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Ecological relationships between marine microorganisms and hydrocarbons in the OEI study area, Louisiana
Supported by Gulf Universities Research Consortium, Project #OE73HJMCataloged from imperfect copy: all after leaf 20 wantingSeven cruise projects were conducted in association with the Louisiana Offshore Ecological Investigation (OEI) to determine the relationships between microorganisms and hydrocarbons in surface waters. Techniques were developed to take surface samples and to determine bacterial numbers, hydrocarbon content, and the response of indigenous microorganisms to various added hydrocarbon molecules. The numbers of heterotrophic bacteria as determined by dilution techniques varied from 10 to 10,000 per ml. The hydrocarbon oxidizing organisms varied from 10 to 100,000 per liter with maxima in the month of January. Hydrocarbon concentrations varied from 0.03 to 0.6 micrograms per liter. There was no correlation between the numbers of oil degraders and hydrocarbon concentration found. This may be the result of the in situ low concentrations of hydrocarbons which would limit microbial response. The numbers of bacteria and hydrocarbons were similar to control areas in the eastern and western parts of the Gulf. BOD experiments conducted to measure the response of the microorganisms to various low and high molecular weight paraffinic and aromatic hydrocarbons indicated that all hydrocarbons were oxidized by some of the mixed indigenous microorganisms. These data suggest that an adequate inoculum was naturally present to respond to oil contamination in nature. The high numbers of hydrocarbon oxidizing microorganisms associated with January hydrographic data may indicate that the upland leaching and other contamination of hydrocarbons in the Mississippi River produce the noted microbial response for that month. The dominant saturated hydrocarbons and isoprenoids ranged from C-15 to C-41. Water from Timbalier Bay generally contained higher concentrations than offshore samples and exhibited a more pronounced odd-even preference in the range C-24 to C-33. GC-MS analysis of the dominant paraffins revealed a characteristic biomodal distribution dominated by C-17 and pristane and C-25 to C-35 with an unresolved envelope. No buildup of specific n-paraffin molecules was found, which data corresponded to BOD results of added hydrocarbons to indigenous microflora.Marine Scienc
THE EFFECT OF STOCKING PROCEDURE ON CONSUMPTION OF SHELF LIFE IN REFRIGERATED SEAFOODS DISPLAYED IN FULL SERVICE DEPARTMENTS
Food Consumption/Nutrition/Food Safety,