C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-5

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>with neurons expressing Dvl and Gsk3β. Scale bar 15 μm. Quantification shows that neurons expressing Dvl and Gsk3β and treated with JNK inhibitor have a decreased level of MTs stability than untreated neurons expressing Dvl and Gsk3β. Quantification shows that neurons expressing Dvl together with Gsk3β and treated with Anisomicin have comparable level of MT stability as untreated neurons. Inhibition of JNK by expression of JBD does not affect the level of phosphorylated MAP1B in granule cell neurons expressing Dvl when compared to control neurons expressing Dvl alone (arrow). Scale bar 10 μm. Two asterisks, < 0.01; Three asterisks, < 0.001

C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-4

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>ated with or without the JNK inhibitor SP600125 and MT stability was examined after Nocodazole treatment. Quantification shows that neurons expressing ΔDIX or ΔPDZ mutants and treated with SP600125 exhibit the same low level of MT stability as untreated neurons. Conversely, neurons expressing ΔDEP-Dvl mutant and treated with SP600125 show lower level of MT stability than control untreated neurons. C) Full length Dvl and ΔDEP-Dvl bind to MTs along the axon whereas low levels of ΔPDZ-Dvl mutant remains associated to MTs after detergent fixation. Scale bar 15 μm. Three asterisks, < 0.001

C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-3

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>of endogenous JNK activity, when compared to controls. Low levels of Anisomicin also increase the level of p-c-Jun when compared with control untreated EGFP-expressing neurons or neurons exposed to β-estradiol for three hrs. However, the levels of total c-Jun remain unchanged. Quantification of three independent experiments shows that induction of Dvl-ER expression induces a 40% increase in p-c-Jun levels compare to control. Expression of Dvl increases the level of p-JNK decorating the MT network along neurites when compared to EGFP-expressing control neurons. Pseudocolor panels show Dvl increases the level of p-JNK associated with microtubules. Scale bar 5 μm. Two asterisks, < 0.01

C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-6

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>phorylation of MAP1B and increased microtubule stability. Neurons expressing Dvl contain stable MTs after Nocodazole treatment. Expression of Gsk3β partially blocks the ability of Dvl to stabilize MTs. Quantification shows that Dvl alone induces MT stability when compared to control EGFP-expressing neurons. Neurons expressing Gsk3β alone does not induce Mt stability while neurons expressing both Gsk3β and Dvl exhibit decreased MT stability when compared to Dvl-expressing neurons. Scale bar: 15 μm. Three asterisks, < 0.001; For asterisks, < 0.0001

C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-1

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>g neurons. Expression of dominant negative Rho (RhoN19), dominant negative Rac (RacN17), and dominant negative Ccd42 (Cdc42 N17) do not alter the ability of Dvl to stabilise microtubules. In addition, neurons expressing Dvl and treated with the ROCK inhibitor Y27632 exhibit comparable level of microtubule stability as untreated Dvl-expressing neurons. All neurons were treated with Nocodazole to test MT stability Quantification shows that coexpression of Dvl together with dominant negative Rac Rho, or Cdc42 or the ROCK inhibitor does not change the ability of Dvl to stabilise MTs. Scale bar: 15 μm

C-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability-2

<p><b>Copyright information:</b></p><p>Taken from "c-Jun N-terminal kinase (JNK) cooperates with Gsk3β to regulate Dishevelled-mediated microtubule stability"</p><p>http://www.biomedcentral.com/1471-2121/8/27</p><p>BMC Cell Biology 2007;8():27-27.</p><p>Published online 3 Jul 2007</p><p>PMCID:PMC1940000.</p><p></p>e stability when compared to EGFP-expressing neurons. Neurons expressing JNK alone reveals a level of stability comparable to that one obtained after exposure of neurons to Anisomicin. Quantification shows that Anisomicin treatment and JNK expression increase the level of MT stability in neurons Dvl-expressing neurons treated with the JNK inhibitor SP600125 or expressing the dominant negative JNK (JBD) show decreased MT stability when compared to control Dvl-expressing neurons. Quantification shows that treatment of Dvl expressing neurons with JNK inhibitor SP 600125 or expression of JBD reduces the level of MT stability. Scale bar 15 μm. Three asterisks, < 0.001; For asterisks, < 0.0001