Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in -0

In green the melt curve profile (in triplicate) of mutant clone PS-Peru is shown with a Tm of of 51.9°C. The change in amount of fluorescence for each probe-template hybrid was plotted against the temperature and its negative derivative appeared as a positive peak. The grey lines represent the negative controles.<p><b>Copyright information:</b></p><p>Taken from "Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in "</p><p>http://www.malariajournal.com/content/7/1/48</p><p>Malaria Journal 2008;7():48-48.</p><p>Published online 17 Mar 2008</p><p>PMCID:PMC2276220.</p><p></p

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in -1

Ted in blue and purple are shown. The sample represented in purple contains according to the intergrated software a 45% to 55% ratio of mutant and wild type infection respectively. The sample represented in blue shows the opposite pattern and contains 55% to 45% mutant and wild type infection respectively. This can also be seen in the figure by the difference in peak hight. The red curves are the positive controls for wild type strains and the green for mutant strains. The blue horizontal line represents the threshold for background fluorescence, and the curve entirely below shows the results for the negative control (gray).<p><b>Copyright information:</b></p><p>Taken from "Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in "</p><p>http://www.malariajournal.com/content/7/1/48</p><p>Malaria Journal 2008;7():48-48.</p><p>Published online 17 Mar 2008</p><p>PMCID:PMC2276220.</p><p></p

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in -3

Ted in blue and purple are shown. The sample represented in purple contains according to the intergrated software a 45% to 55% ratio of mutant and wild type infection respectively. The sample represented in blue shows the opposite pattern and contains 55% to 45% mutant and wild type infection respectively. This can also be seen in the figure by the difference in peak hight. The red curves are the positive controls for wild type strains and the green for mutant strains. The blue horizontal line represents the threshold for background fluorescence, and the curve entirely below shows the results for the negative control (gray).<p><b>Copyright information:</b></p><p>Taken from "Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in "</p><p>http://www.malariajournal.com/content/7/1/48</p><p>Malaria Journal 2008;7():48-48.</p><p>Published online 17 Mar 2008</p><p>PMCID:PMC2276220.</p><p></p

Performance of smear-microscopy using Ziehl Neelsen and LED-fluorescence microscopy using two smear-positive case definitions.

<p>ZN: Ziehl Neelsen, LED-FM: Light Emitting Diode Fluorescence Microscopy.</p

Study profile.

<p>Study profile.</p

Smear microscopy results and smear microscopic appearance per microscopy methods.

<p>NA  =  non applicable.</p>1<p>1 length = 100 HPF 1000× magnification (ZN) and 200 HPF under 400× magnification (FM).</p>2<p>3 slides broken.</p><p>ZN: Ziehl Neelsen; LED-FM: light-emitting diode fluorescence microscopy.</p

Patients' characteristics.

<p>Patients' characteristics.</p

Results of smear reading using LED-FM and conventional FM (N = 332).

<p>LED-FM: Light Emitting Diodes Fluorescence microscopy.</p

Performance of the LED-FM after NaOCl sedimentation using a case definition of one positive smear out of two collected specimens and different AFB cut-offs to define a positive smear.

<p>Performance of the LED-FM after NaOCl sedimentation using a case definition of one positive smear out of two collected specimens and different AFB cut-offs to define a positive smear.</p

Study profile.

<p>ICF: Informed Consent Form, ZN: Ziehl Neelsen, LED-FM: Light Emitting Diode Fluorescence Microscopy; MTB: <i>Mycobacterium tuberculosis</i>; MOTT: Mycobacterium Other Than Tuberculosis.</p