Ferric citrate is a safe and digestible source of iron in broilers and piglets

Background Iron (Fe) is traditionally supplemented in poultry and swine diets using inorganic forms (e.g. sulfates, oxides). However, research suggests that organic sources are more beneficial due to greater bioavailability. In this paper, we present results from four studies aimed at assessing ferric citrate (CI-FER™, Akeso Biomedical Inc., Burlington, MA, USA) as a safe and effective source of Fe for broilers and piglets. Methods A total of four studies were performed in Germany following standard farming practices for each species. One study in day-old broiler chicks and one study in weaned piglets were designed as target animal safety studies where animals were randomly allocated to one of three treatment groups: a negative control group, the proposed dose group and a multifold dose group. Broilers and pigs were fed the experimental diets for 35 and 42 days, respectively. In each study, average daily feed intake, average daily weight gain and feed conversion ratio were measured, and blood samples were taken at study end for routine biochemistry and haematology. The other two studies were designed to evaluate different sources of dietary Fe for weaned piglets bred and managed under standard farm conditions. All piglets received routine Fe injections (200 mg Fe dextran, intramuscular) on day 3 of age, as well as the experimental diets for 42 days. In both studies, performance parameters were measured. In one study, Fe digestibility and serum Fe, superoxide dismutase and haptoglobin were also measured. In all studies, the general health status of the animals was monitored daily and all culls and mortality recorded. Each study followed a complete randomised block design. Results In broilers, ferric citrate was well tolerated up to 2,000 mg/kg feed (×10 the recommended inclusion rate) and no adverse effects on growth, blood parameters or mortality were observed. In piglets, ferric citrate was well tolerated up to 5,000 mg/kg feed (×10 the recommended inclusion rate) with no adverse effects on growth, blood parameters or mortality. In addition, piglets fed ferric citrate performed significantly better than animals fed the negative control diet (containing only endogenous Fe) and those fed inorganic forms of Fe. Moreover, piglets fed ferric citrate demonstrated improved Fe digestibility and improved oxidative status. Altogether, these findings show that ferric citrate is a safe and easily digestible source of dietary Fe for broilers and piglets

ENABLE - A Systolic 2nd Level Trigger Processor for Track Finding and e/p Discrimination for ATLAS/LHC

The Enable Machine is a systolic 2nd level trigger processor for the transition radiation detector (TRD) of ATLAS/LHC. It is developed within the EAST/RD-11 collaboration at CERN. The task of the processor is to find electron tracks and to reject pion tracks according to the EAST benchmark algorithm in less than 10 ms. Track are identified by template matching in a (f,z) region of interest (RoI) selected by a 1st level trigger. In the (f,z) plane tracks of constant curvature are straight lines. The relevant lines form mask templates. Track identification is done by histogramming the coincidences of the templates and the RoI data for each possible track. The Enable Machine is an array processor that handles tracks of the same slope in parallel, and tracks of different slope in a pipeline. It is composed of two units, the Enable histogrammer unit and the Enable z/f-board. The interface daughter board is equipped with a HIPPI-interface developed at JINR/Dubna, and Xilinx 'corner turning' data converter chips. Enable uses programmable gate arrays (XILINX) for histogramming and synchronous SRAMs for pattern storage. With a clock rate of 40 MHz the trigger decision time is 6.5 ms and the latency 7.0 ms. The Enable machine is scalable in the RoI size as well as in the number of tracks processed. It can be adapted to different recognition tasks and detector setups. The prototype of the Enable Machine has been tested in a beam time of the RD6 collaboration at CERN in October 1993

Extremal Codes for Speed-up of Distributed Parallel Arbitration

This paper describes a method that allows the speed up of parallel processes in distributed arbitration schemes as used in Futurebus+. It is based on special arbitration codes that decrease the maximal arbitration time to a specified value. Such codes can be applied with few, if any, minor changes of the hardware. The general structure of these codes is given

Results of On-Line Tests of the ENABLE Prototype, a 2nd Level Trigger Processor for the TRT of ATLAS/LHC

The Enable Machine is a systolic 2nd level trigger processor for the transition radiation detector (TRD) of ATLAS/LHC. The task of the processor is to find the best candidate for a lepton track in a high background of pions according to the EAST benchmark [2] in less than 10 5s. As described earlier [1, 2] the Enable Machine finds all reasonable tracks by histogramming the coincidence of the mask templates and the RoI for each track. A prototype has been developed and tested within the EAST/RD-11 collaboration at CERN. It operates at 50 MHz and finds up to 400 arbitrary tracks in less than 10 5s. It is assembled of an interface board and one or more histogrammer boards which makes the Enable Machine easily scalable. The histogrammer units are systolic arrays consisting of a matrix of 36 field- programmable gate arrays (Xilinx XC3190). Through this it is possible to optimize the trigger algorithm, to adapt it to a changed detector setup, and it allows even the implementation of completly new algorithms. For the beam tests in autumn 1993 at CERN the overall functionality within the detector environment could be shown. We were able to link successfully the Enable prototype to the detector raw data stream as well as to the data acquisition system. For the next beam period in 1994 we will focus on efficiency measurements and tests with maximal detector data rate

Enable++ : a second generation FPGA processor

In the computing community field programmable processors are going to fill the niche for special purpose computing devices. A typical example is ultra-fast pattern recognition in experimental particle physics - a task for which we constructed two years ago Enable- 1, an FPGA processor rather specialized for pattern recognition algorithms in μs domain, but also provided with modest features for coping with more general applications. This paper presents the follow-up modell Enable++, a 2nd generation FPGA processor that offers several substantial enhancements over the previous system for a wider range of applications: Enable++ is structured into three different state-of-the-art modules for providing computing power, flexible and high-speed I/O communication and powerful intermodule communication with a raw bandwidth of 3.2 GByte/s by an active backplane. The technical realization of all three modules is guided by the maximum usage of field programmable logic. The actual demand of computing-and I/O-power can be satisified by the number of modules plugged into the crate. Enhanced features of Enable++ comprise the configurable processor topology provided by programmable crossbar switches. In combination with the 4 x 4 FPGA array and 12 MByte distributed RAM the Enable++ computing core offers a strongly increased and scalable computing power. For building new applications the system offers a comfortable programming and debugging environment consisting of a compiler for the C-like hardware description language spC, a simulator and a source level debugger for hardware design. The goal in planning the hardware design environment for Enable++ from scratch is to transfer established methodologies in software design to the design of digital logic. Concerning pattern recognition tasks, we estimate that Enable++ surpasses modern RISC processors by a factor of 100 to 1000

Fiber Composition in Sows’ Diets Modifies Clostridioides difficile Colonization in Their Offspring

Dietary fiber has a potential to modulate the gut microbiota in sows. We hypothesized that a maternal diet rich in either high- or low-fermentable fiber during gestation and lactation influences Clostridioides difficile gut colonization in suckling piglets. Twenty sows were fed gestation and lactation diets enriched with either high-fermentable sugar beet pulp (SBP) or low-fermentable lignocellulose (LNC) fibers. C. difficile, toxin B (TcdB), fecal score, microbial abundance (16S-rDNA sequencing) and metabolites were measured in the feces from the sows and their piglets. C. difficile concentration was higher in piglets from the sows fed LNC than SBP along the study (P ≤ 0.05). Higher prevalence of C. difficile was noted in three-week-old piglets from sows fed LNC vs. SBP (45% vs. 0%, P = 0.001). TcdB prevalence was higher in six-day-old piglets from the sows fed LNC vs. SBP (60% vs. 17%, P = 0.009). In sows, fecal microbial metabolites were higher in SBP than LNC, while C. difficile concentration showed no difference. Higher microbial diversity Shannon index was noted in sows from SBP vs. LNC one week before parturition and at the parturition (P ≤ 0.05). Piglets from SBP vs. LNC tended to have higher microbial diversity Shannon index at two and three weeks of age. Diets enriched with high-fermentable fiber compared to low-fermentable fiber in sows reduced C. difficile colonization in their piglets. Susceptibility to colonization by C. difficile in neonatal piglets can be modulated by the sows’ diet, supporting the hypothesis of the early microbial programming in the offspring and the importance of the sow-piglet couple

Spurenelementversorgung bei Milchrindern

In den Mischfutterrationen für Milchrinder wurde ein mittlerer Gehalt von Kupfer, Zink, Mangan und Selen ermittelt, der 2,5-fach über der Versorgungsempfehlung liegt. Neben den umweltrelevanten Konflikten dieser Praxis sind auch Versorgungsprobleme bei den Milchrindern aufgrund unerwünschter Interaktionen der Elemente nicht auszuschließen. In elf sächsischen Milchviehbetrieben wurden die Ursachen der Überversorgung untersucht und Vorschläge zur Optimierung der Spurenelementversorgung von Milchrindern erarbeitet. Dabei konnte die mittlere Kupfer-, Zink- und Selenkonzentration in den TMR um im Mittel 25 %, d. h. auf ein mittleres Versorgungsniveau der Tiere von 150 % der Versorgungsempfehlung reduziert werden. Das Heft fasst die Ergebnisse der Untersuchungen zusammen

Tolerance and safety evaluation of N, N-dimethylglycine, a naturally occurring organic compound, as a feed additive in broiler diets

N,N-dimethylglycine (DMG) is a tertiary amino acid that naturally occurs as an intermediate metabolite in choline-to-glycine metabolism. The objective of the present trial was to evaluate tolerance, safety and bioaccumulation of dietary DMG in broilers when supplemented at 1 g and 10 g Na-DMG/kg. A feeding trial was conducted using 480 1-d-old broiler chicks that were randomly allocated to twenty-four pens and fed one of three test diets added with 0, 1 or 10 g Na-DMG/kg during a 39 d growth period. Production performance was recorded to assess tolerance and efficacy of the supplement. At the end of the trial, toxicity was evaluated by means of haematology, plasma biochemistry and histopathology of liver, kidney and heart (n 12), whereas bioaccumulation was assessed on breast meat, liver, blood, kidney and adipose tissue (n 8). Carcass traits were similar between the control and 1 g Na-DMG/kg feed groups (P.0·05), but the feed:gain ratio was significantly improved at 1 g Na-DMG/kg feed compared with the control or the 10-fold dose (P¼0·008). Histological examinations showed no pathological effects and results of haematology and plasma biochemistry revealed similar values between the test groups (P.0·05). Bioaccumulation occurred at the 10-fold dose, but the resulting DMG content in breast meat was comparable with, for instance, wheat bran and much lower than uncooked spinach. In conclusion, DMG at 1 g Na-DMG/kg improved the feed:gain ratio in broilers without DMG being accumulated in consumer parts. Furthermore, dietary supplementation with DMG up to 10 g Na-DMG/kg did not induce toxicity or impaired performance in broilers

Clostridioides difficile-mesocolonic oedema in neonatal suckling piglets develops regardless of the fibre composition in sow’s diets

Maternal dietary factors have been reported to influence Clostridioides difficile colonisation in the offspring. Twenty suckling piglets from sows fed diets supplemented with high-fermentable sugar beet pulp (SBP) or low-fermentable lignocellulose (LNC) fibres during gestation and lactation were dissected in the first week after birth. Postmortem analysis included clinical mesocolon and faecal scoring, concentration of C. difficile and respective toxins in colon digesta and faeces, immunoglobulins in serum and inflammatory markers in serum and colon tissues. Sow colostrum was assessed for nutrients, immunoglobulins and biogenic amines. Toxin-neutralising IgG antibodies were measured in colostrum and serum of the sows, and in colon digesta and serum of the piglets. Mesocolonic oedema of different severity was present in most of the piglets from both sows’ feeding groups. Concentrations of C. difficile, toxins and calprotectin in colon digesta and faecal contents did not differ between the study piglets. Calprotectin correlated positively with mesocolon score (rho = 413, P = 0.07). Piglets from sows fed LNC vs SBP tended to have higher IgA (P = 0.089), IgG (P = 0.053), total Ig (P = 0.053), albumin (P = 0.075) and total protein content (P = 0.007) in serum. Colon tissues of piglets from the SFB vs LNC had upregulated expression of ZO-1 (P = 0.021), PCNA (P = 0.015) and TGF-β (P = 0.014). Titers of anti-toxin-IgG-antibodies in serum and colostrum and in piglet colon digesta and serum did not differ between sows from both dietary groups, but they all showed strong positive correlations. In conclusion, dietary sugar beet pulp or lignocellulose fed to sows did not influence the concentrations of C. difficile and toxins titers in colon digesta and faeces of neonatal piglets