Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4·7H2O, Soya bean cake, and K2HPO4 were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH4NO3—6.63 mg/gds, MnSO4·7H2O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K2HPO4—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds

Herbal Recipes used by traditional healers towards Reproductive and Urinary healthcare in Wayanad (Kerala) India.

The traditional healers in Wayanad (Kerala), India possess rich aboriginal herbal medicinal knowledge (AHMK). This investigation has brought in to light many valuable therapeutic measures which were at the verge of extinction. Validation and documentation of some of such valuable in formation was hence done in order to conserve at least a part of this aboriginal ethno medicinal heritage. The study consists of three consecutive phases. In the first phase a methodology for the study including work plan was elucidated. A field level testing of the method was executed at a selected study site. In the second phase an extensive data collection cum field appraisal long three calendar years commences from July 2010 to July 2013 was conducted. In the final and third phase, validation and recording of the valid responses was done. A total of 60 species distributed in 56 genera belong to 38 families were identified being used in 67 recipes meant for 15 reproductive and urinary healthcare measures in Wayanad (Kerala), India. 31 herbs, 28 trees, 22 climbers and 16 shrubs were among the medicinal constituents.  The therapeutic ingredients includes  Fresh whole plants (12), dried whole plant (08), fresh root (2), dry root (29), fresh tuber (01), dried tuber (17), fresh bark (01), dried bark (02), fresh leaves (04) dried leaf (01), dried stem (01), dried petiole (01), pith powder (01),  dried gum (2), fresh inflorescence (01),  dried inflorescence (01), fresh flower (03),  dried stamen (01), dried fruits (07),  dried seeds (11), and seed oil (01).  Validity stands maximum when FPVS was four and minimum when FPVS was two. Among the 67 medicinal recipes 59 has highest FPVS and the remaining 08 has mediocre FPVS. Many of the aboriginal herbal medicinal cultures (AHMC) and the associated therapeutic knowledge and practices still alive in the district are at the verge of extinction. The present study hence pivots around the conservation issues of this aboriginal medicinal heritage, particularly in the cure and management of urinary and reproductive ailments. This improves and sustains the aboriginal therapeutic system to contribute better to the national health repository.

(R1480) Heat Transfer in Peristaltic Motion of Rabinowitsch Fluid in a Channel with Permeable Wall

This paper is intended to investigate the effect of heat transfer on the peristaltic flow of Rabinowitsch fluid in a channel lined with a porous material. The Navier -Stokes equation governs the channel\u27s flow, and Darcy\u27s law describes the permeable boundary. The Rabinowitsch fluid model\u27s governing equations are solved by utilizing approximations of the long-wavelength and small number of Reynolds. The expressions for axial velocity, temperature distribution, pressure gradient, friction force, stream function are obtained. The influence on velocity, pressure gradient, friction force, and temperature on pumping action of different physical parameters is explored via graphs

Experimental and Computational Simulation of producing ultra-fine grain Structure processed by CGP

In this research the constrained groove pressing (CGP) process as a severe plastic deformation (SPD) method was applied on commercially pure aluminum plates. According to the principle of CGP, a material is subjected to repetitive shear deformation by utilizing asymmetrically grooved dies and flat dies which are constrained by a channel. Each complete groove pre ssing pass consists of four pressing operation steps. Considering the geometry of the die, in each complete pass, a large amount of strain is induced into the specimen. In the present research the effects of the deformation passes on the mechanical propert ies of the specimens were tested by micro hardness tests. In addition, in order to investigate the material flow along the grooves in the CGP process, the finite element simulations were carried for one of the process. Our pproach involves computational simulation of the entire synthesis process for the optimization. Results show that the flow stress of the material and its hardness are affected by the number of passes. Post process of the finite element analysis showed that the real state of the CGP process is a combination of plane stress and plane strain conditions

OPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus

Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase pro¬duction using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nut¬rients for their influence on inulinase production was achieved using a Plackett––Burman design. Corn steep liquor, (NH4)2SO4, ZnSO47H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using response surface methodology (RSM). The optimum conditions are: corn steep liquor – 0.0560 (g/gds), (NH4)2SO4 – 0.0084 (g/gds), ZnSO47H2O – 0.0254 (g/gds), K2HPO4 – 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds

Effect of exogenous supplementation of GnRH or hCG on fertility in Kenguri ewes

The estimation of steroid hormone profile at different days of estrous cycle is indicative of the ovarian status of the animal. The administration of hCG on day 0 of estrous cycle and day 12 of post mating improved lambing percentage and also fertility. Among the 2 exogenous hormonal supplementations hCG therapy on day 0 and day 12 of post mating appeared to be the best in improving fertility in Kenguri ewes. The hormonal therapies are cost effective and are of great economic benefit to the farmers involving in sheep rearing

Joint action of selective herbicides used in paddy field on control of Lemna minor L.

To minimise herbicide inputs, reduce environmental burdens, and delay the evolution of herbicide resistance, it is of the utmost significance to study the interaction between herbicides. This may be accomplished by selecting the optimal combination, which, through synergy, delivers more cost-effective weed control than a single herbicide. Under laboratory conditions, interactions between binary mixtures of bensulfuron, propanil, 2,4-D, or bentazon on chlorophyll reduction of Lemna minor were investigated using the Additive Dose Model. Mixtures of propanil/2,4-D resulted in low antagonism whereas strong antagonism was evident in bensulfuron-methyl/propanil. Combinations of bensulfuron/bentazon and bensulfuron/2,4-D led to moderate antagonism. By contrast, propanil in combination with bentazon demonstrated a modest degree of synergism. The findings of this study could provide good guideline and insights when mixing two herbicides for weed control in rice fields and other aquatic environment

Performance and durability of high emittance heat receiver surfaces for solar dynamic power systems

Haynes 188, a cobalt-based superalloy, will be used to make thermal energy storage (TES) containment canisters for a 2 kW solar dynamic ground test demonstrator (SD GTD). Haynes 188 containment canisters with a high thermal emittance (epsilon) are desired for radiating heat away from local hot spots, improving the heating distribution, which will in turn improve canister service life. In addition to needing a high emittance, the surface needs to be durable in an elevated temperature, high vacuum environment for an extended time period. Thirty-five Haynes 188 samples were exposed to 14 different types of surface modification techniques for emittance and vacuum heat treatment (VHT) durability enhancement evaluation. Optical properties were obtained for the modified surfaces. Emittance enhanced samples were exposed to VHT for up to 2692 hours at 827 C and less than or equal to 10(exp -6) torr with integral thermal cycling. Optical properties were taken intermittently during exposure, and after final VHT exposure. The various surface modification treatments increased the emittance of pristine Haynes 188 from 0.11 up to 0.86. Seven different surface modification techniques were found to provide surfaces which met the SD GTD receiver VHT durability requirement. Of the 7 surface treatments, 2 were found to display excellent VHT durability: an alumina based (AB) coating and a zirconia based coating. The alumina based coating was chosen for the epsilon enhancement surface modification technique for the SD GTD receiver. Details of the performance and vacuum heat treatment durability of this coating and other Haynes 188 emittance surface modification techniques are discussed. Technology from this program will lead to successful demonstration of solar dynamic power for space applications, and has potential for application in other systems requiring high emittance surfaces

Theory and modeling of molecular modes in the NMR relaxation of fluids

Traditional theories of the NMR autocorrelation function for intramolecular dipole pairs assume single-exponential decay, yet the calculated autocorrelation of realistic systems display a rich, multi-exponential behavior resulting in anomalous NMR relaxation dispersion (i.e., frequency dependence). We develop an approach to model and interpret the multi-exponential autocorrelation using simple, physical models within a rigorous statistical mechanical development that encompasses both rotational and translational diffusion in the same framework. We recast the problem of evaluating the autocorrelation in terms of averaging over a diffusion propagator whose evolution is described by a Fokker-Planck equation. The time-independent part admits an eigenfunction expansion, allowing us to write the propagator as a sum over modes. Each mode has a spatial part that depends on the specified eigenfunction, and a temporal part that depends on the corresponding eigenvalue (i.e., correlation time) with a simple, exponential decay. The spatial part is a probability distribution of the dipole-pair, analogous to the stationary states of a quantum harmonic oscillator. Drawing inspiration from the idea of inherent structures in liquids, we interpret each of the spatial contributions as a specific molecular mode. These modes can be used to model and predict NMR dipole-dipole relaxation dispersion of fluids by incorporating phenomena on the molecular level. We validate our statistical mechanical description of the distribution in molecular modes with molecular dynamics simulations interpreted without any relaxation models or adjustable parameters: the most important poles in the Pad{\'e}-Laplace transform of the simulated autocorrelation agree with the eigenvalues predicted by the theory

Journal of Microelectronic Research - May 2003

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