Materiali per le pavimentazioni dei ricoveri ovini.

The AA. illustre a study on different flooring for sheep housing, they comparing all the types according to shape and/or emploid material, functional performances, comfort, resistance and cost. Are also indicated some solutions whose utilization is expected to be remarkably convenient in prospective of breeding techinques and methods, at present developing to mroe intensive and specialized form

Lithiation of 4-membered heterocycles as useful strategy for the preparation of new molecular scaffolds: addressing the regioselectivity in azetidines and thietanes

Four-membered heterocycles (4-MH) with one or two heteroatoms are of great importance in medicinal chemistry and synthetic organic chemistry. This kind of scaffolds show peculiar structural features, related to the ring “puckering”, and biological properties. Our recent research efforts have been focused on the stereoselective synthesis and functionalization of some 4-MH such as azetidines, thietanes and oxazetidines

Origin and distribution of the BRCA2-8765delAG mutation in breast cancer

Background: The BRCA2-8765delAG mutation was firstly described in breast cancer families from French-Canadian and Jewish-Yemenite populations; it was then reported as a founder mutation in Sardinian families. We evaluated both the prevalence of the BRCA2-8765delAG variant in Sardinia and the putative existence of a common ancestral origin through a haplotype analysis of breast cancer family members carrying such a mutation. Methods: Eight polymorphic microsatellite markers (D13S1250, centromeric, to D13S267, telomeric) spanning the BRCA2 gene locus were used for the haplotype analysis. Screening for the 8765delAG mutation was performed by PCR-based amplification of BRCA2-exon 20, followed by automated sequencing. Results: Among families with high recurrence of breast cancer (≥ 3 cases in first-degree relatives), those from North Sardinia shared the same haplotype whereas the families from French Canadian and Jewish-Yemenite populations presented distinct genetic assets at the BRCA2 locus. Screening for the BRCA2-8765delAG variant among unselected and consecutively-collected breast cancer patients originating from the entire Sardinia revealed that such a mutation is present in the northern part of the island only [9/648 (1.4%) among cases from North Sardinia versus 0/493 among cases from South Sardinia]. Conclusion: The BRCA2-8765delAG has an independent origin in geographically and ethnically distinct populations, acting as a founder mutation in North but not in South Sardinia. Since BRCA2- 8765delAG occurs within a triplet repeat sequence of AGAGAG, our study further confirmed the existence of a mutational hot-spot at this genomic position (additional genetic factors within each single population might be involved in generating such a mutation)

Geologic-geomorphologic evolution of the Río Areco basin, north-eastern of Buenos Aires province

La cuenca del río Areco integra la red de drenaje de la Pampa Ondulada, NE de la provincia de Buenos Aires. Los procesos geomórficos marinos, fluvio-lacustres y eólicos actuaron sobre los sedimentos loéssicos y loessoides de la Formación Pampeano (Pleistoceno) dejando, con diferentes grados de desarrollo, el registro sedimentario del Pleistoceno tardío y Holocenoa lo largo de toda la cuenca. En estos depósitos se han reconocido, al menos, dos episodios pedogenéticos. Edades 14C sobre MO de estos paleosuelos arrojaron valores de 7.000 ± 240 y 1.940 ± 80 años AP en San Antonio de Areco y 2.320 ± 90 y 2.000 ± 90 años AP en Puente Castex, para dos importantes estabilizaciones del paisaje, separadas en esta última localidad por un breve episodio de sedimentación. La cuenca inferior en la cañada Honda, fue ocupada por la ingresión durante MIS 1 (Formación Campana), dejando un amplio paleoestuario limitado por acantilados. El retroceso de la línea de costa y la continentalización del ambiente permitió la formación de un suelo datado en 3.070 ± 90 años 14C AP cubierto por sedimentos aluviales. Los sedimentos litorales son en general de baja energía, asociados a tres litofacies diferentes, cuyas edades fluctúan entre los 4.270 ± 70 años 14C AP en esta cuenca y 6.000 ± 80 14C años AP, 6.370 ± 90 años 14C AP, 3.640 ± 70 años 14C AP,5.630 ± 100 años 14C AP y 5.420 ± 110 años 14C AP, en cuencas aledañas. La ingresión durante MIS 5e también entró por el río Areco evidenciando ya la existencia de esta depresión. El límite externo de esta cuenca lo constituye el delta del Paraná cuya progradación ha dejado expuesto morfologías y depósitos de ambientes fluviales, observándose que el contacto entre ambas unidades morfológicas (delta y paleoestuario) está determinado por los cursos de agua, que con cambio bruscos de dirección, acompañan el sentido de avance del complejo deltaico.The Areco River Basin is part of the drainage system of Pampa Ondulada region, NE Buenos Aires Province. Geomorphic processes that it formed, acted on the loessoid sediments of the Pampeano Formation (Pleistocene) building a morphology contrasting with those of the surrounding environment. In the upper and middle basin there are fluvial successions (Late Pleistocene-Holocene) with different development of pedogenesis. 14C on OM of these paleosoils yielded 7,000 ± 240 and 1,940 ± 80 years BP in San Antonio de Areco, and 2,320 ± 90 and 2,000 ± 90 years 14C BP in Castex Bridge, for two important landscape stabilizations, separated in the latter place for a brief episode of sedimentation. The lower basin, together with Cañada Honda locality, was occupied during MIS 1 ingression (Campana Formation) leaving a wide paleoestuary, limited by fossil cliffs. These sediments were generally deposited in low energy environments, associated with three different lithofacies; 14C ages range between 4,270 ± 70 years BP in this basin and 6,000 ± 80, 6,370 ± 90, 3,640 ±70, 5,630 ± 100, and 5,420 ±,110 years BP in near basins. Based on this information, the late Pleistocene ingression MIS 5e also entered by Areco River putting in evidence, already, the existence of this depression. The external limit of this unit is the Paraná Delta River whose progradation exposed specific morphologies and deposits. In the contact between both morphological units (delta and paleoestuary) is determined by the water streams that, with abrupt direction changes according to deltaic complex development.Fil: Fucks, Enrique Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Blasi, Adriana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Mineralogía y Petrología; ArgentinaFil: Carbonari, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; ArgentinaFil: Huarte, Roberto Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; ArgentinaFil: Pisano, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Facultad de Ciencias Naturales y Museo, Universidad Nacional de la Plata; ArgentinaFil: Aguirre, Marina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Facultad de Ciencias Naturales y Museo, Universidad Nacional de la Plata; Argentin

Attività antiproliferativa e proapoptotica di bifenili relazionabili alla struttura della curcumina su cellule di melanoma maligno

La curcumina è l’ingrediente attivo delle radici della Curcuma Longa ed è uno dei fitochimici più studiati al mondo per le sue proprietà antiossidanti, antinfiammatorie ed antitumorali. Nell'ambito della ricerca di nuovi agenti terapeutici contro il melanoma maligno (MM) abbiamo caratterizzato l’attività antiproliferativa sia della curcumina (D1) che di alcuni bifenili, strutturalmente riconducibili ad essa, su colture cellulari primarie di MM

2,2'-diidrossi-3,3'-dimetossi-5,5'-dimetil-6,6'-dibromo-1,1'-bifenile: preparazione, risoluzione, struttura e attività biologica

Nell'ambito dei nostri studi riguardanti la sintesi di nuovi sistemi idrossilati bifenilici come builing blocks per la preparazione sia di potenziali agenti farmaceutici che di nuovi leganti, abbiamo individuato nel 2,2'-diidrossi-3,3'-dimetossi-5,5'-dimetil-1,1'- bifenile (deidrodicreosolo) un possibile precursore di derivati 6,6'-dibromo disostituiti conformazionalmente stabili

New pieces in the transmission cycle of the hepatitis E virus in South America: First viral detection in wild boars from Argentina

The hepatitis E virus (HEV) causes hepatitis worldwide. In Europe, wild boars are known to be viral reservoirs and sources of infection. In South America, there is a lack of information about HEV in these animals. A total of 102 wild boar serum samples from Argentina (2014?2017) were studied for serologicalnd molecular HEV detection. We obtained a seroprevalence of 19.6%, similar to that recently described in Uruguay (the only antecedent in South America). HEV ribonucleic acid (RNA) was amplified in two anti-HEV-positive samples.Conclusions: This is the first report of HEV circulation in wild boars from Argentina, adding evidence to the findings obtained in Uruguay indicating that wild boars could be viral reservoirs in South AmericaFil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Raimondo, Natalia. Lace Laboratorios. Área Virología y Biología Molecular; ArgentinaFil: Martínez Wassaf, Maribel G.. Lace Laboratorios, Área Virología y Biología Molecular; ArgentinaFil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Outcome of cardiac surgery in patients with low preoperative ejection fraction

Background: In patients undergoing cardiac surgery, a reduced preoperative left ventricular ejection fraction (LVEF) is common and is associated with a worse outcome. Available outcome data for these patients address specific surgical procedures, mainly coronary artery bypass graft (CABG). Aim of our study was to investigate perioperative outcome of surgery on patients with low pre-operative LVEF undergoing a broad range of cardiac surgical procedures. Methods: Data from patients with pre-operative LVEF ≤40 % undergoing cardiac surgery at a university hospital were reviewed and analyzed. A subgroup analysis on patients with pre-operative LVEF ≤30 % was also performed. Results: A total of 7313 patients underwent cardiac surgery during the study period. Out of these, 781 patients (11 %) had a pre-operative LVEF ≤40 % and were included in the analysis. Mean pre-operative LVEF was 33.9 ± 6.1 % and in 290 patients (37 %) LVEF was ≤30 %. The most frequently performed operation was CABG (31 % of procedures), followed by mitral valve surgery (22 %) and aortic valve surgery (19 %). Overall perioperative mortality was 5.6 %. Mitral valve surgery was more frequent among patients who did not survive, while survivors underwent more frequently CABG. Post-operative myocardial infarction occurred in 19 (2.4 %) of patients, low cardiac output syndrome in 271 (35 %). Acute kidney injury occurred in 195 (25 %) of patients. Duration of mechanical ventilation was 18 (12-48) hours. Incidence of complications was higher in patients with LVEF ≤30 %. Stepwise multivariate analysis identified chronic obstructive pulmonary disease, pre-operative insertion of intra-aortic balloon pump, and pre-operative need for inotropes as independent predictors of mortality among patients with LVEF ≤40 %. Conclusions: We confirmed that patients with low pre-operative LVEF undergoing cardiac surgery are at higher risk of post-operative complications. Cardiac surgery can be performed with acceptable mortality rates; however, mitral valve surgery, was found to be associated with higher mortality rates in this population. Accurate selection of patients, risk/benefit evaluation, and planning of surgical and anesthesiological management are mandatory to improve outcome

Role of BRCA2 mutation status on overall survival among breast cancer patients from Sardinia

Background: Germline mutations in BRCA1 or BRCA2 genes have been demonstrated to increase the risk of developing breast cancer. Conversely, the impact of BRCA mutations on prognosis and survival of breast cancer patients is still debated. In this study, we investigated the role of such mutations on breast cancer-specific survival among patients from North Sardinia. Methods: Among incident cases during the period 1997–2002, a total of 512 breast cancer patients gave their consent to undergo BRCA mutation screening by DHPLC analysis and automated DNA sequencing. The Hakulinen, Kaplan-Meier, and Cox regression methods were used for both relative survival assessment and statistical analysis. Results: In our series, patients carrying a germline mutation in coding regions and splice boundaries of BRCA1 and BRCA2 genes were 48/512 (9%). Effect on overall survival was evaluated taking into consideration BRCA2 carriers, who represented the vast majority (44/48; 92%) of mutation-positive patients. A lower breast cancer-specific overall survival rate was observed in BRCA2 mutation carriers after the first two years from diagnosis. However, survival rates were similar in both groups after five years from diagnosis. No significant difference was found for age of onset, disease stage, and primary tumour histopathology between the two subsets. Conclusion: In Sardinian breast cancer population, BRCA2 was the most affected gene and the effects of BRCA2 germline mutations on patients' survival were demonstrated to vary within the first two years from diagnosis. After a longer follow-up observation, breast cancer-specific rates of death were instead similar for BRCA2 mutation carriers and non-carriers