KLK10 (kallikrein-related peptidase 10)

Review on KLK10 (kallikrein-related peptidase 10), with data on DNA, on the protein encoded, and where the gene is implicated

Incremental Contributions of FbaA and Other Impetigo-Associated Surface Proteins to Fitness and Virulence of a Classical Group A Streptococcal Skin Strain

Group A streptococci (GAS) are highly prevalent human pathogens whose primary ecological niche is the superficial epithelial layers of the throat and/or skin. Many GAS strains having a strong tendency to cause pharyngitis are distinct from strains that tend to cause impetigo; thus, genetic differences between them may confer host tissue-specific virulence. In this study, the FbaA surface protein gene is found to be present in most skin specialist strains, but largely absent from a genetically-related subset of pharyngitis isolates. Using an DeltafbaA mutant constructed in the impetigo strain Alab49, loss of FbaA resulted in a slight but significant decrease in GAS fitness in a humanized mouse model for impetigo; the DeltafbaA mutant also exhibited decreased survival in whole human blood due to phagocytosis. Using assays with highly sensitive outcome measures, Alab49DeltafbaA was compared to other isogenic mutants lacking virulence genes known to be disproportionately associated with classical skin strains. FbaA and PAM (i.e., M53 protein) have additive effects in promoting GAS survival in whole blood. The pilus adhesin tip protein Cpa promotes Alab49 survival in whole blood, and appears to fully account for the antiphagocytic effect attributable to pili. That numerous skin strain-associated virulence factors make slight but significant contributions to virulence underscores the incremental contributions to fitness of individual surface protein genes and the multifactorial nature of GAS-host interactions

Biofuel trigeneration with energy storage for heating, cooling and power on farms

The drive towards net-zero carbon emissions has prompted many industries to alter the way they operate. The agriculture industry is responsible for a large proportion of the UK’s greenhouse gas emissions. Thus, the feasibility of implementing an anaerobic digestion (AD) system supplying biogas to a trigeneration system with energy storage for the provision of heating, cooling and power has been investigated in the context of a medium-scale arable farm. Two configurations – one supplied with wheat straw only, and the other supplied with wheat straw, barley straw and manure – were identified to meet the energy demands of the farm. Technical feasibility was investigated via simulations run in ECLIPSE, with the two configurations returning overall system efficiencies of 66.8% and 67.1%, respectively. Economic analyses indicated simple payback periods of 9.4 and 11 years, which fall within the expected 20-year lifetime of the project. Furthermore, the potential reduction in CO2 emissions for each scenario was determined to be 42,764 kg and 43,956 kg per year

Higher expression of human kallikrein 10 in breast cancer tissue predicts tamoxifen resistance

The human tissue kallikreins are secreted serine proteases, encoded by a group of homologous genes clustered in tandem on chromosome 19q13.3-4. Human kallikrein 6 and human kallikrein 10 are two new members of this family. Recently, we developed highly sensitive and specific immunofluorometric assays for human kallikrein 6 and human kallikrein 10, which allow for their quantification in tissue extracts and biological fluids. Both human kallikrein 6 and human kallikrein 10 are found to be down-regulated in breast cancer cell lines, suggesting that they may be involved in breast cancer pathogenesis and progression. In this study, we investigated the potential value of human kallikrein 6 and human kallikrein 10 as prognostic and predictive factors in breast cancer. We quantified human kallikrein 6 and human kallikrein 10 protein levels in 749 breast tumour cytosolic extracts and correlated this data with various clinicopathological variables and patient outcomes. Human kallikrein 6 and human kallikrein 10 are positively correlated with each other. Higher human kallikrein 6 and human kallikrein 10 protein levels are associated with younger age, pre-menopausal, status and tumours which are negative for oestrogen and progesterone receptors. No correlation was found between human kallikrein 6 and human kallikrein 10 levels and tumour size, grade, and nodal status. Survival analysis showed that neither human kallikrein 6 nor human kallikrein 10 are related to the rate of relapse-free and overall survival. In the analysis with respect to response to tamoxifen therapy, although human kallikrein 6 levels were not associated with tamoxifen responsiveness, higher levels of human kallikrein 10 were significantly associated with a poor response rate. This association remained significant in the multivariate analysis. Furthermore, higher human kallikrein 10 levels were significantly related with a short progression-free and post-relapse overall survival after start of tamoxifen treatment for advanced disease. Taken together, our results suggest that although human kallikrein 6 and human kallikrein 10 are not prognostic markers for breast cancer, human kallikrein 10 is an independent predictive marker for response of tamoxifen therapy

Performance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS systems was not officially launched for diagnostic use in clinical microbiology laboratories in China until 2012. Here, we report the findings from the first large-scale evaluation study of VITEK MS for routine bacterial identification in two major diagnostic centres in Beijing and Hong Kong. A total of 2266 unique isolates representing 56 genera and 127 species were analysed, and results were compared to those obtained by VITEK 2. Any discrepancies were resolved by 16S rRNA sequencing. Overall, VITEK MS provided correct identification for 2246 (99.1%) isolates, including 2193 (96.8%) with correct species-level identifications and 53 (2.3%) matched at the genus level only. VITEK MS surpassed VITEK 2 consistently in species-level identification of important pathogens, including non-Enterobacteriaceae Gram-negative bacilli (94.7 versus 92%), staphylococci (99.7 versus 92.4%), streptococci (92.6 versus 79.4%), enterococci (98.8 versus 92.6%) and Clostridium spp. (97.3 versus 55.5%). The findings demonstrated that VITEK MS is highly accurate and reliable for routine bacterial identification in clinical settings in China. © 2015 The Authors.postprin

Use of experimental sensors for discrimination of artificial breath mixtures in a lung cancer screening context

peer reviewedThe Pathacov project [3] aims at creating an electronic nose to detect lung cancer in the population at risk. Metal oxide sensors are being developed within the project to better detect cancer biomarkers, which are identified within a large-scale clinical study in university hospitals in the north-east of France. Before long and costly clinical trials, the performance of the prototype electronic nose has to be evaluated. In order to do this, a novel approach has been implemented. Real breath samples from healthy and available volunteers are collected in Teflon FEP sampling bags. The results of the electronic nose are processed through a principal component analysis (PCA) in order to evaluate the contribution of each sensor to the separation of the group’s clusters (healthy and cancer). This enables the selection of the best performing sensors to be included in the final prototype.Pathaco

Expression Analysis and Interaction Protein Screening of CRY1 in Strawberry

Cryptochrome 1 (CRY1), a main blue light receptor protein, plays a significant role in several biological processes. However, the expression patterns and function of CRY1 in strawberry have not been identified. Here, the expression profile of CRY1 in different tissues and developmental stages of strawberry fruit, and expression patterns response to abiotic stresses (low temperature, salt and drought) were analyzed. Its subcellular localization, interaction proteins and heterologous overexpression in tobacco were also investigated. The results showed that CRY1 was mainly expressed in leaves and fruits with an expression peak at the initial red stage in strawberry fruit. Abiotic stresses could significantly induce the expression of CRY1. The CRY1 protein was located in both nucleus and cytoplasm. Five proteins (CSN5a-like, JAZ5, eIF3G. NF-YC9, and NDUFB9) interacting with CRY1 were discovered. Genes related flowering times, such as HY5 and CO, in three overexpressed FaCRY1 tobacco lines, were significantly upregulated. Taken together, our results suggested CRY1 have a broad role in biological processes in strawberry