New neolignan glycoside from the root of <i>Aralia echinocaulis</i> Hand. -Mazz

<p>A phytochemical investigation was carried out to the root of <i>Aralia echinocauis</i>, and a new neolignan glycoside, 3-{3,5-dimethoxy-4β-d-Glucopyranoside-2-[3-(3-β-d-Glucopyranoside-4-methoxy-phenyl)-allyl]-phenyl}-prop-2-en-1-ol (<b>1</b>), together with a known saponin, araliasaponin II (<b>2</b>) for the first time was obtained<i>.</i> The chemical structure of compound <b>1</b> was identified mainly by the analysis of NMR including 1D and 2D NMR in combination with High Resolution Electrospray Ionisation Mass (HR-ESI-MS). This paper herein describes the isolation and structural elucidation of compound <b>1</b>.</p

DataSheet_1_Genome-wide identification and characterization of the NPF genes provide new insight into low nitrogen tolerance in Setaria.zip

IntroductionNitrogen (N) is essential for plant growth and yield production and can be taken up from soil in the form of nitrate or peptides. The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) genes play important roles in the uptake and transportation of these two forms of N.MethodsBioinformatic analysis was used to identify and characterize the NPF genes in Setaria. RNA-seq was employed to analyze time-series low nitrate stress response of the SiNPF genes. Yeast and Arabidopsis mutant complementation were used to test the nitrate transport ability of SiNRT1.1B1 and SiNRT1.1B2.ResultsWe identified 92 and 88 putative NPF genes from foxtail millet (Setaria italica L.) and its wild ancestor green foxtail (Setaria viridis L.), respectively. These NPF genes were divided into eight groups according to their sequence characteristics and phylogenetic relationship, with similar intron-exon structure and motifs in the same subfamily. Twenty-six tandem duplication and 13 segmental duplication events promoted the expansion of SiNPF gene family. Interestingly, we found that the tandem duplication of the SiNRT1.1B gene might contribute to low nitrogen tolerance of foxtail millet. The gene expression atlas showed that the SiNPFs were divided into two major clusters, which were mainly expressed in root and the above ground tissues, respectively. Time series transcriptomic analysis further revealed the response of these SiNPF genes to short- and long- time low nitrate stress. To provide natural variation of gene information, we carried out a haplotype analysis of these SiNPFs and identified 2,924 SNPs and 400 InDels based on the re-sequence data of 398 foxtail millet accessions. We also predicted the three-dimensional structure of the 92 SiNPFs and found that the conserved proline 492 residues were not in the substrate binding pocket. The interactions of SiNPF proteins with NO3− were analyzed using molecular docking and the pockets were then identified. We found that the SiNPFs- NO3− binding energy ranged from -3.8 to -2.7 kcal/mol.DiscussionTaken together, our study provides a comprehensive understanding of the NPF gene family in Setaria and will contribute to function dissection of these genes for crop breeding aimed at improving high nitrogen use efficiency.</p

Additional file 3: of Genome-wide identification, characterization, and evolutionary analysis of flowering genes in radish (Raphanus sativus L.)

Flowering genes identified in Raphanus sativus, Brassica oleracea, and Brassica rapa. (XLS 64 kb

Histoire complète et seule véritable du petit bossu Mayeux, chasseur de la garde nationale, écrite par lui-même et suivie de sa chanson

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