Evaluation of Intracellular Ca2+ Concentration by Fura 2 Ratiometry in Encystment-induced Colpoda cucullus

In Colpoda cucullus, the signaling pathways for encystment induction involving protein phosphorylation have been believed to be triggered by an increase in the intracellular Ca2+ concentration promoted by cell-to-cell mechanical contact due to overpopulation. By means of fura 2 ratiometry, the present study showed that the intracellular Ca2+ concentration was actually elevated when vegetative cells were induced to encyst by being suspended at a high cell density in the presence of external free Ca2+ and suppressed by chelating external Ca2+. This result strongly suggests that an increase in the intracellular Ca2+ concentration caused by an inflow of Ca2+ promoted by cell-tocell mechanical contact due to overpopulation enhances the rate of encystation in Colpoda cucullus

Studies on the photoresponses in Blepharisma japonicum

Thesis--University of Tsukuba, D.Sc.(B), no. 303, 1986. 3. 2

Photoresponse in the Ciliated Protozoan Colpoda cucullus

Kawano, Noriyuki, Funadani, Ryoji, Arikawa, Mikihiko, Harada, Tetsuo, Suizu, Futoshi, Matsuoka, Kou, Matsuoka, Tatsuomi (2017): Photoresponse in the Ciliated Protozoan Colpoda cucullus. Acta Protozoologica 56 (1): 1-7, DOI: 10.4467/16890027AP.17.001.6965, URL: https://www.mendeley.com/catalogue/0cd0b706-68e1-3ba9-9e25-c545a0f41b65

Evaluation of Intracellular Ca2+ Concentration by Fura 2 Ratiometry in Encystment-induced Colpoda cucullus

In Colpoda cucullus, the signaling pathways for encystment induction involving protein phosphorylation have been believed to be triggered by an increase in the intracellular Ca2+ concentration promoted by cell-to-cell mechanical contact due to overpopulation. By means of fura 2 ratiometry, the present study showed that the intracellular Ca2+ concentration was actually elevated when vegetative cells were induced to encyst by being suspended at a high cell density in the presence of external free Ca2+ and suppressed by chelating external Ca2+. This result strongly suggests that an increase in the intracellular Ca2+ concentration caused by an inflow of Ca2+ promoted by cell-tocell mechanical contact due to overpopulation enhances the rate of encystation in Colpoda cucullus

Step-Up and Step-Down Photoresponses in Blepharisma(Physology)

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