitive therapy. In Indonesia, 463,007 cases with COVID-19 and 15,148 deaths have been established on November 2020. To determine the distribution that goes fast, and education on prevention and disinfection have not been adequately spread in the community. Candisari, was a village located in the middle of teak forest with several entrances to the residents' villages. This condition is the main cause of the high risk of disease transmission from people entering and leaving the village, besides prevention measures have not been optimal. The development of educational methods, assistance for the prevention and disinfection of the environment of the village of Candisari has been compiled by the community service team of Airlangga University. Research on anti-virus disinfecting materials has also been developed at Airlangga University. The synergy between academia and the community is expected to be able to improve the understanding of citizens and prevent transmission of COVID 19 in Candisari village, Sambeng sub-district, Lamongan regency

Examining Caries Risk With the Caracteristic of HLA-DRB1 Gene

Introduction: HLA-DRB1 alleles were derived from MHC class II molecules. These alleles encoded sIgA secretion which contribute as a barrier to S. mutans colonization. HLA-DRB1 was known as genes with high mutations causing differences in peptide bond, thus affecting the progression and vulnerability to a disease. The purpose of this study was to determine the effect of mutations in HLA-DRB1 alleles in different dental caries risk. Methods: In this study, we extracted the genomic DNA from whole blood samples of 30 patients with low level dental caries (indeks def-t 3) as case group. HLA-DRB1 varians were studied through genomic DNA isolation for PCR-RFLP. RFLP is analyzed through BseRI, BsaJI, RsaI, and Sau961 restriction enzymes was used in this assay. Results: The PCR-RFLP typing method was evaluated on 60 genomic DNA samples, result found that all samples were divided into 5 groups of variants, two variants in the control group and three variants in the case group. Conclusion: PCR-RFLP was shown to be a sensitive method for the detection of mutation in HLA-DRB1 alleles caused a dental caries level differences. HLA-DRB mutations caused changes in signal transduction and therefore contributes to imunogenetik pathway of dental caries

The Role of Demineralized Dentin Material Membrane as Guided Bone Regeneration

Guided Bone Regeneration (GBR) is a method treating a biomaterial that is located into the injured bone to prevent fibrous tissue from entering the injured bone area before the replacement bone grown completely, divided into polytetrafluoroethylene (e-PTFE), synthetic biodegradable polyester, and collagen membranes. The aim of this study is to present an evaluation of literature regarding the rule of novel Demineralized Dentin Material Membrane (DDMM) as GBR. Bone defect after surgical or trauma procedures can be corrected through several mechanisms, which depend on the level of restriction, the level of bone injury, and the physiological process. The use of guided bone regeneration aims to ensure perfect new bone growth, with the exact same shape as the original bone. The application procedure of DDMM as a GBR therapy for bone injury on craniomaxillofacial could be provide bone cells for bone regeneration and protects the bone regeneration process from non-osteogenic tissues

The role of salivary sIgA as protection for dental caries activity in Indonesian children

Purpose: The aim of this study is to asses the relationship between the level of sIgA and dental caries experience in healthy children who are 6- to 9-years-old from Indonesia. The case-control study is conducted to determine the protective role of salivary secretory immunoglobulin A (sIgA) levels in the stimulated whole saliva of dental caries-active and caries-free children. Methods: This research was done by stimulating the whole saliva which had been collected from 6- to 9-years-old children with the index def-t≥3 of 30 children as the caries-active children group and 30 children with def-t<3 as the low caries-active children group. Saliva samples were collected in sterile vials between 10 am-12 pm due to the circadian rhythm, which is at least one hour after last meal. 1,5 ml of collected salivary sample was centrifuged, then the supernatants was transferred to other tube and stored immediately to the laboratory at a temperature of –20 °C. The estimation of sIgA concentration was done by using ELISA. The differences in the level of sIgA between the two groups with caries were analyzed using the t-test afterward. Results: The total salivary concentration of sIgA was statistically significantly higher in the low caries-active children group than in the caries-active children group. Conclusion: The total salivary concentration of sIgA was statistically and significantly higher in the low caries-active children group than caries-active children Group. There is a negative correlation between sIgA level and dental caries activity of 6 to 9-years-old children

Guided Bone Regeneration to Improve Osseointegration in Dental Implant

Prosthetic oral rehabilitation using dental implant has significantly increase because of the patient’s esthetic necessity and as predictable treatment. There is 40-60% of alveolar bone width and height loss in 2-3 years post-extraction. Dental implant mechanical stability depends on the successful of osseointegration and healthy soft tissue attachment over the abutment as a result of healing post-implantation. Inadequate bone volume at the time of implantation have been associated with decreased success rates and long term prognosis in implant dentistry. Guided bone regeneration (GBR) can be used with or without combination of bone graft to gain the volume of bone for implant placement. The benefit of using membrane GBR in dental implants is to accelarate implant placement by enhance bone formation surrounding implant material and as a result osseointegration can be achieve

In vitro cell proliferation assay of demineralized dentin material membrane in osteoblastic MC3T3-E1 cells Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

Aim: Demineralized dentin material membrane (DDMM) is a novel bioresorbable guided bone regeneration (GBR) which is derived from the demineralization process of bovine dentin. This material/process could be an alternative to resolve musculoskeletal dysfunction that harms the quality of human life. Purpose: To evaluate the cytotoxic effect of DDMM as GBR membrane on MC3T3-E1 osteoblast cell line. Methods: Cytotoxic effect was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Osteoblast MC3T3-E1 cell culture was used as a parameter of cell viability after reacting with GBR materials. The absorbance values were examined at each treatment to determine the percentage of cell viability. There were four groups created in the present study: two treatment groups and two control groups. The treatment groups consisted of a DDMM group and a bovine pericardium collagen membrane (BPCM) group. The control groups comprised a group containing cell culture medium as a negative control group and another positive control group that contained cell cultures. Results: The results revealed no significant difference in MC3T3-E1 cell viability between the treatment and control groups (p < 0.05). Moreover, as observed in the DDMM group, there was an increase in the number of osteoblast cells. Conclusion: DDMM is a suitable alternative biomaterial for GBR as it is non-cytotoxic and could potentially increase the rate of repair of craniofacial defects

Oral Health Knowledge, Attitude and Behaviour of Indonesian Dental Students in East Java Province, Indonesia

Background: Oral health knowledge, attitudes and behaviors possessed by dental students become provisions in the education and promotion of oral health in the community. Purpose: This study aims to analyze the oral health knowledge, attitudes and behavior of Indonesian dental students in East Java province based on gender and educational stage. Methods: This research is a cross-sectional study using an online questionnaire distributed to dental students at five dental faculties in East Java. 169 respondents in this study completed an online HU-DBI questionnaire with a choice of answers to agree or disagree about the description of oral health knowledge, attitudes, and behavior. Results: Female students have a higher level of knowledge and oral health behavior than the opposite sex with a significance value of <0.001 and 0.05, respectively. There was a significant correlation between knowledge and attitude with a significance level of 0.030 and a correlation between knowledge and behavior with a significant number of 0.037. Conclusion: Female dental students had better oral health knowledge and behavior than male dental students. There is no relationship between the education stage and oral health knowledge, attitudes and behaviour. Further, we found positif association between oral health knowledge and attitudes towards behaviour