Evaluation of dredged material proposed for ocean disposal from MOTBY

The National Park Service, US Department of the Interior requested U.S. Army Corps of Engineers/New York District (USACE-NYD) to evaluate sediments around the Military Ocean Terminal (MOTBY) in Bayonne, New Jersey for unconfined ocean disposal at the Mud Dump Site. Sediment samples were collected from MOTBY. Tests and analyses were conducted on MOTBY sediment core samples. The evaluation of proposed dredged material from MOTBY included grain size and total organic carbon (TOC) analyses and one acute toxicity test with the amphipod Ampelisca abdita. In addition to this benthic toxicity test, a bioaccumulation test (28-day exposure) was conducted

Breaking the Silence: Achieving a Positive Campus Climate for Diversity from the Staff Perspective

The purpose of this paper is to identify factors that create a positive climate for diversity and to demonstrate how these factors predict outcomes related to achieving a positive campus climate for diversity. Based on survey data collected from 437 staff members employed at a large, public, predominantly White university in the Midwest, results suggest that the institution’s ability to achieve a positive climate for diversity reflects not only the personal characteristics of the staff member (race, gender, education level, and age) but also their perceptions of their immediate work environment. Implications are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43633/1/11162_2004_Article_8152.pd

First-year LGBQ+ students in higher education: who are they and where do they enroll?

Recent decades have seen a growing body of scholarship dedicated to the college experiences of lesbian, gay, bisexual, and queer (LGBQ+) students. To date, research on LGBQ+ students offers much-needed insight into their postsecondary experiences and outcomes (Rankin et al., 2019), and large-scale surveys indicate the proportion of LGBQ+ students is on the rise (e.g., Eagan et al., 2015; 2017). Yet, other than a breakdown of the types of institutions at which LGBQ+ students enroll (e.g., public and private 4-year colleges and universities) (Eagan et al., 2015; 2017), there is no national snapshot of this group’s demographic characteristics. Further, comparatively little quantitative research on this population exists (Garvey, 2014), with only one national survey, the American College Health Association (ACHA) National College Health Assessment, consistently collecting data on LGBQ+-identified students for more than a decade. Given the need for a more comprehensive understanding of the LGBQ+ collegiate community and the implications of such insight, the purpose of this study is twofold. First, it provides an estimate of the first-year LGBQ+ college student population at specific institution types using national data from entering college students. Second, it yields new information on the characteristics of first-year LGBQ+ individuals in United States higher education. METHODS: Data for this study come from the Interfaith Diversity Experiences and Attitudes Longitudinal Survey (IDEALS). IDEALS—a national, longitudinal study of students’ experiences with and perspectives toward religious, worldview, and other types of diversity (including sexual orientation)—was first fielded in the fall of 2015 to entering students at 122 U.S. college and universities who were recruited based on a national sampling frame that accounted for geography, size, control, and selectivity. Of the 122 institutions, 32 are public (41% of respondents), 32 are Protestant-affiliated (21% of respondents), 29 are private nonsectarian (24% of respondents), 15 are Evangelical Protestant (7% of respondents), and 14 are Catholic-affiliated (8% of respondents). A total of 20,436 students participated in the first wave of data collection; however, data on sexual orientation were only collected from 121 colleges, as one institution opted to omit the sexual orientation question given concerns for student privacy. Additional tests confirmed the exclusion of this institution had no impact on the findings. [End Page 499] White women were overrepresented in the unweighted dataset, while Men of Color were underrepresented. In light of representation issues and to ensure nationally representative results, the dataset was weighted using the “Generalized Raking Method” (see Deville et al. [1993] for an overview of the method). The idea of this approach is to come up with adjustment factors so that the percentages found in a survey match a set of known population percentages. Using computer code publicly available through the French National Institute of Statistics and Economic Studies (INSEE) (INSEE, 2018), weights were constructed to match the Integrated Postsecondary Education Data System (IPEDS) fall 2015 census of postsecondary enrollment on six dimensions: sex1 (female and male); race/ethnicity (Students of Color and white students); institutional status (private religious, private nonreligious, and public); Carnegie classification (large research or many doctoral programs, many master’s degree programs, and primarily bachelor’s or associate’s degree); geographic region (Northeast, Midwest, South, and West), and urbanicity (urban/city-set institution and not-city-set institution). As a reliable, standardized, and transparent data source vetted by the National Center of Education Statistics, IPEDS reduces both biases and missing data potentials. Unless noted, all tables presented in the results are weighted according to conventional weighting practices (Deville et al., 1993). ANALYSES AND RESULTS: We conducted descriptive analyses to examine how many LGBQ+ first-year students enroll at U.S. four-year postsecondary institutions. Of the approximately 3.2 million students who entered college in 2015, the vast majority identified as heterosexual (86.2%, n = 2,775,399). However, with 13.8% of students identifying as LGBQ+ (n = 444,862), this study suggests that the percentage of LGBQ+ students on U.S. campuses is higher than...First author draf

Inclusive growth in English cities: mainstreamed or sidelined?

<p>The concept of inclusive growth is increasingly presented as offering prospects for more equitable social outcomes. However, inclusive growth is subject to a variety of interpretations and lacks definitional clarity. In England, via devolution, cities are taking on new powers for policy domains that can influence inclusive growth outcomes. This opens up opportunities for innovation to address central issues of low pay and poverty. This paper examines the extent to which inclusive growth concerns form a central or peripheral aspect in this new devolution through the content analysis of devolution agreements. It concludes that inclusive growth concerns appear to be largely sidelined.</p

Evaluation of Dredged Material Proposed for Ocean Disposal from Port Chester, New York

Port Chester was one of seven waterways that the US Army Corps of Engineers-New York District requested the Battelle Marine Sciences Laboratory to sample and evaluate for dredging and disposal in March 1994. Tests and analyses were conducted on Port Chester sediment core samples. Because the Port Chester area is located on the border between New York and southeast Connecticut, its dredged material may also be considered for disposal at the Central Long Island Sound Disposal Site. The sediment evaluation consisted of bulk sediment chemical analyses, chemical analyses of site water and dredged material elutriate preparations, water-column and benthic acute toxicity tests, and bioaccumulation studies. Individual sediment core samples collected from Port Chester were analyzed for grain size, moisture content, and total organic carbon. In addition, sediment was analyzed for bulk density, specific gravity, metals, chlorinated pesticides, polychlorinated biphenyl congers, polynuclear aromatic hydrocarbons and 1,4-dichlorobenzene

Longitudinal changes in respiratory and upper limb function in a pediatric type III spinal muscular atrophy cohort after loss of ambulation

Introduction/Aims: Spinal muscular atrophy (SMA) type III is a relatively mild form of SMA. Few studies have investigated the changes in both respiratory and upper limb function within this population after loss of ambulation. The aim of this study was to assess change in percentage of predicted forced vital capacity (FVC% predicted) and change in the Revised Upper Limb Module (RULM) score in these patients throughout a 24-month period after loss of ambulation. Effect of scoliosis and its surgical correction, disease duration since loss of ambulation, weight, and height were also investigated. / Methods: Retrospective analyses were performed on 24 nonambulant SMA III patients from data collected at two centers in the United Kingdom. / Results: The FVC% predicted score showed a significant progressive deterioration of 17% over the 24-month period. Respiratory deterioration correlated significantly with age, weight, disease duration since loss of ambulation, and spinal correctional surgery. Longitudinal RULM data were available for 16 patients; a significant deterioration was observed with a mean decrease in score of 3 over 24 months. Age correlated negatively with RULM score, as did height and time since loss of ambulation. A significant positive correlation between FVC% predicted and RULM was demonstrated. / Discussion: This study highlights how SMA type III patients have progressive deterioration of respiratory and upper limb function after loss of ambulation. Combining data from these assessments could provide insight into clinical progression, inform clinical trials, and provide assistance in managing disease progression expectations for patients

Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review

Quantitative immunoelectron microscopy uses ultrathin sections and gold particle labelling to determine distributions of molecules across cell compartments. Here, we review a portfolio of new methods for comparing labelling distributions between different compartments in one study group (method 1) and between the same compartments in two or more groups (method 2). Specimen samples are selected unbiasedly and then observed and expected distributions of gold particles are estimated and compared by appropriate statistical procedures. The methods can be used to analyse gold label distributed between volume-occupying (organelle) and surface-occupying (membrane) compartments, but in method 1, membranes must be treated as organelles. With method 1, gold counts are combined with stereological estimators of compartment size to determine labelling density (LD). For volume-occupiers, LD can be expressed simply as golds per test point and, for surface-occupiers, as golds per test line intersection. Expected distributions are generated by randomly assigning gold particles to compartments and expressing observed/expected counts as a relative labelling index (RLI). Preferentially-labelled compartments are identified from their RLI values and by Chi-squared analysis of observed and expected distributions. For method 2, the raw gold particle counts distributed between compartments are simply compared across groups by contingency table and Chi-squared analysis. This identifies the main compartments responsible for the differences between group distributions. Finally, we discuss labelling efficiency (the number of gold particles per target molecule) and describe how it can be estimated for volume- or surface-occupiers by combining stereological data with biochemical determinations

High-throughput functional analysis of CFTR and other apically localized proteins in iPSC-derived human intestinal organoids

Induced Pluripotent Stem Cells (iPSCs) can be differentiated into epithelial organoids that recapitulate the relevant context for CFTR and enable testing of therapies targeting Cystic Fibrosis (CF)-causing mutant proteins. However, to date, CF-iPSC-derived organoids have only been used to study pharmacological modulation of mutant CFTR channel activity and not the activity of other disease-relevant membrane protein constituents. In the current work, we describe a high-throughput, fluorescence-based assay of CFTR channel activity in iPSC-derived intestinal organoids and describe how this method can be adapted to study other apical membrane proteins. Specifically, we show how this assay can be employed to study CFTR and ENaC channels and an electrogenic acid transporter in the same iPSC-derived intestinal tissue. This phenotypic platform promises to expand CF therapy discovery to include strategies that target multiple determinants of epithelial fluid transport

A Nonsynonymous Change in Adhesion G Protein–Coupled Receptor L3 Associated With Risk for Equine Degenerative Myeloencephalopathy in the Caspian Horse

Equine degenerative myeloencephalopathy (EDM), a neurological disease of young horses, causes progressive development of symmetric ataxia predominantly in the pelvic limbs. Equine degenerative myeloencephalopathy is likely inherited and with no known treatment affected horses frequently need euthanasia. Alpha-tocopherol deficiency during early life appears to contribute to the phenotype. This study sought to identify any genetic variants correlated with EDM in Caspian foals. Two half-sibling EDM-diagnosed cases were genotyped at 52,063 loci and evaluated by the Autozygosity by Difference statistic. Additional horses not affected by EDM were used for genetic comparison to identify regions unique to the case phenotype. The associated region on chromosome 3 contains only one gene encoding adhesion G protein–coupled receptor L3 (ADGRL3). Adhesion G protein–coupled receptor L3 is a member of the latrophilin subfamily of G protein–coupled receptors and may contribute to attention deficit/hyperactivity disorder in humans and hyperactive motor function in mice and zebrafish. Analysis of the predicted coding regions for Equine ADGRL3 in affected horses revealed a nonsynonymous single nucleotide polymorphism at Chr3:71,917,591 bp. Caspian and Caspian cross-relatives (n = 81) of the two initial cases and unrelated horses from similar breeds (n = 130, including Arabians, American Miniatures, and Shetlands) possessed this allele at 5% frequency, with no homozygotes observed within the non-Caspian breeds. This study suggests that a polymorphism in ADGRL3 could contribute to a genetic predisposition to Caspian horse EDM

Novel 3D Microscopic Analysis of Human Placental Villous Trees Reveals Unexpected Significance of Branching Angles

The villous trees of human placentas delineate the fetomaternal border and are complex three-dimensional (3D) structures. Thus far, they have primarily been analyzed as thin, two-dimensional (2D) histological sections. However, 2D sections cannot provide access to key aspects such as branching nodes and branch order. Using samples taken from 50 normal human placentas at birth, in the present study we show that analysis procedures for 3D reconstruction of neuronal dendritic trees can also be used for analyzing trees of human placentas. Nodes and their branches (e.g., branching hierarchy, branching angles, diameters, and lengths of branches) can be efficiently measured in whole-mount preparations of isolated villous trees using high-end light microscopy. Such data differ qualitatively from the data obtainable from histological sections and go substantially beyond the morphological horizon of such histological data. Unexpectedly, branching angles of terminal branches of villous trees varied inversely with the fetoplacental weight ratio, a widely used clinical parameter. Since branching angles have never before been determined in the human placenta, this result requires further detailed studies in order to fully understand its impact