In green the melt curve profile (in triplicate) of mutant clone PS-Peru is shown with a Tm of of 51.9°C. The change in amount of fluorescence for each probe-template hybrid was plotted against the temperature and its negative derivative appeared as a positive peak. The grey lines represent the negative controles.<p><b>Copyright information:</b></p><p>Taken from "Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in "</p><p>http://www.malariajournal.com/content/7/1/48</p><p>Malaria Journal 2008;7():48-48.</p><p>Published online 17 Mar 2008</p><p>PMCID:PMC2276220.</p><p></p
