<p>A phytochemical investigation was carried out to the root of <i>Aralia echinocauis</i>, and a new neolignan glycoside, 3-{3,5-dimethoxy-4β-d-Glucopyranoside-2-[3-(3-β-d-Glucopyranoside-4-methoxy-phenyl)-allyl]-phenyl}-prop-2-en-1-ol (<b>1</b>), together with a known saponin, araliasaponin II (<b>2</b>) for the first time was obtained<i>.</i> The chemical structure of compound <b>1</b> was identified mainly by the analysis of NMR including 1D and 2D NMR in combination with High Resolution Electrospray Ionisation Mass (HR-ESI-MS). This paper herein describes the isolation and structural elucidation of compound <b>1</b>.</p