259 research outputs found
Perceptual Evaluation of Synthesized Sound Effects
Sound synthesis is the process of generating artificial sounds through some form of simulation or modelling. This article aims to identify which sound synthesis methods achieve the goal of producing a believable audio sample that may replace a recorded sound sample. A perceptual evaluation experiment of five different sound synthesis techniques was undertaken. Additive synthesis, statistical modelling synthesis with two different feature sets, physically inspired synthesis, concatenative synthesis, and sinusoidal modelling synthesis were all compared. Evaluation using eight different sound class stimuli and 66 different samples was undertaken. The additive synthesizer is the only synthesis method not considered significantly different from the reference sample across all sounds classes. The results demonstrate that sound synthesis can be considered as realistic as a recorded sample and makes recommendations for use of synthesis methods, given different sound class contexts
Leaf yellowing of the wheat cultivar Mace in the absence of yellowspot disease
The wheat variety Mace is currently dominating the southern wheat growing regions of Australia. It is high yielding in most environments and resistant to many diseases including yellow spot (also known as tan spot). However, observations of foliar yellowing of Mace have recently been reported in the field. This has raised concerns over a possible breakdown of resistance to yellow spot, which is caused by the necrotrophic fungal pathogen Pyrenophora triticirepentis. West Australian field samples of yellowing Mace leaves were evaluated for P. triticirepentis infection, and this pathogen was determined to be absent. Instead, Alternaria spp. were isolated from the wheat leaves. Pathogenicity assays showed that the recovered Alternaria spp. were unable to cause disease symptoms on Mace. Furthermore, spontaneous foliar lesions were observed in Mace grown in the absence of pathogens. It is therefore likely that such yellowing is a physiological trait, which will not respond to fungicide application. A marginal impact on yield cannot be excluded
The Hawking-Page crossover in noncommutative anti-deSitter space
We study the problem of a Schwarzschild-anti-deSitter black hole in a
noncommutative geometry framework, thought to be an effective description of
quantum-gravitational spacetime. As a first step we derive the noncommutative
geometry inspired Schwarzschild-anti-deSitter solution. After studying the
horizon structure, we find that the curvature singularity is smeared out by the
noncommutative fluctuations. On the thermodynamics side, we show that the black
hole temperature, instead of a divergent behavior at small scales, admits a
maximum value. This fact implies an extension of the Hawking-Page transition
into a van der Waals-like phase diagram, with a critical point at a critical
cosmological constant size in Plank units and a smooth crossover thereafter. We
speculate that, in the gauge-string dictionary, this corresponds to the
confinement "critical point" in number of colors at finite number of flavors, a
highly non-trivial parameter that can be determined through lattice
simulations.Comment: 24 pages, 6 figure, 1 table, version matching that published on JHE
Running Gauge Coupling in Asymptotically Safe Quantum Gravity
We investigate the non-perturbative renormalization group behavior of the
gauge coupling constant using a truncated form of the functional flow equation
for the effective average action of the Yang-Mills-gravity system. We find a
non-zero quantum gravity correction to the standard Yang-Mills beta function
which has the same sign as the gauge boson contribution. Our results fit into
the picture according to which Quantum Einstein Gravity (QEG) is asymptotically
safe, with a vanishing gauge coupling constant at the non-trivial fixed point.Comment: 27 page
Monoubiquitination of syntaxin 3 leads to retrieval from the basolateral plasma membrane and facilitates cargo recruitment to exosomes
Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in these organelles is unknown. Here we report that Stx3 undergoes monoubiquitination in a conserved polybasic domain. Stx3 present at the basolateral—but not the apical—plasma membrane is rapidly endocytosed, targeted to endosomes, internalized into intraluminal vesicles (ILVs), and excreted in exosomes. A nonubiquitinatable mutant of Stx3 (Stx3-5R) fails to enter this pathway and leads to the inability of the apical exosomal cargo protein GPRC5B to enter the ILV/exosomal pathway. This suggests that ubiquitination of Stx3 leads to removal from the basolateral membrane to achieve apical polarity, that Stx3 plays a role in the recruitment of cargo to exosomes, and that the Stx3-5R mutant acts as a dominant-negative inhibitor. Human cytomegalovirus (HCMV) acquires its membrane in an intracellular compartment and we show that Stx3-5R strongly reduces the number of excreted infectious viral particles. Altogether these results suggest that Stx3 functions in the transport of specific proteins to apical exosomes and that HCMV exploits this pathway for virion excretion
siRNA Screening of a Targeted Library of DNA Repair Factors in HIV Infection Reveals a Role for Base Excision Repair in HIV Integration
Host DNA repair enzymes have long been assumed to play a role in HIV replication,
and many different DNA repair factors have been associated with HIV. In order to
identify DNA repair pathways required for HIV infection, we conducted a targeted
siRNA screen using 232 siRNA pools for genes associated with DNA repair. Mapping
the genes targeted by effective siRNA pools to well-defined DNA repair pathways
revealed that many of the siRNAs targeting enzymes associated with the short
patch base excision repair (BER) pathway reduced HIV infection. For six siRNA
pools targeting BER enzymes, the negative effect of mRNA knockdown was rescued
by expression of the corresponding cDNA, validating the importance of the gene
in HIV replication. Additionally, mouse embryo fibroblasts (MEFs) lacking
expression of specific BER enzymes had decreased transduction by HIV-based
retroviral vectors. Examining the role BER enzymes play in HIV infection
suggests a role for the BER pathway in HIV integration
The Pioneer Anomaly
Radio-metric Doppler tracking data received from the Pioneer 10 and 11
spacecraft from heliocentric distances of 20-70 AU has consistently indicated
the presence of a small, anomalous, blue-shifted frequency drift uniformly
changing with a rate of ~6 x 10^{-9} Hz/s. Ultimately, the drift was
interpreted as a constant sunward deceleration of each particular spacecraft at
the level of a_P = (8.74 +/- 1.33) x 10^{-10} m/s^2. This apparent violation of
the Newton's gravitational inverse-square law has become known as the Pioneer
anomaly; the nature of this anomaly remains unexplained. In this review, we
summarize the current knowledge of the physical properties of the anomaly and
the conditions that led to its detection and characterization. We review
various mechanisms proposed to explain the anomaly and discuss the current
state of efforts to determine its nature. A comprehensive new investigation of
the anomalous behavior of the two Pioneers has begun recently. The new efforts
rely on the much-extended set of radio-metric Doppler data for both spacecraft
in conjunction with the newly available complete record of their telemetry
files and a large archive of original project documentation. As the new study
is yet to report its findings, this review provides the necessary background
for the new results to appear in the near future. In particular, we provide a
significant amount of information on the design, operations and behavior of the
two Pioneers during their entire missions, including descriptions of various
data formats and techniques used for their navigation and radio-science data
analysis. As most of this information was recovered relatively recently, it was
not used in the previous studies of the Pioneer anomaly, but it is critical for
the new investigation.Comment: 165 pages, 40 figures, 16 tables; accepted for publication in Living
Reviews in Relativit
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