Benefits from remote sensing data utilization in urban planning processes and system recommendations

The benefits of utilizing remote sensor data in the urban planning process of the Metropolitan Washington Council of Governments are investigated. An evaluation of sensor requirements, a description/ comparison of costs, benefits, levels of accuracy, ease of attainment, and frequency of update possible using sensor versus traditional data acquisition techniques are discussed

Effectiveness of Strength Training Programs at Decreasing Upper Extremity Injury Rates in Youth Baseball Players: A Critically Appraised Topic

Incidence of both youth sport specialization and overuse injuries continue to rise. Baseball is a common sport where sport specialization begins in young adolescents. Youth baseball players are subject to increased risk of overuse injuries due to a combination of factors including skeletal immaturity, lack of proper biomechanics, and the heavy workloads in youth elite-level sports. Injury prevention guidelines exist, including recommendations for pitch counts and inning limits for youth baseball pitchers, as a means to reduce injury risk. However, current guidelines fail to address the need for prevention programs focusing on a variety of aspects including balance, flexibility, and strength. Focused Clinical Question: Does implementing a strength training program in youth baseball players decrease their risk of sustaining an upper extremity injury compared to players who don’t complete a strengthening program

An examination of applications of remote sensing data to Metropolitan Washington Council of Governments' planning requirements

A comprehensive inventory of a series of remote sensing applications for a variety of regional planning programs in metropolitan Washington was undertaken. Examples of application, methods for data utilization, and corresponding photographic illustrations are provided illustrating how remote sensing would prove particularly useful as a unique and/or supplemental data source

Three-dimensional structural modelling and calculation of electrostatic potentials of HLA Bw4 and Bw6 epitopes to explain the molecular basis for alloantibody binding: toward predicting HLA antigenicity and immunogenicity.

BACKGROUND: We have previously shown that qualitative assessment of surface electrostatic potential of HLA class I molecules helps explain serological patterns of alloantibody binding. We have now used a novel computational approach to quantitate differences in surface electrostatic potential of HLA B-cell epitopes and applied this to explain HLA Bw4 and Bw6 antigenicity. METHODS: Protein structure models of HLA class I alleles expressing either the Bw4 or Bw6 epitope (defined by sequence motifs at positions 77 to 83) were generated using comparative structure prediction. The electrostatic potential in 3-dimensional space encompassing the Bw4/Bw6 epitope was computed by solving the Poisson-Boltzmann equation and quantitatively compared in a pairwise, all-versus-all fashion to produce distance matrices that cluster epitopes with similar electrostatics properties. RESULTS: Quantitative comparison of surface electrostatic potential at the carboxyl terminal of the α1-helix of HLA class I alleles, corresponding to amino acid sequence motif 77 to 83, produced clustering of HLA molecules in 3 principal groups according to Bw4 or Bw6 epitope expression. Remarkably, quantitative differences in electrostatic potential reflected known patterns of serological reactivity better than Bw4/Bw6 amino acid sequence motifs. Quantitative assessment of epitope electrostatic potential allowed the impact of known amino acid substitutions (HLA-B*07:02 R79G, R82L, G83R) that are critical for antibody binding to be predicted. CONCLUSIONS: We describe a novel approach for quantitating differences in HLA B-cell epitope electrostatic potential. Proof of principle is provided that this approach enables better assessment of HLA epitope antigenicity than amino acid sequence data alone, and it may allow prediction of HLA immunogenicity.This is the author accepted manuscript. The final version is available from Wolters Kluwer via http://dx.doi.org/10.1097/TP.000000000000054

Free volume dilatation in polymers by ortho-positronium

The possibility of positronium induced free volume cavity expansion in some polymers above the glass transition temperature was investigated using experimental positron annihilation lifetime data from the literature for polydimethylsiloxane, polyisobutylene, and polybutadiene as function of temperature. The results suggest that free volume sites can expand towards an equilibrium size, determined as the equilibrium Ps-bubble size defined earlier for low-molecular-weight liquids. The expansion can be explained by the increase of molecular mobility and hence decrease of relaxation times, which at the higher temperatures approach the o-Ps lifetimes. Nanoscale viscosities were estimated using Navier-Stokes equation and were found to be several orders of magnitude lower than the macroscopic viscosity at the same temperature

Radiation measurements in a simulated non-terrestrial atmosphere

A high-speed wind tunnel has been used to experimentally simulate the flow experienced by a capsule entering a planetary atmosphere. High speed photography showed that a steady test time of approximately 50 μs existed in the facility. Holographic interferometry has been performed to measure the twodimensional density distribution around a cylinder in the flow. A peak density ratio (density normalised by the free-stream density) of about 14 was observed. Emission spectroscopy allowed the characterisation of the conditions along the stagnation streamline in front of the capsule model. The results showed a temperature that varied between 8,500 K and 11,000 K in this region

DNA methylation is associated with codon degeneracy in a species of bumblebee

Social insects display extreme phenotypic differences between sexes and castes even though the underlying genome can be almost identical. Epigenetic processes have been proposed as a possible mechanism for mediating these phenotypic differences. Using whole genome bisulfite sequencing of queens, males, and reproductive female workers we have characterised the sex- and caste-specific methylome of the bumblebee Bombus terrestris. We have identified a potential role for DNA methylation in histone modification processes which may influence sex and caste phenotypic differences. We also find differentially methylated genes generally show low levels of DNA methylation which may suggest a separate function for lowly methylated genes in mediating transcriptional plasticity, unlike highly methylated genes which are usually involved in housekeeping functions. We also examined the relationship between the underlying genome and the methylome using whole genome re-sequencing of the same queens and males. We find DNA methylation is enriched at zero-fold degenerate sites. We suggest DNA methylation may be acting as a targeted mutagen at these sites, providing substrate for selection via non-synonymous changes in the underlying genome. However, we did not see any relationship between DNA methylation and rates of positive selection in our samples. In order to fully assess a possible role for DNA methylation in adaptive processes a specifically designed study using natural population data is needed.</p