PCR amplification of rRNA spacer regions was done and the shorter amplicon was excised from an agarose gel after electrophoresis at 100 V for 45 min

<p><b>Copyright information:</b></p><p>Taken from "Identification to the species level of isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling"</p><p>BMC Microbiology 2005;5():15-15.</p><p>Published online 23 Mar 2005</p><p>PMCID:PMC1079852.</p><p>Copyright © 2005 Moreira et al; licensee BioMed Central Ltd.</p> Gel purified DNA was cleaved with 11 enzymes and fragments separated by electrophoresis as before. Plus and minus signs mean positive or negative cleavage of PCR amplicon. GibcoBRL 1 kb DNA ladder was used for sizing DNA fragments and molecular masses are shown at right in bp

PCR amplification of rRNA spacer regions was done and amplicons were cleaved with 11 restriction enzymes and fragments separated by agarose gel electrophoresis at 100 V for 45 min

<p><b>Copyright information:</b></p><p>Taken from "Identification to the species level of isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling"</p><p>BMC Microbiology 2005;5():15-15.</p><p>Published online 23 Mar 2005</p><p>PMCID:PMC1079852.</p><p>Copyright © 2005 Moreira et al; licensee BioMed Central Ltd.</p> Plus and minus signs mean positive or negative cleavage of long, medium or short PCR amplicon, respectively. GibcoBRL 1 kb DNA ladder was used for sizing DNA fragments and molecular masses are shown at right in bp