Root anatomical traits contribute to deeper rooting of maize under compacted field conditions

© The Author(s) 2020. To better understand the role of root anatomy in regulating plant adaptation to soil mechanical impedance, 12 maize lines were evaluated in two soils with and without compaction treatments under field conditions. Penetrometer resistance was 1–2 MPa greater in the surface 30 cm of the compacted plots at a water content of 17–20% (v/v). Root thickening in response to compaction varied among genotypes and was negatively associated with rooting depth at one field site under non-compacted plots. Thickening was not associated with rooting depth on compacted plots. Genotypic variation in root anatomy was related to rooting depth. Deeper-rooting plants were associated with reduced cortical cell file number in combination with greater mid cortical cell area for node 3 roots. For node 4, roots with increased aerenchyma were deeper roots. A greater influence of anatomy on rooting depth was observed for the thinner root classes. We found no evidence that root thickening is related to deeper rooting in compacted soil; however, anatomical traits are important, especially for thinner root classes

Effects of soil structure complexity to root growth of plants with contrasting root architecture

This study was funded by the Scottish Food Security Alliance a partnership between the University of Aberdeen, University of Dundee and The James Hutton Institute. We thank all people that contributed to this work. In particular, Rong Qu, Dhin Md Islam, Dr. Stewart J Chalmers, Annette Raffan and Jaime Buckingham for providing technical support.Peer reviewedPublisher PD

The helical motions of roots are linked to avoidance of particle forces in soil

Limitation to root growth results from forces required to overcome soil resistance to deformation. The variations in individual particle forces affects root development and often deflects the growth trajectory. We have developed transparent soil and optical projection tomography microscopy systems where measurements of growth trajectory and particle forces can be acquired in a granular medium at a range of confining pressures. We developed image-processing pipelines to analyse patterns in root trajectories and a stochastic-mechanical theory to establish how root deflections relate to particle forces and thickening of the root. Root thickening compensates for the increase in mean particle forces but does not prevent deflections from 5% of most extreme individual particle forces causing root deflection. The magnitude of deflections increases with pressure but they assemble into helices of conserved wavelength in response linked to gravitropism. The study reveals mechanisms for the understanding of root growth in mechanically impeding soil conditions and provides insights relevant to breeding of drought-resistant crops.</p

Soil penetration by maize roots is negatively related to ethylene-induced thickening

Radial expansion is a classic response of roots to a mechanical impedance that has generally been assumed to aid penetration. We analysed the response of maize nodal roots to impedance to test the hypothesis that radial expansion is not related to the ability of roots to cross a compacted soil layer. Genotypes varied in their ability to cross the compacted layer, and those with a steeper approach to the compacted layer or less radial expansion in the compacted layer were more likely to cross the layer and achieve greater depth. Root radial expansion was due to cortical cell size expansion, while cortical cell file number remained constant. Genotypes and nodal root classes that exhibited radial expansion in the compacted soil layer generally also thickened in response to exogenous ethylene in hydroponic culture, that is, radial expansion in response to ethylene was correlated with the thickening response to impedance in soil. We propose that ethylene insensitive roots, that is, those that do not thicken and can overcome impedance, have a competitive advantage under mechanically impeded conditions as they can maintain their elongation rates. We suggest that prolonged exposure to ethylene could function as a stop signal for axial root growth

The ability of maize roots to grow through compacted soil is not dependent on the amount of roots formed

© 2020 Elsevier B.V. Mechanical impedance is a primary constraint to root growth and hence the capture of soil resources. To investigate whether rooting depth and root length under mechanical impedance caused by compaction are correlated we evaluated 12 maize lines at two field sites. To distinguish between lateral and nodal roots, roots were sorted into different diameter classes. Coarse roots had diameters >1 mm and represent nodal root axes. Greater proportions of coarse roots on compacted plots were found at both field sites however results were driven by genotypic variation. Soil compaction reduced total rooting depth (in all diameter classes) and coarse rooting depth at both sites compared to non-compacted plots. Root distribution was influenced by compaction with greater root length densities closer to the soil surface. Root length and root depth were not related to each other under impeded conditions. Coarse roots of some genotypes became obstructed on the compacted plots, while other genotypes were capable of growing through the impeding soil and reached deeper soil strata resulting in differential distribution of roots through the soil profile. On compacted plots we observed genotypes with similar root depths but with contrasting coarse root lengths. The ability of roots to grow through compacted soils is therefore not dependent solely on the coarse root length formed by the root system

Ecological mitigation of hillslope instability: ten key issues facing researchers and practitioners

Background Plants alter their environment in a number of ways. With correct management, plant communities can positively impact soil degradation processes such as surface erosion and shallow landslides. However, there are major gaps in our understanding of physical and ecological processes on hillslopes, and the application of research to restoration and engineering projects. Scope To identify the key issues of concern to researchers and practitioners involved in designing and implementing projects to mitigate hillslope instability, we organized a discussion during the Third International Conference on Soil Bio- and Eco-Engineering: The Use of Vegetation to Improve Slope Stability, Vancouver, Canada, July 2012. The facilitators asked delegates to answer three questions: (i) what do practitioners need from science? (ii) what are some of the key knowledge gaps? (iii) what ideas do you have for future collaborative research projects between practitioners and researchers? From this discussion, ten key issues were identified, considered as the kernel of future studies concerning the impact of vegetation on slope stability and erosion processes. Each issue is described and a discussion at the end of this paper addresses how we can augment the use of ecological engineering techniques for mitigating slope instability. Conclusions We show that through fundamental and applied research in related fields (e.g., soil formation and biogeochemistry, hydrology and microbial ecology), reliable data can be obtained for use by practitioners seeking adapted solutions for a given site. Through fieldwork, accessible databases, modelling and collaborative projects, awareness and acceptance of the use of plant material in slope restoration projects should increase significantly, particularly in the civil and geotechnical communities. (Résumé d'auteur