Sorting Strategies for Long Yearling Cattle Grown in an Extensive Forage Utilization Beef Production System

One hundred sixty English-cross steers (244 kg, SD=23 kg) were used in each yr of a 2-yr study to determine effects of sorting on performance, carcass characteristics, variability, and profitability in a long yearling system utilizing ranch-source calves. Steers were back grounded during winter then grazed smooth brome-grass pastures followed by warm season native range prior to entering the feedlot in the fall. Steers were stratified by BW and allotted to 1 of 4 treatments: 1) 40 head sorted by pre-grazing BW where heavy steers entered the feedlot in July (PST), 2) 40 head sorted by BW entering the feedlot (FDL), 3) 60 head sorted by BW and fat thickness at the end of the feeding period (IND), and 4) 20 head that were not sorted (UNS). No differences were observed for performance during back-grounding or grazing phases (P \u3e 0.30). Cattle receiving PST sorting weighed less entering the feedlot and consumed less feed with less feedlot ADG compared to other treatments (P \u3c 0.05). No differences in feed efficiencies were detected (P = 0.84). Cattle receiving PST sorting had greater marbling scores (P \u3c 0.05) and less variation in BW upon feedlot entry which resulted in less variation in carcass weight compared with UNS (P \u3c 0.05). Cattle sorted by BW entering the feedlot tended (P = 0.08) to have less variation in carcass weight compared with UNS. There were no differences in carcass weight or profitability when calculated on either a live or value basis. No sorting strategy increased carcass weight, reduced discounts for overweight or corpulent carcasses, or improved profitability over UNS

Sorting Strategies for Long Yearling Cattle Grown in an Extensive Forage Utilization Beef Production System

One hundred sixty English-cross steers (244 kg, SD=23 kg) were used in each yr of a 2-yr study to determine effects of sorting on performance, carcass characteristics, variability, and profitability in a long yearling system utilizing ranch-source calves. Steers were back grounded during winter then grazed smooth brome-grass pastures followed by warm season native range prior to entering the feedlot in the fall. Steers were stratified by BW and allotted to 1 of 4 treatments: 1) 40 head sorted by pre-grazing BW where heavy steers entered the feedlot in July (PST), 2) 40 head sorted by BW entering the feedlot (FDL), 3) 60 head sorted by BW and fat thickness at the end of the feeding period (IND), and 4) 20 head that were not sorted (UNS). No differences were observed for performance during back-grounding or grazing phases (P \u3e 0.30). Cattle receiving PST sorting weighed less entering the feedlot and consumed less feed with less feedlot ADG compared to other treatments (P \u3c 0.05). No differences in feed efficiencies were detected (P = 0.84). Cattle receiving PST sorting had greater marbling scores (P \u3c 0.05) and less variation in BW upon feedlot entry which resulted in less variation in carcass weight compared with UNS (P \u3c 0.05). Cattle sorted by BW entering the feedlot tended (P = 0.08) to have less variation in carcass weight compared with UNS. There were no differences in carcass weight or profitability when calculated on either a live or value basis. No sorting strategy increased carcass weight, reduced discounts for overweight or corpulent carcasses, or improved profitability over UNS

Compact, Intelligent, Digitally Controlled IGBT Gate Drivers for a PEBB-Based ILC Marx Modulator

SLAC National Accelerator Laboratory has built and is currently operating a first generation prototype Marx klystron modulator to meet ILC specifications. Under development is a second generation prototype, aimed at improving overall performance, serviceability, and manufacturability as compared to its predecessor. It is designed around 32 cells, each operating at 3.75 kV and correcting for its own capacitor droop. Due to the uniqueness of this application, high voltage gate drivers needed to be developed for the main 6.5 kV and droop correction 1.7 kV IGBTs. The gate driver provides vital functions such as protection of the IGBT from over-voltage and over-current, detection of gate-emitter open and short circuit conditions, and monitoring of IGBT degradation (based on collector-emitter saturation voltage). Gate drive control, diagnostic processing capabilities, and communication are digitally implemented using an FPGA. This paper details the design of the gate driver circuitry, component selection, and construction layout. In addition, experimental results are included to illustrate the effectiveness of the protection circuit

Design Considerations for a PEBB-Based Marx-Topology ILC Klystron Modulator

The concept of Power Electronic Building Blocks (PEBBs) has its origin in the U.S. Navy during the last decade of the past century. As compared to a more conventional or classical design approach, a PEBB-oriented design approach combines various potential advantages such as increased modularity, high availability and simplified serviceability. This relatively new design paradigm for power conversion has progressively matured since then and its underlying philosophy has been clearly and successfully demonstrated in a number of real-world applications. Therefore, this approach has been adopted here to design a Marx-topology modulator for an International Linear Collider (ILC) environment where easy serviceability and high availability are crucial. This paper describes various aspects relating to the design of a 32-cell Marx-topology ILC klystron modulator. The concept of nested droop correction is introduced and illustrated. Several design considerations including cosmic ray withstand, power cycling capability, fault tolerance, etc., are discussed. Details of the design of a Marx cell PEBB are included

Enhanced mitochondrial genome analysis: bioinformatic and long-read sequencing advances and their diagnostic implications

Introduction: Primary mitochondrial diseases (PMDs) comprise a large and heterogeneous group of genetic diseases that result from pathogenic variants in either nuclear DNA (nDNA) or mitochondrial DNA (mtDNA). Widespread adoption of next-generation sequencing (NGS) has improved the efficiency and accuracy of mtDNA diagnoses; however, several challenges remain. Areas covered: In this review, we briefly summarize the current state of the art in molecular diagnostics for mtDNA and consider the implications of improved whole genome sequencing (WGS), bioinformatic techniques, and the adoption of long-read sequencing, for PMD diagnostics. Expert opinion: We anticipate that the application of PCR-free WGS from blood DNA will increase in diagnostic laboratories, while for adults with myopathic presentations, WGS from muscle DNA may become more widespread. Improved bioinformatic strategies will enhance WGS data interrogation, with more accurate delineation of mtDNA and NUMTs (nuclear mitochondrial DNA segments) in WGS data, superior coverage uniformity, indirect measurement of mtDNA copy number, and more accurate interpretation of heteroplasmic large-scale rearrangements (LSRs). Separately, the adoption of diagnostic long-read sequencing could offer greater resolution of complex LSRs and the opportunity to phase heteroplasmic variants

Mitochondrial DNA analysis from exome sequencing data improves the diagnostic yield in neurological diseases

A rapidly expanding catalogue of neurogenetic disorders has encouraged a diagnostic shift towards early clinical whole exome sequencing (WES). Adult primary mitochondrial diseases (PMDs) frequently exhibit neurological manifestations that overlap with other nervous system disorders. However, mitochondrial DNA (mtDNA) is not routinely analyzed in standard clinical WES bioinformatic pipelines. We reanalyzed 11,424 exomes, enriched with neurological diseases, for pathogenic mtDNA variants. Twenty‐four different mtDNA mutations were detected in 64 exomes, 11 of which were considered disease causing based on the associated clinical phenotypes. These findings highlight the diagnostic uplifts gained by analyzing mtDNA from WES data in neurological diseases

Boundaries of Semantic Distraction: Dominance and Lexicality Act at Retrieval

Three experiments investigated memory for semantic information with the goal of determining boundary conditions for the manifestation of semantic auditory distraction. Irrelevant speech disrupted the free recall of semantic category-exemplars to an equal degree regardless of whether the speech coincided with presentation or test phases of the task (Experiment 1) and occurred regardless of whether it comprised random words or coherent sentences (Experiment 2). The effects of background speech were greater when the irrelevant speech was semantically related to the to-be-remembered material, but only when the irrelevant words were high in output dominance (Experiment 3). The implications of these findings in relation to the processing of task material and the processing of background speech is discussed

Working memory capacity modulates habituation rate: Evidence from a cross-modal auditory distraction paradigm

Habituation of the orienting response is a pivotal part of selective attention, and previous research has related working memory capacity (WMC) to attention control. Against this background, the purpose of this study was to investigate whether individual differences in WMC contribute to habituation rate. The participants categorized visual targets across six blocks of trials. Each target was preceded either by a standard sound or, on rare trials, by a deviant. The magnitude of the deviation effect (i.e., prolonged response time when the deviant was presented) was relatively large in the beginning but attenuated toward the end. There was no relationship between WMC and the deviation effect at the beginning, but there was at the end, and greater WMC was associated with greater habituation. These results indicate that high memory ability increases habituation rate, and they support theories proposing a role for cognitive control in habituation and in some forms of auditory distraction

Seasonality of Influenza A(H3N2) Virus: A Hong Kong Perspective (1997–2006)

BACKGROUND: The underlying basis for the seasonality of influenza A viruses is still uncertain. Phylogenetic studies investigated this phenomenon but have lacked sequences from more subtropical and tropical regions, particularly from Southeast Asia. METHODOLOGY/PRINCIPAL FINDINGS: 281 complete hemagglutinin (HA) and neuraminidase (NA) sequences were obtained from influenza A(H3N2) viruses, collected over 10 years (1997-2006) from Hong Kong. These dated sequences were analyzed with influenza A(H3N2) vaccine strain sequences (Syd/5/97, Mos/10/99, Fuj/411/02, Cal/7/04) and 315 other publicly available dated sequences from elsewhere, worldwide. In addition, the NA sequence alignment was inspected for the presence of any naturally occurring, known, neuraminidase inhibitor (NAI) resistance-associated amino acid mutations (R292K and E119V). Before 2001, the Hong Kong HA and NA sequences clustered more closely with the older vaccine sequences (Syd/5/97, Mos/10/99) than did sequences from elsewhere. After 2001, this trend reversed with significant clusters containing HA and NA sequences from different locations, isolated at different times, suggesting that viral migration may account for much of the influenza A(H3N2) seasonality during this 10-year period. However, at least one example from Hong Kong was found suggesting that in some years, influenza A(H3N2) viruses may persist in the same location, perhaps continuing to circulate, sub-clinically, at low levels between seasons, to re-emerge in the influenza season the following year, relatively unchanged. None of these Hong Kong influenza A(H3N2) NA sequences contained any of the known NAI-resistance associated mutations. CONCLUSIONS/SIGNIFICANCE: The seasonality of influenza A(H3N2) may be largely due to global migration, with similar viruses appearing in different countries at different times. However, occasionally, some viruses may remain within a single location and continue to circulate within that population, to re-emerge during the next influenza season, with relatively little genetic change. Naturally occurring NAI resistance mutations were absent or, at least, very rare in this population