Financing the U.S. Health System: Issues and Options for Change

Explores key issues of health reform and options for financing health care -- redirecting funds to more effective uses, rolling back tax cuts, modifying tax exclusions for health benefits, an employer play-or-pay model, and a value-added tax

Impact assessment of frontline demonstrations on green gram: Experience from rainfed condition of Rajasthan

Pulses being rich in quality protein, minerals and vitamins are inseparable ingredients of diet of majority of Indian population. Despite high nutritive value of pulses and their role in sustainable agriculture desired growth rate in production could not be witnessed. The domestic production of pulses is consistently below the targets and actualdomestic requirements are also higher, due to these pulses are being imported. The Krishi Vigyan Kendra Pali has carried out frontline demonstrations on green gram covering an area of 26.5 ha of farmers’ field to exhibit latest production technologies and compared it with farmer’s practice. The study in total 40 frontline demonstrations were conducted on farmers’ fields in villages viz., Kishanagar, Bedkallan, Boyal, Kushalpura and Balara of Pali district of Rajasthan state during 2014, 2015 and 2016, to demonstrate production potential and economic benefit of improved technologies comprising sowing method, nutrient management and chemical weed control and adoption of whole package of practices for the crop. After sowing of seed application of weedicide Pendimethalin (within two days after sowing) at 1.0 kg/ha in 500 liters of water used for effective control of the weeds during kharif season in rainfed condition. The findings of the study revealed that the demonstrated technology recorded a mean yield of 982 kg/ha which was 35.5% higher than obtained with farmers’ practice (755 kg/ha). Higher mean net income of Rs. 46030/ha with a Benefit: Cost ratio of 4.3 was obtained with improved technologies in comparison to farmers’ practices (Rs. 38775/ha). The frontline demonstrations conducted on green gram at the farmers’ field revealed that the adoption of improved technologies significantly increased the yield as well as yield attributing traits of crop and also the net returns higher than the farmers’ practices. So, there is a need to disseminate the improved technologies among the farmers with effective extension methods like training and demonstrations. The farmers’ should be encouraged to adopt the recommended package of practices realizing for higher returns

ROMK expression remains unaltered in a mouse model of familial hyperkalemic hypertension caused by the CUL3Δ403-459 mutation.

Familial hyperkalemic hypertension (FHHt) is a rare inherited form of salt-dependent hypertension caused by mutations in proteins that regulate the renal Na(+)-Cl(-) cotransporter NCC Mutations in four genes have been reported to cause FHHt including CUL3 (Cullin3) that encodes a component of a RING E3 ligase. Cullin-3 binds to WNK kinase-bound KLHL3 (the substrate recognition subunit of the ubiquitin ligase complex) to promote ubiquitination and proteasomal degradation of WNK kinases. Deletion of exon 9 from CUL3 (affecting residues 403-459, CUL3(Δ403-459)) causes a severe form of FHHt (PHA2E) that is recapitulated closely in a knock-in mouse model. The loss of functionality of CUL3(Δ403-459) and secondary accumulation of WNK kinases causes substantial NCC activation. This accounts for the hypertension in FHHt but the origin of the hyperkalemia is less clear. Hence, we explored the impact of CUL3(Δ403-459) on expression of the distal secretory K channel, ROMK, both in vitro and in vivo. We found that expressing wild-type but not the CUL3(Δ403-459) mutant form of CUL3 prevented the suppression of ROMK currents by WNK4 expressed in Xenopus oocytes. The mutant CUL3 protein was also unable to affect ROMK-EGFP protein expression at the surface of mouse M-1 cortical collecting duct (CCD) cells. The effects of CUL3 on ROMK expression in both oocytes and M-1 CCD cells was reduced by addition of the neddylation inhibitor, MLN4924. This confirms that neddylation is important for CUL3 activity. Nevertheless, in our knock-in mouse model expressing CUL3(Δ403-459) we could not show any alteration in ROMK expression by either western blotting whole kidney lysates or confocal microscopy of kidney sections. This suggests that the hyperkalemia in our knock-in mouse and human PHA2E subjects with the CUL3(Δ403-459) mutation is not caused by reduced ROMK expression in the distal nephron.British Heart Foundation. Grant Number: PG/13/89/30577This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.14814/phy2.1285

Effect of pre-harvest spray of calcium nitrate, boric acid and zinc sulphate on storability of Nagpur mandarin (Citrus reticulata Blanco)

An investigation was carried out at Fruit Research Farm, Department of Fruit Science at College of Horticulture and Forestry, Jhalawar during 16 September, 2014 to 1 March, 2015 to study the individual effect of Pre-harvest spray of Calcium nitrate, Zinc sulphate and Boric acid as well as their interaction on Storability of Nagpur mandarin (Citrus reticulata Blanco) after fruit set stage. Among different pre-harvest treatments, T27 treatment (Ca 3.0% + B 0.6% + Zn 0.6%) treated fruits had the longest shelf-life of 15 days at ambient conditions with lowest PLW (9.27 %), decay per cent (15.23 %) and better organoleptic ratting (8.41/10) and higher retention of juice percent (39.12 %) till end of storage period

Taxonomic studies of Eulophid parasitoids (Hymenoptera: Chalcidoidea) collected from Uttarakhand, India

A sound taxonomic knowledge base is a prerequisite for effective conservation, environment assessment, ecological research, management and sustainable use of biological resources. Parasitoids are the major component of the biocontrol, so the correct identification of the parasitoid is very important task. Eulophidae is a large family of the superfamily Chalcidoidea and comprises promising biocontrol agents for the control of insect pests causing harm to agricultural ecosystem. The present study has been done to provide the account and occurrence of 4 genera belonging to subfamily Entedoninae, Eulophinae and Tetrastichinae. Entedon costalis Dalman, Diglyphus horticola Khan, Hemiptarsenus varicornis (Girault), and Neotrichoporoides viridimaculatus (Fullaway) was collected from Uttarakhand (India) and described in detail with additional morphological characters that aids in clear identification of the parasitoids. Entedon costalis is recorded from this region with additional characters for identification

Removal of hexavalent chromium Cr (VI) using activated carbon prepared from mango kernel activated with H3PO4

The present work reported the adsorption of Cr (VI) from aqueous solutions on activated carbon prepared from mango kernel, a seasonal waste from mango fruits. Kernels from dried mango fruit shells were taken out and pulverized in a micro-pulverizing mill. The powder thus obtained was activated with 40% H3PO4 and carbonized at 600 °C for 1 hour in an inert atmosphere. Physico-chemical characteristics such as elemental composition, surface area, functional groups and surface morphology of the activated carbon were analyzed using elemental analyzer, BET surface area analyzer, FTIR spectroscopy and SEM analysis respectively. Batch adsorption experiments were performed to investigate the effects of Cr (VI) concentration, carbon dose, pH, rate of agitation, time and temperature. The maximum adsorption capacity of Cr(VI) was found to be 7.8 mg g−1 at pH 2 and temperature 35 °C. The Langmuir adsorption isotherm best represented the equilibrium data and a pseudo-second order relation represented the adsorption kinetics

Lineage dynamics of murine pancreatic development at single-cell resolution.

Organogenesis requires the complex interactions of multiple cell lineages that coordinate their expansion, differentiation, and maturation over time. Here, we profile the cell types within the epithelial and mesenchymal compartments of the murine pancreas across developmental time using a combination of single-cell RNA sequencing, immunofluorescence, in situ hybridization, and genetic lineage tracing. We identify previously underappreciated cellular heterogeneity of the developing mesenchyme and reconstruct potential lineage relationships among the pancreatic mesothelium and mesenchymal cell types. Within the epithelium, we find a previously undescribed endocrine progenitor population, as well as an analogous population in both human fetal tissue and human embryonic stem cells differentiating toward a pancreatic beta cell fate. Further, we identify candidate transcriptional regulators along the differentiation trajectory of this population toward the alpha or beta cell lineages. This work establishes a roadmap of pancreatic development and demonstrates the broad utility of this approach for understanding lineage dynamics in developing organs