Effect of combination of vitamin B1 and B6 on leg cramps in pregnancy

چکیده: زمینه و هدف: گرفتگی عضله انقباض دردناک غیر ارادی و موضعی یک عضله یا قسمتی از یک عضله است. بین 5 تا بیش از 30 زنان از گرفتگی عضلات پا حین حاملگی شکایت دارند. منیزیم رل مهمی در متابولیسم و فعالیت عضلانی بر عهده دارد، بدلیل نبودن ترکیب مناسبی از منیزیم در ایران و ارتباط و تداخل عملکرد منیزیم و ویتامین های B1 و B6 و همینطور بی خطر بودن مصرف ویتامین ها در حین حاملگی، این مطالعه با هدف بررسی اثر درمانی ترکیب ویتامین B1 و B6 روی گرفتگی عضلات پا حین حاملگی انجام گرفت. روش بررسی: در این کار آزمایی بالینی آینده نگر دو سوکور 63 زن مبتلا به گرفتگی عضلات پا از نظر تعداد، شدت درد و زمان وقوع گرفتگی مورد بررسی قرار گرفتند. سپس به آنها به صورت تصادفی یک قوطی محتوی 56 کپسول حاوی 250 میلی گرم ویتامینB1 و 240 میلی گرم ویتامینB6 و یا 56 کپسول مشابه محتوی نشاسته داده شد و به ایشان گفته شد برای چهار هفته روزانه دو کپسول میل کنند. سپس بیماران مجدداً از نظر تعداد، شدت درد و زمان وقوع گرفتگی و ارزیابی آنها از اثر درمان بررسی شدند. یافته ها: پس از اتمام درمان تعداد و شدت درد گرفتگی عضلات پا در گروه دارو به طور معنی داری از گروه دارو نما کمتر بود (05/0>p). ولی تفاوت معنی دار بین دو گروه در زمان وقوع گرفتگی ها دیده نشد. در این مطالعه هیچ عارضه جانبی با مصرف دارو یا دارو نما دیده نشد. نتیجه گیری: به نظر می رسد که تجویز ترکیب ویتامین های B‏1 و B6 در کاهش تعداد و شدت درد گرفتگی عضلات پا حین حاملگی مؤثر باشد

Pojedyncza torbiel bąblowcowa w mięśniach przykręgosłupowych w odcinku szyjnym: opis przypadku i przegląd piśmiennictwa

Hydatid disease caused by Echinococcus granulosus and Echinococcus multilocularis commonly presents with pulmonary and hepatic cysts. Primary paraspinal muscle cysts are a rare presentation. We report a case of hydatid cyst within paraspinal muscles presenting with cervical mass and associated pain. The hydatid disease serological test was negative. Neither hepatic nor pulmonary cystic lesions were found. Radiographic findings were unspecific for hydatid cysts. Surgical resection was planned due to the provisional diagnosis of muscular cystic neoplasm. During surgery, a cyst containing a clear liquid was found. The cyst wall was excised and the surgical field was irrigated with hypertonic saline. The patient's symptoms resolved by discharge day. Postoperative pathological examinations revealed a muscular hydatid cyst.Bąblowica spowodowana przez Echinococcus granulosus lub Echinococcus multilocularis często przejawia się torbielami płuc lub wątroby. Pierwotne torbiele w mięśniach przykręgosłupowych są rzadką manifestacją choroby. W pracy opisano przypadek torbieli bąblowcowej w mięśniach przykręgosłupowych, która objawiała się guzem szyi i bólem. Badania serologiczne w kierunku bąblowicy dały wynik ujemny. Nie stwierdzono również zmian w wątrobie ani w płucach. Wyniki badań obrazowych nie były swoiste dla torbieli bąblowcowej. W związku z roboczym rozpoznaniem torbielowatego nowotworu mięśnia przeprowadzono operację, w trakcie której stwierdzono torbiel wypełnioną przezroczystym płynem. Torbiel wycięto wraz z jej ścianą, a pole operacyjne przepłukano hipertonicznym roztworem chlorku sodu. Dolegliwości zgłaszane przez chorego ustąpiły do dnia wypisu ze szpitala. W badaniu histopatologicznym stwierdzono torbiel bąblowcową w mięśniu

Combination effect of cytochrome P450 1A1 gene polymorphisms on uterine leiomyoma: A case-control study

Uterine leiomyoma (UL) is an estrogen-dependent neoplasm of the uterus, and estrogen metabolizing enzymes affect its progression. This study aimed to evaluate the association between two single-nucleotide polymorphisms of cytochrome P450 1A1 (CYP1A1) gene and UL risk. The study consisted of 105 patients with UL and 112 healthy women as controls. Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene were analyzed by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods, respectively. The findings indicated no association between Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene and UL (p < 0.05). However, the combination effect of TT/AG genotypes of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms was associated with 4.3-fold higher risk of UL. In addition, haplotype analysis revealed that TG haplotype of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms could increase the UL risk nearly 4.9-fold. Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms of CYP1A1 gene were not associated with UL susceptibility; however, the combination of the TT/AG genotypes and TG haplotype could increase the UL risk

The −2549 insertion/deletion polymorphism of VEGF gene associated with uterine leiomyoma susceptibility in women from Southeastern Iran

Objectives: Vascular endothelial growth factor (VEGF) is an important angiogenic factor that regulates angiogenesis and mediates sex steroid-induced cell growth. The present study investigated the association of VEGF gene-2578C/A (rs699947) and −2549 insertion/deletion polymorphisms in the promoter region of VEGF-A gene and uterine leiomyoma susceptibility in Southeast of Iran. Material and methods: One hundred and fifty five women with uterine leiomyoma and 157 age, BMI, and ethnicity matched healthy women were enrolled in this study. VEGF gene –2578C/A polymorphism genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the –2549 insertion/dele­tion polymorphism was analyzed by PCR method. Results: The frequency of alleles and genotypes of VEGF-2578C/A polymorphism was not different between women with uterine leiomyoma and the controls; however, a significant association was revealed between II genotype of –2549 insertion/deletion (I/D) polymorphism of VEGF gene and uterine leiomyoma. Conclusions: The findings showed that VEGF gene –2549 insertion/deletion polymorphism was associated with uterine leiomyoma

Platelet to lymphocyte and neutrophil to lymphocyte ratio in the first trimester of pregnancy, are they useful for predicting spontaneous miscarriage? A case-control study

Background: In 15% of all clinical pregnancies, a miscarriage can occur, but the exact cause of this phenomenon is not fully understood. However, it is believed that a faulty placenta, which triggers an inflammatory response in the mother’s body, may be one of the causes. Medical literature has increasingly focused on 2 indicators of inflammation, the plateletlymphocyte ratio (PLR) and neutrophil-lymphocyte ratio (NLR). Despite this, there has yet to be a study conducted that examines the rates of PLR and NLR in cases of miscarriage. Objective: This study aims to determine whether there is an increase in complete blood count inflammatory parameters such as NLR and PLR in women who experience miscarriages. Materials and Methods: This retrospective case-control study was conducted from March 2021 to March 2022, across 3 academic hospitals in Tehran, Iran. A total of 240 participants were enrolled comprising individuals with either miscarriages or normal pregnancies (n = 120/each). Data were collected from the medical records of participants aged between 18-42 yr old, with gestational age ranging from 6-13 wk. The demographic information, including age, body mass index, parity, history of abortion, number of abortions, number of living children, hematocrit and hemoglobin levels, platelet distribution width (PDW), PLR, NLR, mean platelet volume, and platelet were extracted from their records. The gestational age was also recorded. Results: A total of 240 participants were recruited for the study. PDW, NLR, PLR, and lymphocyte values were higher in the miscarriage group compared to the healthy normal pregnant women (p &lt; 0.001). Mean platelet volumes were found to be lower in the miscarriage group compared to the healthy normal pregnant women (p &lt; 0.001). Conclusion: Although, no statistically significant difference was observed in the hemoglobin, hematocrit, platelets, and neutrophils in these 2 groups of pregnant women. The higher inflammatory markers including PDW, NLR, and PLR could potentially aid in the speculation of defective placentation as a contributing factor to the development of miscarriage. Measurement of these markers may be useful to predict pregnancy leading to miscarriage. Key words: Spontaneous abortion, Inflammation, Neutrophils, Lymphocytes, Blood platelet, Pregnancy

Streptozotocin induced oxidative stress, innate immune system responses and behavioral abnormalities in male mice

Recent evidence indicates the involvement of inflammatory factors and mitochondrial dysfunction in the etiology of psychiatric disorders such as anxiety and depression. To investigate the possible role of mitochondrial-induced sterile inflammation in the co-occurrence of anxiety and depression, in this study, we treated adult male mice with the intracerebroventricular (i.c.v.) infusion of a single low dose of streptozotocin (STZ, 0.2 mg/mouse). Using valid and qualified behavioral tests for the assessment of depressive and anxiety-like behaviors, we showed that STZ-treated mice exhibited behaviors relevant to anxiety and depression 24 h following STZ treatment. We observed that the co-occurrence of anxiety and depressive-like behaviors in animals were associated with abnormal mitochondrial function, nitric oxide overproduction and, the increased activity of cytosolic phospholipase A2 (cPLA2) in the hippocampus. Further, STZ-treated mice had a significant upregulation of genes associated with the innate immune system such as toll-like receptors 2 and 4. Pathological evaluations showed no sign of neurodegeneration in the hippocampus of STZ-treated mice. Results of this study revealed that behavioral abnormalities provoked by STZ, as a cytotoxic agent that targets mitochondria and energy metabolism, are associated with abnormal mitochondrial activity and, consequently the initiation of innate-inflammatory responses in the hippocampus. Our findings highlight the role of mitochondria and innate immunity in the formation of sterile inflammation and behaviors relevant to anxiety and depression. Also, we have shown that STZ injection (i.c.v.) might be an animal model for depression and anxiety disorders based on sterile inflammation

Diagnostic value of CD-10 marker in differentiating of papillary thyroid carcinoma from benign thyroid lesions

Background: Using of CD10 in accordance with clinical and histological features of thyroid lesions could be used as both diagnostic and prognostic tool, which consequently influence the management and their prognosis for survival of patients with thyroid neoplasms especially papillary thyroid carcinoma (PTC). The aim of this study was to determine its expression in PTC and different benign thyroid lesions. Materials and Methods: In this descriptive-analytic, cross-sectional study, paraffin-embedded tissues of patients with definitive pathologic diagnosis of different benign thyroid lesions and PTC were retrieved. Immunostained sections of each slides was performed using immunohistochemistry methods and expression of CD10 was compared in two groups of benign thyroid lesions and PTC. Results: From selected cases 134 sections studied in two groups of PTC (n = 67) and benign thyroid lesions (n = 67). CD10 were immunohistochemically positive in 29.9% of PTC cases, but in none of the thyroid benign lesions (0%) (P < 0.001). There was not significant relationship between expression of CD10 with age and sex of the studied population (P > 0.05). Conclusion: The results of the current study indicate that due to the higher expression of CD10 in PTC than benign thyroid lesions it might be used for differentiating mentioned lesions. But for using it as a diagnostic tool further studies with larger sample size and determination of its sensitivity, specificity and cut-off point is necessary

Diagnostic value of CD-10 marker in differentiating of papillary thyroid carcinoma from benign thyroid lesions

Background: Using of CD10 in accordance with clinical and histological features of thyroid lesions could be used as both diagnostic and prognostic tool, which consequently influence the management and their prognosis for survival of patients with thyroid neoplasms especially papillary thyroid carcinoma (PTC). The aim of this study was to determine its expression in PTC and different benign thyroid lesions. Materials and Methods: In this descriptive-analytic, cross-sectional study, paraffin-embedded tissues of patients with definitive pathologic diagnosis of different benign thyroid lesions and PTC were retrieved. Immunostained sections of each slides was performed using immunohistochemistry methods and expression of CD10 was compared in two groups of benign thyroid lesions and PTC. Results: From selected cases 134 sections studied in two groups of PTC (n = 67) and benign thyroid lesions (n = 67). CD10 were immunohistochemically positive in 29.9% of PTC cases, but in none of the thyroid benign lesions (0%) (P < 0.001). There was not significant relationship between expression of CD10 with age and sex of the studied population (P > 0.05). Conclusion: The results of the current study indicate that due to the higher expression of CD10 in PTC than benign thyroid lesions it might be used for differentiating mentioned lesions. But for using it as a diagnostic tool further studies with larger sample size and determination of its sensitivity, specificity and cut-off point is necessary