Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: Case report

In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time. © Human stem cells institute, 2013

Reaction of oligoglia to spinal cord injury in rats and transplantation of human olfactory ensheathing cells

In experiments on rats with lateral TVIIIhemisection of the spinal cord and transplantation of ensheating olfactory cells, we studied structural changes at the lesion site and adjacent rostral and dorsal regions of the spinal cord. The state of oligodendrocytes and myelin fibers and motor function in experimental animal were analyzed. Open field testing (BBB test) showed that motor functions steadily increased (by 13% on average) within the interval from day 21 to day 53 after transplantation. Histological examination showed that groups of transplanted cells carrying human nuclear marker (HNu + cells) were still present at the lesion site 30 days after surgery. Some of these cells migrated in the rostral and caudal directions from the injection site to a distance up to 6 mm. At the initial period after hemisection, the number of oligodendrocytes (O4+-cells) in the immediate vicinity to the lesion site decreased 2-fold, but no signifi cant changes in the number of neurons were found in the rostral and dorsal fragments of the spinal cord compared to the corresponding parameter in controls. Sixty days after transplantation, the cross-section area in the rostral part of the spinal cord at a distance of 3 mm from damage site increased by 15.3% compared to the control. The number of O4+- cells at the lesion site and in adjacent rostral and caudal parts of the spinal cord by 22.8% surpassed that in the control group. The number of remyelinated axons also increased. These findings suggest the absence of pronounced structural changes in the rostral and caudal parts of the spinal cord compared to lesion site at early stages after damage and cell transplantation. At the same time, pronounced activation of oligodendrocytes in this region suggests their involvement together with Schwann cells into remyelination of regenerating axons, which can serve as a factor of partial restoration of motor functions after spinal cord injury. © 2010 Springer Science+Business Media, Inc

Application of Allogenic Adipose-Derived Multipotent Mesenchymal Stromal Cells from Cat for Tibial Bone Pseudoarthrosis Therapy (Case Report)

© 2016, Springer Science+Business Media New York.A clinical case reported here demonstrates the possibility of using the multipotent mesenchymal stromal cells obtained from the donor animal to stimulate the process of bone formation in surgical treatment of tibia pseudoarthrosis in the cat. The cells were isolated from adipose tissue of the donor cat and express on their surface main markers of mesenchymal stromal cells—CD 44 and Thy-1. As a result of manipulation, the bone regenerate was formed and the support function of the injured limb was restored

Use of stromal vascular fraction of adipose tissue for treatment of femur pseudoarthtosis: Case report

The article presents a case report of successful treatment of pseudarthrosis of the femur with the use of autologous cells of the stromal vascular fraction (SVF) derived from adipose tissue. Autologous SVF cells mixed with fibrin glue were transplanted into bone defect by injection under the control of electro-optical converter. Two months after injection, the patient reported the disappearance of pain. After 4 months we observed the recovery of function of the knee, X-ray shows signs of the fracture healing through the formation of bone callus. © Human stem cells institute, 2013

Considerations and strategies in L2 vocabulary acquisition among Japanese 1st year university students.

This article proposes a framework of strategies1） for L2 vocabulary acquisition among low-to-mid level L2 learners in their first year at Kansai University. The framework relies upon considerations posited by Kudo (1999), as well as, Hunt and Beglar’s (2005) model for developing EFL reading vocabulary, although the objective for our target learners is tofacilitate vocabulary acquisition in a learner-centered communicative context, where possible. This paper firstly underlines the critical role of vocabulary in second language acquisition, while raising awareness of the surrounding pedagogic climate in Japanese secondary education.研究ノー

Influence of small businesses on the competitive development of the Volga Federal District regions

© 2016 Taylor & Francis Group, London.The article examines the impact of small businesses on the main indicators of the development of Volga Federal District (VFD) regions for the period from the year 2002 to 2012. As a result, the regression analysis has statistically revealed a significant association between the indicators of small business development and the performance of the VFD

Genetic modification of adipose derived stem cells with recombinant plasmid DNA pBud-VEGF-FGF2 results in increased of IL-8 and MCP-1 secretion

Bacterial plasmid DNA is often used in gene therapy application as a vector for delivering recombinant genes into eukaryotic cells. In this work we study effect of plasmid DNA encoding pro-angiogenic growth factors VEGF and FGF2 on cytokine production of human adipose derived stem cells (ADSC). Adipose tissue contain progenitor and stem cells with angiogenic potential which are considered promising cell source for treating ischemic conditions. Genetic modification of human ADSC with recombinant plasmids encoding therapeutic growth factors might change therapeutic potential of these cells. We report that genetic modification of ADSC with recombinant plasmid pBud-VEGF-FGF2 results in expected increased of VEGF secretion and also results in increase of IL-8 and MCP-1 secretion into culture medium

Use of Gene-Activated Demineralized Bone Allograft in the Therapy of Ulnar Pseudarthrosis. Case Report

© 2016, Springer Science+Business Media New York.This paper presents a clinical case of successful ulnar pseudarthrosis treatment using a gene-activated bone allograft containing VEGF (vascular endothelial growth factor) and BMP2 (bone morphogenetic protein 2) in the form of a multicystron plasmid. Demineralized bone matrix with applied recombinant plasmid DNA was grafted into the bone defect using the classical open surgical approach. Two months after the surgery, the patient noticed the disappearance of pain including pain during activity. On X-rays of ulna, signs of union in the form of callus formation were found

Surgical Procedure for Extracting Pig Teeth for Isolation and Cultivation of Mesenchymal Stem Cells from Dental Pulp for Regenerative Therapy Applications

© 2016, Springer Science+Business Media New York.Mesenchymal stem cells (MSCs) are considered the most versatile cells for cell therapy—particularly for repair of injuries to the central nervous system. Recently, the use of dental pulp MSCs (DP-MSCs) for spinal cord regeneration has became especially important. We describe a surgical procedure for extracting pig teeth to obtain DP-MSCs using protocols for direct and enzymatic isolation of DP-MSCs followed by cultivation. Our study shows that primary pulp cultures of DP-MSCs are established 5 days after enzymatic digestion and 7–10 days following attachment of the digested minced tissue to the bottom of a plate. Though in the first few days the rate of primary expansion for cultures generated by direct isolation was lower than the rate of enzymatic digestion, this difference leveled off on days 14–18 of culture. For DP-MSCs isolation, we recommend the use of deciduous succedaneous lateral incisors and canine teeth of pigs as well as deciduous premolars from the first dentition of young pigs (up to 3 months)

Stimulation of rat's sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelial growth factor and basic fibroblast growth factor

The development of effective treatments for patients with peripheral nerve injury is an urgent task of biomedicine. «Gold» standard in restoring the integrity of nerve conduits is auto-nerve transplantation in which a peripheral nerve defect is corrected with autologous nerve graft. Here we propose a method for stimulating revascularization and regeneration of auto-nerve graft by a local injection of plasmid pBud-VEGFFGF2, expressing vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF2). It is shown that direct injection of plasmid pBud-VEGF-FGF2 in the proximal and distal segments of nerve, as well as in the auto-nerve graft, stimulates the regeneration of the rat's sciatic nerve and restores motor activity