4 research outputs found
Engineered Protein-Driven Synthesis of Tunable Iron Oxide Nanoparticles as T1 and T2 Magnetic Resonance Imaging Contrast Agents
Iron oxide nanoparticles (IONPs) have become one of the
most promising
nanomaterials for biomedical applications because of their biocompatibility
and physicochemical properties. This study demonstrates the use of
protein engineering as a novel approach to design scaffolds for the
tunable synthesis of ultrasmall IONPs. Rationally designed proteins,
containing different number of metal-coordination sites, were evaluated
to control the size and the physicochemical and magnetic properties
of a set of protein-stabilized IONPs (Prot-IONPs). Prot-IONPs, synthesized
through an optimized coprecipitation approach, presented good T1 and
T2 relaxivity values, stability, and biocompatibility, showing potential
for magnetic resonance imaging (MRI) applications
Insight into Serum Protein Interactions with Functionalized Magnetic Nanoparticles in Biological Media
Surface modification with linear polymethacrylic acid (20 kDa),
linear and branched polyethylenimine (25 kDa), and branched oligoethylenimine
(800 Da) is commonly used to improve the function of magnetite nanoparticles
(MNPs) in many biomedical applications. These polymers were shown
herein to have different adsorption capacity and anticipated conformations
on the surface of MNPs due to differences in their functional groups,
architectures, and molecular weight. This in turn affects the interaction
of MNPs surfaces with biological serum proteins (fetal bovine serum).
MNPs coated with 25 kDa branched polyethylenimine were found to attract
the highest amount of serum protein while MNPs coated with 20 kDa
linear polymethacrylic acid adsorbed the least. The type and amount
of protein adsorbed, and the surface conformation of the polymer was
shown to affect the size stability of the MNPs in a model biological
media (RPMI-1640). A moderate reduction in <i>r</i><sub>2</sub> relaxivity was also observed for MNPs suspended in RPMI-1640
containing serum protein compared to the same particles suspended
in water. However, the relaxivities following protein adsorption are
still relatively high making the use of these polymer-coated MNPs
as Magnetic Resonance Imaging (MRI) contrast agents feasible. This
work shows that through judicious selection of functionalization polymers
and elucidation of the factors governing the stabilization mechanism,
the design of nanoparticles for applications in biologically relevant
conditions can be improved
One-Step Fast Synthesis of Nanoparticles for MRI: Coating Chemistry as the Key Variable Determining Positive or Negative Contrast
Iron oxide nanomaterial
is a typical example of a magnetic resonance
imaging probe for negative contrast. It has also been shown how this
nanomaterial can be synthesized for positive contrast by modification
of the composition and size of the core. However, the role of the
organic coating in the relaxometric properties is largely unexplored.
Here, maghemite nanoparticles with either excellent positive or very
good negative contrast performance are obtained by modifying coating
thickness while the core is kept unchanged. Different nanoparticles
with tailored features as contrast agent according to the coating
layer thickness have been obtained in a single-step microwave-driven
synthesis by heating at different temperatures. A comprehensive analysis
is conducted of how the composition and structure of the coating affects
the final magnetic, relaxometric, and imaging performance. These results
show how the organic coating plays a fundamental role in the intrinsic
relaxometric parameters of iron oxide-based contrast media
Effect of Surface Chemistry and Associated Protein Corona on the Long-Term Biodegradation of Iron Oxide Nanoparticles In Vivo
The protein corona
formed on the surface of a nanoparticle in a biological medium determines
its behavior in vivo. Herein, iron oxide nanoparticles containing
the same core and shell, but bearing two different surface coatings,
either glucose or poly(ethylene glycol), were evaluated. The nanoparticles’
protein adsorption, in vitro degradation, and in vivo biodistribution
and biotransformation over four months were investigated. Although
both types of nanoparticles bound similar amounts of proteins in vitro,
the differences in the protein corona composition correlated to the
nanoparticles biodistribution in vivo. Interestingly, in vitro degradation
studies demonstrated faster degradation for nanoparticles functionalized
with glucose, whereas the in vivo results were opposite with accelerated
biodegradation and clearance of the nanoparticles functionalized with
poly(ethylene glycol). Therefore, the variation in the degradation
rate observed in vivo could be related not only to the molecules attached
to the surface, but also with the associated protein corona, as the
key role of the adsorbed proteins on the magnetic core degradation
has been demonstrated in vitro