TableS3

Chromosomal inversion data from An. arabiensi

TableS2

Chromosomal inversion data from An. coluzzi

TableS4

Chromosomal inversion data from An. funestu

Results of mixed effect logistic regression analysis on data obtained in Verano Cemetery, Rome.

<p>The model takes into account the effect of: the treatment (i.e, sentinel, SS, <i>versus</i> control, CS, sites), the replicates (i.e. Exp 1.1 and Exp 1.2) and the interaction between treatment and replicates on pyriproxyfen-related mortality. OR = odds ratio; CI = confidence interval. Statistically significant (p<0.05) odds ratios in bold.</p

Ecology and results of “auto-dissemination” experiments in an enclosed garden in Rome (Italy).

<p>a and b – views of the study area with dissemination stations and sentinel and control sites; Exp. 2.1 and Exp. 2.2: frequencies (%) of dead <i>Aedes albopictus</i> larvae/pupae (in black) and of emerged adults (in white) in the first and second experiment carried out in the study area with 5% pyriproxyfen powder; sentinel sites are numbered accordingly to <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001793#pntd-0001793-g004" target="_blank">Figure 4</a>.</p

Dissemination station (DS) used for “auto-dissemination” experiments in Rome.

<p>a – whole DS; b – higher and lower parts of DS, where arrow indicates black cotton cloth dusted with powdered pyriproxyfen; c – lower part of DS, where arrow indicates the net placed above the water level to prevent mosquitoes contacting with water.</p

<i>Aedes albopictus</i> mortality in control and sentinel sites during the experiments carried out in Rome.

<p>PPF = pyriproxyfen concentration; T = mean daily temperature during experiments; Total = percentage of dead larvae/pupae over 250 third instar larvae in 10 control sites (CS) and 10 sentinel (SS), respectively.</p

Overall mortality (%) in sentinel and control sites during the four experiments carried out in Rome.

<p>Experiment 1.1: 0,5% pyriproxyfen concentration; experiments 1.2, 2.1, 2.2: 5% pyriproxyfen concentration. Orange circles: sentinel sites numbered accordingly to <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001793#pntd-0001793-g002" target="_blank">Figures 2</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001793#pntd-0001793-g003" target="_blank">3</a>. Blue squares: control sites.</p

Caputo et al 2016 Data S1

Information on the two Sequenom iPLEX® multiplexes used to genotype the X-centromeric region of An.gambiae and An.coluzzii specimens from Guinea Bissau and Burkina Faso as described in the paper. The two iPLEX assays were designed to genotype a total of 35 X-pericentromeric species-specific SNPs, yielding a density of 1 SNP per 0.12 Mb across the 4 Mb region targeted

Caputo et al. 2016 - Supporting information 5: Sequenom design

Information on the two Sequenom iPLEX® multiplexes used to genotype the X-centromeric region of An.gambiae and An.coluzzii specimens from Guinea Bissau and Burkina Faso as described in the paper. The two iPLEX assays were designed to genotype a total of 35 X-pericentromeric species-specific SNPs, yielding a density of 1 SNP per 0.12 Mb across the 4 Mb region targeted