Photo sensor array technology development

The development of an improved capability photo sensor array imager for use in a Viking '75 type facsimile camera is presented. This imager consists of silicon photodiodes and lead sulfide detectors to cover a spectral range from 0.4 to 2.7 microns. An optical design specifying filter configurations and convergence angles is described. Three electronics design approaches: AC-chopped light, DC-dual detector, and DC-single detector, are investigated. Experimental and calculated results are compared whenever possible using breadboard testing and tolerance analysis techniques. Results show that any design used must be forgiving of the relative instability of lead sulfide detectors. A final design using lead sulfide detectors and associated electronics is implemented by fabrication of a hybrid prototype device. Test results of this device show a good agreement with calculated values

PRELIMINARY RESULTS OF THE SNAP 2 EXPERIMENTAL REACTOR

The operating history of the SNAP 2 Experimental Reactor (SER) and the preliminary results from the testing program are presented. The total energy generated during the life of the reactor was 224,650 kilowatt hours. This is equivalent to approximately one-half year of full-power operation. The methods used to obtain the reactor parameters are also described. The experimental data obtained were generally in excellent agreement with calculated valuess The principal comparisons are tabulated. (auth

Neutrinos from Early-Phase, Pulsar-Driven Supernovae

Neutron stars, just after their formation, are surrounded by expanding, dense, and very hot envelopes which radiate thermal photons. Iron nuclei can be accelerated in the wind zones of such energetic pulsars to very high energies. These nuclei photo-disintegrate and their products lose energy efficiently in collisions with thermal photons and with the matter of the envelope, mainly via pion production. When the temperature of the radiation inside the envelope of the supernova drops below $\sim 3\times 10^6$ K, these pions decay before losing energy and produce high energy neutrinos. We estimate the flux of muon neutrinos emitted during such an early phase of the pulsar - supernova envelope interaction. We find that a 1 km$^2$ neutrino detector should be able to detect neutrinos above 1 TeV within about one year after the explosion from a supernova in our Galaxy. This result holds if these pulsars are able to efficiently accelerate nuclei to energies $\sim 10^{20}$ eV, as postulated recently by some authors for models of Galactic acceleration of the extremely high energy cosmic rays (EHE CRs).Comment: 16 pages, 3 figures, revised version submitted to Ap

The Actin Binding Protein, Fesselin, is a Member of the Synaptopodin Family

Fesselin is a natively unfolded protein that is abundant in avian smooth muscle. Like many natively unfolded proteins, fesselin has multiple binding partners including actin, myosin, calmodulin and α-actinin. Fesselin accelerates actin polymerization and bundles actin. These and other observations suggest that fesselin is a component of the cytoskeleton. We have now cloned fesselin and have determined the cDNA derived amino acid sequence. We verified parts of the sequence by Edman analysis and by mass spectroscopy. Our results confirmed fesselin is homologous to human synaptopodin 2 and belongs to the synaptopodin family of proteins. Originally published Biochem Biophys Res Commun. Vol. 371, No. 3, July 200

Applications of aerospace technology in biology and medicine

Utilization of National Aeronautics and Space Administration (NASA) technology in medicine is discussed. The objective is best obtained by stimulation of the introduction of new or improved commercially available medical products incorporating aerospace technology. A bipolar donor/recipient model of medical technology transfer is presented to provide a basis for the team's methodology. That methodology is designed to: (1) identify medical problems and NASA technology that, in combination, constitute opportunities for successful medical products; (2) obtain the early participation of industry in the transfer process; and (3) obtain acceptance by the medical community of new medical products based on NASA technology. Two commercial transfers were completed: the Stowaway, a lightweight wheelchair that provides mobility for the disabled and elderly in the cabin of commercial aircraft, and Micromed, a portable medication infusion pump for the reliable, continuous infusion of medications such as heparin or insulin. The marketing and manufacturing factors critical to the commercialization of the lightweight walker incorporating composite materials were studied. Progress was made in the development and commercialization of each of the 18 currently active projects

Characterization of a polymorphism in NAD(P)H: quinone oxidoreductase (DT-diaphorase).

NAD(P)H:quinone oxidoreductase (NQO1, EC 1.6.99.2) is an obligate two-electron reductase that can either bioactivate or detoxify quinones and has been proposed to play an important role in chemoprevention. We have previously characterized a homozygous point mutation in the BE human colon carcinoma cell line that leads to a loss of NQO1 activity. Sequence analysis showed that this mutation was at position 609 of the NQO1 cDNA, conferring a proline to serine substitution at position 187 of the NQO1 enzyme. Using polymerase chain reaction (PCR) analysis, we have found that the H596 human non-small-cell lung cancer (NSCLC) cell line has elevated NQO1 mRNA, but no detectable enzyme activity. Sequencing of the coding region of NQO1 from the H596 cells showed the presence of the identical homozygous point mutation present in the BE cell line. Expression and purification of recombinant wild-type and mutant protein from E. coli showed that mutant protein could be detected using immunoblot analysis and had 2% of the enzymatic activity of the wild-type protein. PCR and Northern blot analysis showed moderate to low levels of expression of the correctly sized transcript in the mutant cells. Immunoblot analysis also revealed that recombinant mutant protein was immunoreactive; however, the mutant protein was not detected in the cytosol of either BE or H596 cells, suggesting that the mutant proteins were either not translated or were rapidly degraded. The absence of any detectable, active protein, therefore, appears to be responsible for the lack of NQO1 activity in cells homozygous for the mutation. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for the mutation at position 609 conducted on 90 human lung tissue samples (45 matched sets of tumour and uninvolved tissue) revealed a 7% incidence of individuals homozygous for the mutation, and 42% heterozygous for the mutation. These data suggest that the mutation at position 609 represents a polymorphism in an important xenobiotic metabolizing enzyme, which has implications for cancer therapy, chemoprevention and chemoprotection

On the Hadronic Beam Model for Gamma-ray Production in Blazars

We consider, herein, a model for gamma-ray production in blazars in which a relativistic, highly-collimated electron-proton beam interacts with a dense, compact cloud as the jet propagates through the broad and perhaps narrow line regions (BLR and NLR) of active galactic nuclei (AGN). During the propagation of the beam through the cloud, the process of excitation of plasma waves becomes an important energy loss mechanism, especially for mildly relativistic proton beams. We compute the expected spectra of gamma-rays from the decay of neutral pions produced in hadronic collisions of the beam with the cloud, taking into account collisionless losses of the electron-proton beam. This model may explain the X-ray and TeV gamma-ray (both low and high emission states) of Mrk 421 as a result of synchrotron emission of secondary pairs from the decay of charged pions and gamma-ray emission from the decay of neutral pions for the plausible cloud parameters. However clouds can not be too hot and too dense. Otherwise the TeV gamma-rays can be attenuated by the bremsstrahlung radiation in the cloud and the secondary pairs are not able to efficiently produce synchrotron flares because of the dominant role of inverse Compton scattering. The non-variable $\gamma$-ray emission observed from Mrk 421 in the EGRET energy range cannot be described by the $\gamma$-rays from decay of neutral pions provided that the spectrum of protons in the beam is well described by a simple power law. These $\gamma$-rays might only be produced by secondary pairs scattering the soft non-variable X-rays which might originate in the inner part of the accretion disk.Comment: 14 pages,3 figures, latex, submitted to Ap