PCR amplification of rRNA spacer regions was done and the shorter amplicon was excised from an agarose gel after electrophoresis at 100 V for 45 min

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Identification to the species level of isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling"</p><p>BMC Microbiology 2005;5():15-15.</p><p>Published online 23 Mar 2005</p><p>PMCID:PMC1079852.</p><p>Copyright © 2005 Moreira et al; licensee BioMed Central Ltd.</p> Gel purified DNA was cleaved with 11 enzymes and fragments separated by electrophoresis as before. Plus and minus signs mean positive or negative cleavage of PCR amplicon. GibcoBRL 1 kb DNA ladder was used for sizing DNA fragments and molecular masses are shown at right in bp