Coronavirus Disease 2019: Prevention and Safety in the Dental Office

Coronavirus disease 2019 (COVID-19) caused by a single stranded RNA virus originating from Wuhan China has gripped the whole world and evolved rapidly into a public health crisis. SARSCoV-2 (severe acute respiratory syndrome coronavirus 2 is abundantly present in nasopharyngeal and salivary secretions of affected patients and is transmitted by droplets, surface contact, fecal-oral route and by aerosolization during procedures. The dental professionals are particularly at risk because of close contact with the patient and exposure to blood, saliva and droplets. Dental professionals must be fully aware of coronavirus spreading modalities, identification of patients with this infection, so present article introduces the essential knowledge about COVID-19 infection and provide recommended management protocols for dental practitioners based on relevant guidelines, research so that dental professional are better prepared to manage  asymptomatic, suspected, and confirmed COVID-19 patients

The Role of <em>Pseudomonas aeruginosa</em> DsbA-1 in Bacterial Pathogenesis: Current Research and Future Prospects

Disulfide bond isomerase proteins (Dsbs) have been extensively characterized in gram-negative bacteria. Recently research efforts is being placed on their biology in gram-positive species. Modern “omics” technologies, allowed assessment of the contribution of the Dsbs to bacterial pathogenesis. The author cloned and characterized the DsbA 1 protein from Pseudomonas aeruginosa in the late 1990s. The global proteome analysis demonstrated that the dsbA gene is under the direct regulatory control of the extracytoplasmic function (ECF) sigma factor AlgT(U) or sigma-22. This is unique to P. aeruginosa. Disruption of dsbA gene results in pleiotropic phenotype: defect in assembly of cysteine disulfide bond containing proteins-as shown in many others. Recently, omics-based approaches identified expression changes in dsbA gene under different physiological states of bacterial pathogens-primarily in free-living, biofilm state, or under infectious disease conditions. Involvement of DsbA function in biofilm formation was shown using dsbA gene disruption mutants. This chapter documents past and current findings and concludes with future trends in research on Dsbs including peptidomimetics

Clinico-Haematological Profile of Acute Megakaryoblastic Leukaemia: Report of Five Cases

Acute megakaryoblastic leukemia (AMKL) is a rare subtype of acute myeloid leukemia. Although known as a distinct entity for a very long time, because of lack of distinct clinical features and morphological criteria, it is difficult to diagnose this variant correctly. We herein present the clinical, morphological, cytochemical, and immunocytochemical features of five cases of AMKL. Certain morphological features such as presence of abnormal platelet count, giant platelets, and cytoplasmic blebbing in blasts were found to be important pointers towards the diagnosis. However, none of the features were found to be consistent and thus morphological diagnosis has to be confirmed by cytochemistry and immunocytochemistry

Myco-Biocontrol of Insect Pests: Factors Involved, Mechanism, and Regulation

The growing demand for reducing chemical inputs in agriculture and increased resistance to insecticides have provided great impetus to the development of alternative forms of insect-pest control. Myco-biocontrol offers an attractive alternative to the use of chemical pesticides. Myco-biocontrol agents are naturally occurring organisms which are perceived as less damaging to the environment. Their mode of action appears little complex which makes it highly unlikely that resistance could be developed to a biopesticide. Past research has shown some promise of the use of fungi as a selective pesticide. The current paper updates us about the recent progress in the field of myco-biocontrol of insect pests and their possible mechanism of action to further enhance our understanding about the biological control of insect pests

Inflammatory myofibroblastic tumor of inguinal lymph nodes, simulating lymphoma

Multiple enlarged lymph nodes in an elderly female patient can have varied etiologies as well as histologic pictures. We are presenting the case of a 53-year-old female who presented with inguinal lymphadenopathy with fever, which was clinically misconstrued as lymphoma. Cytology could not exclude a lymphoma. Histology led to the unusual diagnosis of inflammatory myofibroblastic tumor of lymph node in this case. Inflammatory myofibroblastic tumor of the lymph node is a rare, distinctive reactive proliferative pattern in the lymph node which involves proliferation of the connective tissue elements of the lymph node, admixed with lymphocytes, plasma cells, eosinophils, and histiocytes. Multiple etiologic agents have been suggested in existing literature. Despite extensive search, no definite attributable cause could be sought. It is now widely accepted that inflammatory pseudotumor of the lymph node is a non-neoplastic proliferation which has a benign clinical course and excellent prognosis after surgical resection

Dr Preeti Malhotra: Assistant professor, Department of Obstetrics and Gynaecology, Pacific Institute of Medical sciences, Udaipur.

Objective- Knowing the ideal levels of T3, T4, FT3, FT4 and TSH for each pregnancy trimester is the goal. Methodology-The Department of Obstetrics and Gynecology at the Pacific Institute of Medical Sciences in Udaipur treated 150 patients (obstetric cases) for the current longitudinal research. The following parameters were examined for in a fasting blood sample: T3 (Triiodothyronine) (Triiodothyronine). T4 (Thyroxine), FT3, FT4 and TSH, (Thyrotropin Stimulating Hormone). Results- As a result, thyroid profile readings in every group (I, II and III trimester) were increased and were maximum in second group or second trimester followed by third. The levels above the normal range were in 39 (12+12+15) women of all the groups. In conclusion, the second trimester of pregnancy saw the greatest increase in thyroid hormones readings. Thyroid profile was lower during the first trimester, particularly at par during the second, and decreased in third trimester.&nbsp

Proteome Analysis of the Effect of Mucoid Conversion on . . .

hesis were expected among these, and two (AlgA and AlgD) were identified. This result verified that the 2-D gel approach could identify gene products under sigma-22 control and upregulated by mucA mutation. Two other protein spots were also clearly upregulated in the mucA22 background, and these were identified as porin F (an outer membrane protein) and a homologue of DsbA (a disulfide bond isomerase). Single-copy gene fusions were constructed to test whether these proteins were enhanced in the mucoid strain due to increased transcription. The oprF-lacZ fusion showed little difference in levels of expression in the two strains. However, the dsbA-lacZ fusion showed two- to threefold higher expression in PDO300 than in PAO1, suggesting that its promoter was upregulated by the deregulation of sigma-22 activity. A dsbA-null mutant was constructed in PAO1 and shown to have defects predicted for a cell with reduced disulfide bond isomerase activity, namely, reduction in periplasmi