16 research outputs found
Constructing ethanol-derived bioactive extracts using the brown seaweed Zonaria tournefortii (J.V.Lamouroux) Montagne performed with Timatic extractor by means of response surface methodology (RSM)
Understanding the biochemical and antioxidant composition and capacity of a target biomass is the first step to its
selectivity as functional food which can enhance the ability to promote health by reducing the risk of chronic diseases.
The main purpose of this work was to employ response surface methodology (RSM) to determine the effect of the
independent variables, % of ethanol (50â96%), time of sonication (0â20 min), and number of extraction cycles (6â18
cycles) in the primary extract of brown seaweed Zonaria tournefortii, studying the yield variation of some bioactive
compounds, assessing the potential of these bioextracts to integrate as a natural additives or supplements in the functional
food industry. The extractions were performed employing âGreen Chemistryâ techniques executed with the Timatic
extractor, which applied pressurized ethanol solution at a maximum pressure of 8.5 bar through milled dehydrated biomass
in the extraction vessel. Several parameters were assessed in the primary bioactive extract which included extract yield
(11.56â28.49 g (100 g)â1 dw), total chlorophyll content (0.14â1.42 g (100 g)â1 dw), total carotenoid content (0.35â0.80 g
(100 g)â1 dw), total fucoxanthin content (0.04â0.13 g (100 g)â1 dw), total phenolic content (3.58â5.84 g (100 g)â1 dw),
total flavonoid content (0.22â4.70 g (100 g)â1 dw), DPPH (56.05â76.45%), and reducing activity (3.83â6.04 g (100 g)â1
dw). A second objective was to determine the suitability of the residue for subsequent extraction of valuable compounds
such as fucoidan (4.87 to 6.59 g (100 g)â1 dw) and cellulose (18.88 to 20.27 g (100 g)â1 dw), implementing the first step to
a biorefinery strategy, using a cascade approach.info:eu-repo/semantics/publishedVersio
Validation of a spectrophotometric methodology for a rapid iodine analysis in algae and seaweed casts
Iodine plays an important role in human metabolism and its deficiency is particularly harmful in pregnancy and childhood. It remains a major public health concern
in many countries, especially in Portugal. The main purpose of this work was to develop a validated spectrophotometric analysis for a fast and reliable iodine
quantification in algal samples. Absorbance was determined at 410 nm demonstrating a good linearity (R2 â 1.0) in the range of 0â0.06 mg I/100 g. LOD and LOQ
were 1.7 Ă 10â3 and 5.0 Ă 10â3 mg I/100 g, respectively. Accuracy was determined using recovery and varied between 101 and 118%. For precision analysis, an
intra-day test performance (RSD = 8.7%) and a repeatability assay (RSD = 3.8%) were performed. Matrix effect assessment demonstrated that this had a negligible
effect (3.2%) in the iodine quantification. The spectrophotometric method was externally validated, for iodine quantification in algal samples, by INSA certified
laboratory. The correlation coefficient between external iodine quantification and our work was R2 â 0.9, showing a good correlation. Applicability was assessed in
25 macroalgae species (5 green, 9 red and 11 brown), 12 seaweed casts, collected in Canary Islands and 1 microalga (Isochrysis galbana) provided by ITC (Instituto
Tecnologico de Canarias).This work was financially supported by ISOPlexis, University of
Madeira; DemoBlueAlgae âDesenvolvimento de metodologias e
optimização dos processos de cultivo e processamento de macroalgas
para a indĂșstria e economia azulâ PROCiĂȘncia 2020 (M1420-01-0247-
FEDER000002); MACBIOBLUE âProyecto demostrativo y de transferencia tecnolĂłgica para ayudar a las empresas a desarrollar nuevos
produtos y procesos en el ambito de la BiotecnologĂa Azul de la
Macaronesiaâ (MAC/1.1b/086), program Interreg MAC 2014â2020;
ARDITI - Regional Agency for the Development of Research Technology
and Innovation (M14-20-09-5369-FSE-000001) Doctorate in Business
and UBQ II, Unidade de BioquĂmica (UBQ II company). Also, the authors present their acknowledgment to DRCT (Azores Regional
Government) for co-financing, the Portuguese National Funds, through
FCT-Fundação para a CiĂȘncia e a Tecnologia, and as applicable co-financed by the FEDER within the PT2020 Partnership Agreement by
funding the cE3c centre (UID/BIA/00329/2013) and to DRCT for
funding Azorean Biodiversity Group.info:eu-repo/semantics/publishedVersio
Helminth parasites of the oceanic horse mackerel Trachurus picturatus Bowdich 1825 (Pisces: Carangidae) from Madeira Island, Atlantic Ocean, Portugal
The helminth parasite fauna of the oceanic horse mackerel Trachurus picturatus Bowdich 1825, caught off the Madeira Islands was composed of six different taxa. Prevalence and abundance of larval Anisakis sp. (Nematoda: Anisakidae) and Nybelinia lingualis (Trypanorhyncha: Tentaculariidae), the most common parasite taxa, were 24.3%, 0.9 and 37.9%, 0.7, respectively. Bolbosoma vasculosum (Acanthocephala: Polymorphidae) and the monogeneans Heteraxinoides atlanticus (Monogenea: Heteraxinidae) and Pseudaxine trachuri (Monogenea: Gastrocotylidae) were comparatively rare. The depauperate helminth fauna of the oceanic horse mackerel at Madeira compared to other geographical regions of the north-eastern Atlantic, namely the Azores banks and the West African coast, may be attributed to the paucity of nutrients off oceanic islands and to a low density of the fish population.This work was partially supported by the Portuguese
Foundation for Science and Technology (FCT) Pluriannual
Programs.info:eu-repo/semantics/publishedVersio
Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detection
Fucoxanthin is considered an important marine bioactive compound with biological properties with promising effects, namely on health. A simple and efficient
analytical methodology is proposed for its quantification in seaweed biomass by using vortex-assisted solid-liquid microextraction (VASLME) followed by reversed
phase high-performance liquid chromatography (RP-HPLC) photodiode array detection (PDA) analysis. This microextraction uses reduced quantities of sample
(25 mg) and solvent (300 ÎŒL of ethanol) to efficiently extract this high-valued xanthophyll, in a vortex time of 15 min. These extraction parameters were optimized
performing a Central Composite Design (CCD) analysis, running 32 individual experiments. In turn, the method validation was assessed. The linearity of the method
was confirmed (R2 = 0.99998) in a concentration range from 12 to 3600 ÎŒg·gâ1 dw. Also, good sensitivity and accuracy results were observed through the LOD
(3.33 ÎŒg·gâ1
), LOQ (10.09 ÎŒg·gâ1
) and recovery (varied from 95 to 97%) assessments. Good precision was also verified, with intra-day variation within 2.0â3.3%,
and inter-day within 1.0â3.8%. Matrix effect was also evaluated and an acceptable variation of 3.4% was found. The method applicability was confirmed by the
analysis of 22 seaweed biomass samples and fucoxanthin content was found to vary from about 10 to 853 ÎŒg·gâ1 dw. This method demonstrated a good performance
and can be successfully implemented for a rapid, reliable and accurate screening of fucoxanthin in seaweed biomass.info:eu-repo/semantics/publishedVersio
Nutraceutical potential of Asparagopsis taxiformis (Delile) Trevisan extracts and assessment of a downstream purification strategy
The main goal of the present work was to determine the nutraceutical potential of Asparagopsis taxiformis D. extracts from Madeira Archipelago south coast. Extraction methodologies consisted either/or in 72 hours stirring, at room temperature (M1), or 6 cycles of Soxhlet extraction (M2), both with re-extraction. Solvents used were distilled water, ethanol, methanol and ethyl acetate. M1 allowed to obtain the highest values for extraction yield (31.65 g.100gâ1 dw) using water, whereas iodine content (3.37 g.100gâ1 dw), TPC (1.71 g GAE.100gâ1 dw) and chlorophyll a (45.96 mg.100gâ1 dw) were obtained using ethanol, and TCC (36.23 mg.100gâ1 dw) with methanol. Extracts that showed higher reduction activity in M1 were derived from ethanol extraction (1,908 mg AAE.100gâ1 dw). Water and ethanol were the best solvents for higher DPPH scavenging activity in M2, both with same result (IC50 1.37 mg.mLâ1). The lowest value of IC50 for chelating activity (1.57 mg.mLâ1) was determined in M1, using ethyl acetate. The remaining residue was used to obtain other products, i.e. lipid extraction (M1, 2.05 g.100gâ1 dw), carrageenans (M2, 21.18 g.100gâ1 dw) and cellulose (M1, 23.81 g.100gâ1 dw) with subsequent FTIR ATR analysis. Our results show that A. taxiformis is a valuable source of bioactive compounds. The M1 extraction methodology using ethanol is the most effective solvent to produce an iodine rich bioactive extract with potential of being used as a nutraceutical supplement. Also, we have demonstrated a possible downstream strategy that could be implemented for multiple compound extraction from A. taxiformis residue. This has a vital importance for future feasibility, when using this biomass as an industrial feedstock for multiple products production. Statistical analysis, using SPSS 24.0, was also performed and important correlations were found between assays and methods